Downregulation of Msx-2 expression results in chondrogenesis in the medial region of the avian mandible.
Identifieur interne : 003E82 ( Main/Merge ); précédent : 003E81; suivant : 003E83Downregulation of Msx-2 expression results in chondrogenesis in the medial region of the avian mandible.
Auteurs : M. Mina [États-Unis] ; J. Gluhak ; B. RodgersSource :
- Connective tissue research [ 0300-8207 ] ; 1996.
Descripteurs français
- KwdFr :
- ARN messager (analyse), Animaux, Cartilage (), Cartilage (embryologie), Cartilage (métabolisme), Embryon de poulet, Expression des gènes, Mandibule (), Mandibule (embryologie), Mandibule (métabolisme), Oligonucléotides antisens (pharmacologie), Protéines de liaison à l'ADN (génétique), Protéines à homéodomaine, Régulation négative, Techniques de culture d'organes.
- MESH :
- analyse : ARN messager.
- embryologie : Cartilage, Mandibule.
- génétique : Protéines de liaison à l'ADN.
- métabolisme : Cartilage, Mandibule.
- pharmacologie : Oligonucléotides antisens.
- Animaux, Cartilage, Embryon de poulet, Expression des gènes, Mandibule, Protéines à homéodomaine, Régulation négative, Techniques de culture d'organes.
English descriptors
- KwdEn :
- Animals, Cartilage (drug effects), Cartilage (embryology), Cartilage (metabolism), Chick Embryo, DNA-Binding Proteins (genetics), Down-Regulation, Gene Expression, Homeodomain Proteins, Mandible (drug effects), Mandible (embryology), Mandible (metabolism), Oligonucleotides, Antisense (pharmacology), Organ Culture Techniques, RNA, Messenger (analysis).
- MESH :
- chemical , analysis : RNA, Messenger.
- chemical , genetics : DNA-Binding Proteins.
- drug effects : Cartilage, Mandible.
- embryology : Cartilage, Mandible.
- metabolism : Cartilage, Mandible.
- chemical , pharmacology : Oligonucleotides, Antisense.
- Animals, Chick Embryo, Down-Regulation, Gene Expression, Homeodomain Proteins, Organ Culture Techniques.
Abstract
Homeobox-containing genes are thought to be regulators of pattern formation during vertebrate development. We have previously characterized regions in the developing chick mandible expressing either Msx-1 or Msx-2 in terms of their chondrogenic potential, rates of cell proliferation, and their correlation with regions of cell death. These experiments suggest that Msx-1 may be involved in outgrowth of the mandibular arch, and Msx-2 may be involved in delineating the non-chondrogenic region of the medial part of the mandibular arch. To further examine the possibility that expression of Msx-2 may be involved in preventing chondrogenesis in the medial region, mandibular arch explants from stage 23 chick embryos were cultured for 4 days in media in the absence of serum but in the presence of 20-30 microM Msx-2 sense or antisense oligonucleotides (18 mers). In explants grown in either control media or with the sense oligonucleotide two rods of cartilage separated by a cartilage free area located in the medial region of the mandible were formed. In explants treated with Msx-2 antisense oligonucleotide cartilage formation was observed in the medial region of the mandible resulting in the fusion of the two bilateral rods at the midline. These results suggest a negative relationship between Msx-2 expression and chondrogenesis in the medial region of the developing mandible.
DOI: 10.3109/03008209609029177
PubMed: 9084645
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pubmed:9084645Le document en format XML
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<front><div type="abstract" xml:lang="en">Homeobox-containing genes are thought to be regulators of pattern formation during vertebrate development. We have previously characterized regions in the developing chick mandible expressing either Msx-1 or Msx-2 in terms of their chondrogenic potential, rates of cell proliferation, and their correlation with regions of cell death. These experiments suggest that Msx-1 may be involved in outgrowth of the mandibular arch, and Msx-2 may be involved in delineating the non-chondrogenic region of the medial part of the mandibular arch. To further examine the possibility that expression of Msx-2 may be involved in preventing chondrogenesis in the medial region, mandibular arch explants from stage 23 chick embryos were cultured for 4 days in media in the absence of serum but in the presence of 20-30 microM Msx-2 sense or antisense oligonucleotides (18 mers). In explants grown in either control media or with the sense oligonucleotide two rods of cartilage separated by a cartilage free area located in the medial region of the mandible were formed. In explants treated with Msx-2 antisense oligonucleotide cartilage formation was observed in the medial region of the mandible resulting in the fusion of the two bilateral rods at the midline. These results suggest a negative relationship between Msx-2 expression and chondrogenesis in the medial region of the developing mandible.</div>
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