MersV1, PubMed, Corpus, bibRecord, 000317

Studying Evolutionary Adaptation of MERS-CoV.

Identifieur interne : 000317 ( PubMed/Corpus ); précédent : 000316; suivant : 000318

Studying Evolutionary Adaptation of MERS-CoV.

Auteurs : Michael Letko ; Vincent Munster

Source :

Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2020.

RBID : pubmed:31883083

Abstract

Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730-1737, 2018.

DOI: 10.1007/978-1-0716-0211-9_1
PubMed: 31883083

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pubmed:31883083

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<author><name sortKey="Letko, Michael" sort="Letko, Michael" uniqKey="Letko M" first="Michael" last="Letko">Michael Letko</name>
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<author><name sortKey="Munster, Vincent" sort="Munster, Vincent" uniqKey="Munster V" first="Vincent" last="Munster">Vincent Munster</name>
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<front><div type="abstract" xml:lang="en">Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730-1737, 2018.</div>
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<Keyword MajorTopicYN="Y">Host restriction</Keyword>
<Keyword MajorTopicYN="Y">MERS-CoV</Keyword>
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Studying Evolutionary Adaptation of MERS-CoV.

Studying Evolutionary Adaptation of MERS-CoV.

Source :

Abstract

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