Studying Evolutionary Adaptation of MERS-CoV
Identifieur interne : 000363 ( Main/Exploration ); précédent : 000362; suivant : 000364Studying Evolutionary Adaptation of MERS-CoV
Auteurs :Source :
- MERS Coronavirus ; 2019.
Abstract
Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730–1737, 2018.
Url:
DOI: 10.1007/978-1-0716-0211-9_1
PubMed: 31883083
PubMed Central: 7121928
Affiliations:
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