Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air
Identifieur interne : 001176 ( Istex/Corpus ); précédent : 001175; suivant : 001177Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air
Auteurs : Jing Lv ; Baozhi Wei ; Tongjie Chai ; Xianzhu Xia ; Zengmin Miao ; Meiling Yao ; Yuwei Gao ; Rong Huang ; Huili Yang ; Uwe RoeslerSource :
- Archives of Virology [ 0304-8608 ] ; 2011-10-01.
Abstract
Abstract: Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×104 to 6.92×104 copies/m3 air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.
Url:
DOI: 10.1007/s00705-011-1054-4
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uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name><affiliation><mods:affiliation>Institute of Military Veterinary PLA, Changchun, China</mods:affiliation></affiliation></author><author><name sortKey="Huang, Rong" sort="Huang, Rong" uniqKey="Huang R" first="Rong" last="Huang">Rong Huang</name><affiliation><mods:affiliation>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</mods:affiliation></affiliation></author><author><name sortKey="Yang, Huili" sort="Yang, Huili" uniqKey="Yang H" first="Huili" last="Yang">Huili Yang</name><affiliation><mods:affiliation>Center for Disease Control and Prevention of Tai’an City, Tai’an, China</mods:affiliation></affiliation></author><author><name sortKey="Roesler, Uwe" sort="Roesler, Uwe" uniqKey="Roesler U" first="Uwe" last="Roesler">Uwe Roesler</name><affiliation><mods:affiliation>Institute of Animal Hygiene and Environment Health, Free University Berlin, Berlin, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="sub">Official Journal of the Virology Division of the International Union of Microbiological Societies</title><title level="j" type="abbrev">Arch Virol</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer Vienna</publisher><pubPlace>Vienna</pubPlace><date type="published" when="2011-10-01">2011-10-01</date><biblScope unit="volume">156</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="1795">1795</biblScope><biblScope unit="page" to="1801">1801</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×104 to 6.92×104 copies/m3 air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Jing Lv</name><affiliations><json:string>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</json:string><json:string>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</json:string><json:string>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</json:string></affiliations></json:item><json:item><name>Baozhi Wei</name><affiliations><json:string>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</json:string><json:string>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</json:string><json:string>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</json:string></affiliations></json:item><json:item><name>Tongjie Chai</name><affiliations><json:string>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</json:string><json:string>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</json:string><json:string>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</json:string><json:string>E-mail: chaitj117@163.com</json:string></affiliations></json:item><json:item><name>Xianzhu Xia</name><affiliations><json:string>Institute of Military Veterinary PLA, Changchun, China</json:string></affiliations></json:item><json:item><name>Zengmin Miao</name><affiliations><json:string>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</json:string><json:string>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</json:string><json:string>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</json:string></affiliations></json:item><json:item><name>Meiling Yao</name><affiliations><json:string>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</json:string><json:string>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</json:string><json:string>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</json:string></affiliations></json:item><json:item><name>Yuwei Gao</name><affiliations><json:string>Institute of Military Veterinary PLA, Changchun, China</json:string></affiliations></json:item><json:item><name>Rong Huang</name><affiliations><json:string>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</json:string><json:string>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</json:string><json:string>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</json:string></affiliations></json:item><json:item><name>Huili Yang</name><affiliations><json:string>Center for Disease Control and Prevention of Tai’an City, Tai’an, China</json:string></affiliations></json:item><json:item><name>Uwe Roesler</name><affiliations><json:string>Institute of Animal Hygiene and Environment Health, Free University Berlin, Berlin, Germany</json:string></affiliations></json:item></author><articleId><json:string>1054</json:string><json:string>s00705-011-1054-4</json:string></articleId><arkIstex>ark:/67375/VQC-52TBJFV5-0</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×104 to 6.92×104 copies/m3 air. 