Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air
Identifieur interne : 001176 ( Istex/Curation ); précédent : 001175; suivant : 001177Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air
Auteurs : Jing Lv [République populaire de Chine] ; Baozhi Wei [République populaire de Chine] ; Tongjie Chai [République populaire de Chine] ; Xianzhu Xia [République populaire de Chine] ; Zengmin Miao [République populaire de Chine] ; Meiling Yao [République populaire de Chine] ; Yuwei Gao [République populaire de Chine] ; Rong Huang [République populaire de Chine] ; Huili Yang [République populaire de Chine] ; Uwe Roesler [Allemagne]Source :
- Archives of Virology [ 0304-8608 ] ; 2011-10-01.
Abstract
Abstract: Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×104 to 6.92×104 copies/m3 air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.
Url:
DOI: 10.1007/s00705-011-1054-4
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<front><div type="abstract" xml:lang="en">Abstract: Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×104 to 6.92×104 copies/m3 air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.</div>
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