Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air
Identifieur interne : 002563 ( Main/Exploration ); précédent : 002562; suivant : 002564Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air
Auteurs : Jing Lv [République populaire de Chine] ; Baozhi Wei [République populaire de Chine] ; Tongjie Chai [République populaire de Chine] ; Xianzhu Xia [République populaire de Chine] ; Zengmin Miao [République populaire de Chine] ; Meiling Yao [République populaire de Chine] ; Yuwei Gao [République populaire de Chine] ; Rong Huang [République populaire de Chine] ; Huili Yang [République populaire de Chine] ; Uwe Roesler [Allemagne]Source :
- Archives of Virology [ 0304-8608 ] ; 2011-10-01.
Abstract
Abstract: Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×104 to 6.92×104 copies/m3 air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.
Url:
DOI: 10.1007/s00705-011-1054-4
Affiliations:
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Prevention of Tai’an City, Tai’an</wicri:regionArea><wicri:noRegion>Tai’an</wicri:noRegion></affiliation></author><author><name sortKey="Roesler, Uwe" sort="Roesler, Uwe" uniqKey="Roesler U" first="Uwe" last="Roesler">Uwe Roesler</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Animal Hygiene and Environment Health, Free University Berlin, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="sub">Official Journal of the Virology Division of the International Union of Microbiological Societies</title><title level="j" type="abbrev">Arch Virol</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer Vienna</publisher><pubPlace>Vienna</pubPlace><date type="published" when="2011-10-01">2011-10-01</date><biblScope unit="volume">156</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="1795">1795</biblScope><biblScope unit="page" to="1801">1801</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×104 to 6.92×104 copies/m3 air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>République populaire de Chine</li></country><region><li>Berlin</li><li>Dongbei</li><li>Jilin</li></region><settlement><li>Berlin</li><li>Changchun</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Lv, Jing" sort="Lv, Jing" uniqKey="Lv J" first="Jing" last="Lv">Jing Lv</name></noRegion><name sortKey="Chai, Tongjie" sort="Chai, Tongjie" uniqKey="Chai T" first="Tongjie" last="Chai">Tongjie Chai</name><name sortKey="Chai, Tongjie" sort="Chai, Tongjie" uniqKey="Chai T" first="Tongjie" last="Chai">Tongjie Chai</name><name sortKey="Chai, Tongjie" sort="Chai, Tongjie" uniqKey="Chai T" first="Tongjie" last="Chai">Tongjie Chai</name><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name><name sortKey="Huang, Rong" sort="Huang, Rong" uniqKey="Huang R" first="Rong" last="Huang">Rong Huang</name><name sortKey="Huang, Rong" sort="Huang, Rong" uniqKey="Huang R" first="Rong" last="Huang">Rong Huang</name><name sortKey="Huang, Rong" sort="Huang, Rong" uniqKey="Huang R" first="Rong" last="Huang">Rong Huang</name><name sortKey="Lv, Jing" sort="Lv, Jing" uniqKey="Lv J" first="Jing" last="Lv">Jing Lv</name><name sortKey="Lv, Jing" sort="Lv, Jing" uniqKey="Lv J" first="Jing" last="Lv">Jing Lv</name><name sortKey="Miao, Zengmin" sort="Miao, Zengmin" uniqKey="Miao Z" first="Zengmin" last="Miao">Zengmin Miao</name><name sortKey="Miao, Zengmin" sort="Miao, Zengmin" uniqKey="Miao Z" first="Zengmin" last="Miao">Zengmin Miao</name><name sortKey="Miao, Zengmin" sort="Miao, Zengmin" uniqKey="Miao Z" first="Zengmin" last="Miao">Zengmin Miao</name><name sortKey="Wei, Baozhi" sort="Wei, Baozhi" uniqKey="Wei B" first="Baozhi" last="Wei">Baozhi Wei</name><name sortKey="Wei, Baozhi" sort="Wei, Baozhi" uniqKey="Wei B" first="Baozhi" last="Wei">Baozhi Wei</name><name sortKey="Wei, Baozhi" sort="Wei, Baozhi" uniqKey="Wei B" first="Baozhi" last="Wei">Baozhi Wei</name><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><name sortKey="Yang, Huili" sort="Yang, Huili" uniqKey="Yang H" first="Huili" last="Yang">Huili Yang</name><name sortKey="Yao, Meiling" sort="Yao, Meiling" uniqKey="Yao M" first="Meiling" last="Yao">Meiling Yao</name><name sortKey="Yao, Meiling" sort="Yao, Meiling" uniqKey="Yao M" first="Meiling" last="Yao">Meiling Yao</name><name sortKey="Yao, Meiling" sort="Yao, Meiling" uniqKey="Yao M" first="Meiling" last="Yao">Meiling Yao</name></country><country name="Allemagne"><region name="Berlin"><name sortKey="Roesler, Uwe" sort="Roesler, Uwe" uniqKey="Roesler U" first="Uwe" last="Roesler">Uwe Roesler</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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