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xml:id="author-0006"><persName><forename type="first">Yuwei</forename><surname>Gao</surname></persName><affiliation>Institute of Military Veterinary PLA, Changchun, China</affiliation></author><author xml:id="author-0007"><persName><forename type="first">Rong</forename><surname>Huang</surname></persName><affiliation>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</affiliation><affiliation>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</affiliation><affiliation>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</affiliation></author><author xml:id="author-0008"><persName><forename type="first">Huili</forename><surname>Yang</surname></persName><affiliation>Center for Disease Control and Prevention of Tai’an City, Tai’an, China</affiliation></author><author 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type="published" when="2011-10-01"></date><biblScope unit="volume">156</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="1795">1795</biblScope><biblScope unit="page" to="1801">1801</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2011-04-07</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×104 to 6.92×104 copies/m3 air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Virology</term></item><item><term>Infectious Diseases</term></item><item><term>Medical Microbiology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2011-04-07">Created</change><change when="2011-10-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-52TBJFV5-0/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer Vienna</PublisherName><PublisherLocation>Vienna</PublisherLocation></PublisherInfo><Journal OutputMedium="All"><JournalInfo JournalProductType="NonStandardArchiveJournal" NumberingStyle="Unnumbered"><JournalID>705</JournalID><JournalPrintISSN>0304-8608</JournalPrintISSN><JournalElectronicISSN>1432-8798</JournalElectronicISSN><JournalTitle>Archives of Virology</JournalTitle><JournalSubTitle>Official Journal of the Virology Division of the International Union of Microbiological Societies</JournalSubTitle><JournalAbbreviatedTitle>Arch Virol</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Biomedicine</JournalSubject><JournalSubject Type="Secondary">Virology</JournalSubject><JournalSubject Type="Secondary">Infectious Diseases</JournalSubject><JournalSubject Type="Secondary">Medical Microbiology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume OutputMedium="All"><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>156</VolumeIDStart><VolumeIDEnd>156</VolumeIDEnd><VolumeIssueCount>12</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular" OutputMedium="All"><IssueInfo IssueType="Regular" TocLevels="0"><IssueIDStart>10</IssueIDStart><IssueIDEnd>10</IssueIDEnd><IssueArticleCount>29</IssueArticleCount><IssueHistory><OnlineDate><Year>2011</Year><Month>9</Month><Day>28</Day></OnlineDate><PrintDate><Year>2011</Year><Month>9</Month><Day>27</Day></PrintDate><CoverDate><Year>2011</Year><Month>10</Month></CoverDate><PricelistYear>2011</PricelistYear></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>2011</CopyrightYear></IssueCopyright></IssueInfo><Article ID="s00705-011-1054-4" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>1054</ArticleID><ArticleDOI>10.1007/s00705-011-1054-4</ArticleDOI><ArticleCitationID>1795</ArticleCitationID><ArticleSequenceNumber>10</ArticleSequenceNumber><ArticleTitle Language="En" OutputMedium="All">Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air</ArticleTitle><ArticleCategory>Original Article</ArticleCategory><ArticleFirstPage>1795</ArticleFirstPage><ArticleLastPage>1801</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2011</Year><Month>6</Month><Day>18</Day></RegistrationDate><Received><Year>2011</Year><Month>4</Month><Day>7</Day></Received><Accepted><Year>2011</Year><Month>6</Month><Day>18</Day></Accepted><OnlineDate><Year>2011</Year><Month>7</Month><Day>7</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>2011</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2 Aff3"><AuthorName DisplayOrder="Western"><GivenName>Jing</GivenName><FamilyName>Lv</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2 Aff3"><AuthorName DisplayOrder="Western"><GivenName>Baozhi</GivenName><FamilyName>Wei</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2 Aff3" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Tongjie</GivenName><FamilyName>Chai</FamilyName></AuthorName><Contact><Phone>+86-538-8249951</Phone><Fax>+86-538-8241503</Fax><Email>chaitj117@163.com</Email></Contact></Author><Author AffiliationIDS="Aff4"><AuthorName DisplayOrder="Western"><GivenName>Xianzhu</GivenName><FamilyName>Xia</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2 Aff3"><AuthorName DisplayOrder="Western"><GivenName>Zengmin</GivenName><FamilyName>Miao</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2 Aff3"><AuthorName DisplayOrder="Western"><GivenName>Meiling</GivenName><FamilyName>Yao</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff4"><AuthorName