Electrospray tandem mass spectrometry of nucleotides.
Identifieur interne : 003E26 ( Main/Exploration ); précédent : 003E25; suivant : 003E27Electrospray tandem mass spectrometry of nucleotides.
Auteurs : J. Boschenok [Australie] ; M M SheilSource :
- Rapid communications in mass spectrometry : RCM [ 0951-4198 ] ; 1996.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Oligonucleotides.
- Base Sequence, Electrochemistry, Mass Spectrometry, Molecular Sequence Data.
Abstract
The tandem mass spectra (MS/MS) following electrospray ionization of nucleotides (from 2- to 6-mers) have been examined under a range of collision and ionization conditions. In general, abundant peaks due to the free bases and phosphate groups are observed, along with a limited number of sequence ions. A number of strategies for increasing the extent of the observed fragmentation were investigated. Increasing the skimmer potential of the ion source, thereby increasing the initial internal energy of the incident ions, did not significantly enhance the sequence-specific fragmentation, although the relative intensities of fragment ions increased. In MS/MS spectra of different adducts (i.e. [M + H]+, [M-H]-) of dinucleotides the intensity of the ions due to the bases (BH2+ or B-) is dependent on both the base position (i.e. either 3' or 5') and the nature of the adduct ion. These data show that MS/MS spectra of [M-H]- ions of dinucleotides generated by electrospray ionization may be equally as diagnostic as MS/MS spectra obtained following fast-atom bombardment. With [M + Li]+ ions, more sequence ions were observed compared to the corresponding protonated and deprotonated species. For oligonucleotide hexamers, MS/MS spectra of the [M-2H]2- ions show fragment ions due to loss of bases followed by cleavage of the 3' C--O bond in the sugar from which the base is lost (i.e. w ions); however, a strong preference for loss of any particular base is not observed. Finally, MS/MS spectra of 5'-ApCpC-3' and 5'-d(AGGCCT)-3' obtained on a magnetic sector orthogonal acceleration time-of-flight mass spectrometer demonstrate the potential utility of this instrumental configuration for MS/MS analysis of oligonucleotides.
DOI: 10.1002/(SICI)1097-0231(19960115)10:1<144::AID-RCM452>3.0.CO;2-D
PubMed: 8563014
Affiliations:
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Boschenok</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, University of Wollongong, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Chemistry, University of Wollongong, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author><author><name sortKey="Sheil, M M" sort="Sheil, M M" uniqKey="Sheil M" first="M M" last="Sheil">M M Sheil</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:8563014</idno><idno type="pmid">8563014</idno><idno type="doi">10.1002/(SICI)1097-0231(19960115)10:1<144::AID-RCM452>3.0.CO;2-D</idno><idno type="wicri:Area/PubMed/Corpus">002790</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002790</idno><idno type="wicri:Area/PubMed/Curation">002790</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002790</idno><idno type="wicri:Area/PubMed/Checkpoint">002628</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002628</idno><idno type="wicri:Area/Ncbi/Merge">002A07</idno><idno type="wicri:Area/Ncbi/Curation">002A07</idno><idno type="wicri:Area/Ncbi/Checkpoint">002A07</idno><idno type="wicri:doubleKey">0951-4198:1996:Boschenok J:electrospray:tandem:mass</idno><idno type="wicri:Area/Main/Merge">003E81</idno><idno type="wicri:Area/Main/Curation">003E26</idno><idno type="wicri:Area/Main/Exploration">003E26</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Electrospray tandem mass spectrometry of nucleotides.</title><author><name sortKey="Boschenok, J" sort="Boschenok, J" uniqKey="Boschenok J" first="J" last="Boschenok">J. Boschenok</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, University of Wollongong, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Chemistry, University of Wollongong, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author><author><name sortKey="Sheil, M M" sort="Sheil, M M" uniqKey="Sheil M" first="M M" last="Sheil">M M Sheil</name></author></analytic><series><title level="j">Rapid communications in mass spectrometry : RCM</title><idno type="ISSN">0951-4198</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Electrochemistry</term><term>Mass Spectrometry</term><term>Molecular Sequence Data</term><term>Oligonucleotides (analysis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Oligonucléotides (analyse)</term><term>Spectrométrie de masse</term><term>Séquence nucléotidique</term><term>Électrochimie</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Oligonucléotides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Electrochemistry</term><term>Mass Spectrometry</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Spectrométrie de masse</term><term>Séquence nucléotidique</term><term>Électrochimie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The tandem mass spectra (MS/MS) following electrospray ionization of nucleotides (from 2- to 6-mers) have been examined under a range of collision and ionization conditions. In general, abundant peaks due to the free bases and phosphate groups are observed, along with a limited number of sequence ions. A number of strategies for increasing the extent of the observed fragmentation were investigated. Increasing the skimmer potential of the ion source, thereby increasing the initial internal energy of the incident ions, did not significantly enhance the sequence-specific fragmentation, although the relative intensities of fragment ions increased. In MS/MS spectra of different adducts (i.e. [M + H]+, [M-H]-) of dinucleotides the intensity of the ions due to the bases (BH2+ or B-) is dependent on both the base position (i.e. either 3' or 5') and the nature of the adduct ion. These data show that MS/MS spectra of [M-H]- ions of dinucleotides generated by electrospray ionization may be equally as diagnostic as MS/MS spectra obtained following fast-atom bombardment. With [M + Li]+ ions, more sequence ions were observed compared to the corresponding protonated and deprotonated species. For oligonucleotide hexamers, MS/MS spectra of the [M-2H]2- ions show fragment ions due to loss of bases followed by cleavage of the 3' C--O bond in the sugar from which the base is lost (i.e. w ions); however, a strong preference for loss of any particular base is not observed. Finally, MS/MS spectra of 5'-ApCpC-3' and 5'-d(AGGCCT)-3' obtained on a magnetic sector orthogonal acceleration time-of-flight mass spectrometer demonstrate the potential utility of this instrumental configuration for MS/MS analysis of oligonucleotides.</div></front></TEI><affiliations><list><country><li>Australie</li></country></list><tree><noCountry><name sortKey="Sheil, M M" sort="Sheil, M M" uniqKey="Sheil M" first="M M" last="Sheil">M M Sheil</name></noCountry><country name="Australie"><noRegion><name sortKey="Boschenok, J" sort="Boschenok, J" uniqKey="Boschenok J" first="J" last="Boschenok">J. Boschenok</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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