MersV1, Pmc, Checkpoint, bibRecord, 000167

Studying Evolutionary Adaptation of MERS-CoV

Identifieur interne : 000167 ( Pmc/Checkpoint ); précédent : 000166; suivant : 000168

Studying Evolutionary Adaptation of MERS-CoV

Auteurs :

Source :

MERS Coronavirus ; 2019.

RBID : PMC:7121928

Abstract

Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730–1737, 2018.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121928

DOI: 10.1007/978-1-0716-0211-9_1
PubMed: 31883083
PubMed Central: 7121928

Affiliations:

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PMC:7121928

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p id="Par1">Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730–1737, 2018.</p>
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<contrib-group content-type="book editors"><contrib contrib-type="editor"><name><surname>Vijay</surname>
<given-names>Rahul</given-names>
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<address><email>rahul-vijay@uiowa.edu</email>
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<institution>Department of Microbiology and Immunology,</institution>
<institution>University of Iowa,</institution>
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Iowa City, IA USA</aff>
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<contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Letko</surname>
<given-names>Michael</given-names>
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<address><email>michael.letko@nih.gov</email>
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<contrib contrib-type="author"><name><surname>Munster</surname>
<given-names>Vincent</given-names>
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<institution>Laboratory of Virology, Division of Intramural Research,</institution>
<institution>National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institutes of Health,</institution>
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Hamilton, MT USA</aff>
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<pub-date pub-type="epub"><day>14</day>
<month>9</month>
<year>2019</year>
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<pub-date pub-type="collection"><year>2020</year>
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<volume>2099</volume>
<fpage>3</fpage>
<lpage>8</lpage>
<permissions><copyright-statement>© Springer Science+Business Media, LLC, part of Springer Nature 2020</copyright-statement>
<license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p>
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<abstract id="Abs1"><p id="Par1">Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730–1737, 2018.</p>
</abstract>
<kwd-group xml:lang="en"><title>Key words</title>
<kwd>MERS-CoV</kwd>
<kwd>Forced adaptation</kwd>
<kwd>Experimental evolution</kwd>
<kwd>Cell culture</kwd>
<kwd>Semi-permissive cell line</kwd>
<kwd>Host restriction</kwd>
<kwd>Species barrier</kwd>
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Studying Evolutionary Adaptation of MERS-CoV

Studying Evolutionary Adaptation of MERS-CoV

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Abstract

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