DisplayOrder="Western"><GivenName>Yuwei</GivenName><FamilyName>Gao</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2 Aff3"><AuthorName DisplayOrder="Western"><GivenName>Rong</GivenName><FamilyName>Huang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff5"><AuthorName DisplayOrder="Western"><GivenName>Huili</GivenName><FamilyName>Yang</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff6"><AuthorName DisplayOrder="Western"><GivenName>Uwe</GivenName><FamilyName>Roesler</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>College of Animal Science and Veterinary Medicine</OrgDivision><OrgName>Shandong Agricultural University</OrgName><OrgAddress><Street>Daizong Street 61</Street><City>Tai’an</City><Postcode>271018</Postcode><State>Shandong</State><Country Code="CN">China</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgName>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province</OrgName><OrgAddress><City>Tai’an</City><State>Shandong</State><Country Code="CN">China</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgName>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province</OrgName><OrgAddress><City>Tai’an</City><State>Shandong</State><Country Code="CN">China</Country></OrgAddress></Affiliation><Affiliation ID="Aff4"><OrgName>Institute of Military Veterinary PLA</OrgName><OrgAddress><City>Changchun</City><Country Code="CN">China</Country></OrgAddress></Affiliation><Affiliation ID="Aff5"><OrgName>Center for Disease Control and Prevention of Tai’an City</OrgName><OrgAddress><City>Tai’an</City><Country Code="CN">China</Country></OrgAddress></Affiliation><Affiliation ID="Aff6"><OrgDivision>Institute of Animal Hygiene and Environment Health</OrgDivision><OrgName>Free University Berlin</OrgName><OrgAddress><City>Berlin</City><Country Code="DE">Germany</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para TextBreak="No">Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×10<Superscript>4</Superscript> to 6.92×10<Superscript>4</Superscript> copies/m<Superscript>3</Superscript> air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.</Para></Abstract></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air</title></titleInfo><name type="personal"><namePart type="given">Jing</namePart><namePart type="family">Lv</namePart><affiliation>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</affiliation><affiliation>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</affiliation><affiliation>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Baozhi</namePart><namePart type="family">Wei</namePart><affiliation>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</affiliation><affiliation>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</affiliation><affiliation>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Tongjie</namePart><namePart type="family">Chai</namePart><affiliation>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</affiliation><affiliation>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</affiliation><affiliation>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</affiliation><affiliation>E-mail: chaitj117@163.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Xianzhu</namePart><namePart type="family">Xia</namePart><affiliation>Institute of Military Veterinary PLA, Changchun, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Zengmin</namePart><namePart type="family">Miao</namePart><affiliation>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</affiliation><affiliation>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</affiliation><affiliation>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Meiling</namePart><namePart type="family">Yao</namePart><affiliation>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</affiliation><affiliation>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</affiliation><affiliation>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yuwei</namePart><namePart type="family">Gao</namePart><affiliation>Institute of Military Veterinary PLA, Changchun, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rong</namePart><namePart type="family">Huang</namePart><affiliation>College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, 271018, Tai’an, Shandong, China</affiliation><affiliation>Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China</affiliation><affiliation>Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Huili</namePart><namePart type="family">Yang</namePart><affiliation>Center for Disease Control and Prevention of Tai’an City, Tai’an, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Uwe</namePart><namePart type="family">Roesler</namePart><affiliation>Institute of Animal Hygiene and Environment Health, Free University Berlin, Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Vienna</publisher><place><placeTerm type="text">Vienna</placeTerm></place><dateCreated encoding="w3cdtf">2011-04-07</dateCreated><dateIssued encoding="w3cdtf">2011-10-01</dateIssued><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×104 to 6.92×104 copies/m3 air. 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