Overexpression of truncated Nmd3p inhibits protein synthesis in yeast.
Identifieur interne : 003792 ( Main/Curation ); précédent : 003791; suivant : 003793Overexpression of truncated Nmd3p inhibits protein synthesis in yeast.
Auteurs : J P Belk [États-Unis] ; F. He ; A. JacobsonSource :
- RNA (New York, N.Y.) [ 1355-8382 ] ; 1999.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), ARN ribosomique (génétique), ARN ribosomique (métabolisme), Biosynthèse des protéines, Clonage moléculaire, Division cellulaire (génétique), Données de séquences moléculaires, Facteurs temps, Galactose (pharmacologie), Gènes dominants, Inhibiteurs de la synthèse protéique (métabolisme), Modèles génétiques, Polyribosomes (métabolisme), Protéines de Saccharomyces cerevisiae, Protéines de liaison à l'ARN, Protéines fongiques (génétique), Protéines fongiques (métabolisme), Protéines fongiques (physiologie), RNA helicases (métabolisme), Régulation de l'expression des gènes fongiques, Saccharomyces cerevisiae (métabolisme), Similitude de séquences d'acides aminés, Séquence d'acides aminés, Technique de Northern.
- MESH :
- génétique : ARN ribosomique, Division cellulaire, Protéines fongiques.
- métabolisme : ARN messager, ARN ribosomique, Inhibiteurs de la synthèse protéique, Polyribosomes, Protéines fongiques, RNA helicases, Saccharomyces cerevisiae.
- pharmacologie : Galactose.
- physiologie : Protéines fongiques.
- Biosynthèse des protéines, Clonage moléculaire, Données de séquences moléculaires, Facteurs temps, Gènes dominants, Modèles génétiques, Protéines de Saccharomyces cerevisiae, Protéines de liaison à l'ARN, Régulation de l'expression des gènes fongiques, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Technique de Northern.
English descriptors
- KwdEn :
- Amino Acid Sequence, Blotting, Northern, Cell Division (genetics), Cloning, Molecular, Fungal Proteins (genetics), Fungal Proteins (metabolism), Fungal Proteins (physiology), Galactose (pharmacology), Gene Expression Regulation, Fungal, Genes, Dominant, Models, Genetic, Molecular Sequence Data, Polyribosomes (metabolism), Protein Biosynthesis, Protein Synthesis Inhibitors (metabolism), RNA Helicases (metabolism), RNA, Messenger (metabolism), RNA, Ribosomal (genetics), RNA, Ribosomal (metabolism), RNA-Binding Proteins, Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Time Factors.
- MESH :
- chemical , genetics : Fungal Proteins, RNA, Ribosomal.
- genetics : Cell Division.
- chemical , metabolism : Fungal Proteins, Polyribosomes, Protein Synthesis Inhibitors, RNA Helicases, RNA, Messenger, RNA, Ribosomal, Saccharomyces cerevisiae.
- chemical , pharmacology : Galactose.
- chemical , physiology : Fungal Proteins.
- Amino Acid Sequence, Blotting, Northern, Cloning, Molecular, Gene Expression Regulation, Fungal, Genes, Dominant, Models, Genetic, Molecular Sequence Data, Protein Biosynthesis, RNA-Binding Proteins, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Time Factors.
Abstract
The yeast NMD3 gene was identified in a two-hybrid screen using the nonsense-mediated mRNA decay factor, Upf1p, as bait. NMD3 was shown to encode an essential, highly conserved protein that associated principally with free 60S ribosomal subunits. Overexpression of a truncated form of Nmd3p, lacking 100 C-terminal amino acids and most of its Upf1p-interacting domain, had dominant-negative effects on both cell growth and protein synthesis and promoted the formation of polyribosome half-mers. These effects were eliminated by truncation of an additional 100 amino acids from Nmd3p. Overexpression of the nmd3delta100 allele also led to increased synthesis and destabilization of some ribosomal protein mRNAs, and increased synthesis and altered processing of 35S pre-rRNA. Our data suggest that Nmd3p has a role in the formation, function, or maintenance of the 60S ribosomal subunit and may provide a link for Upf1p to 80S monosomes.
DOI: 10.1017/s1355838299990027
PubMed: 10445880
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pubmed:10445880Le document en format XML
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<term>Blotting, Northern</term>
<term>Cell Division (genetics)</term>
<term>Cloning, Molecular</term>
<term>Fungal Proteins (genetics)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Fungal Proteins (physiology)</term>
<term>Galactose (pharmacology)</term>
<term>Gene Expression Regulation, Fungal</term>
<term>Genes, Dominant</term>
<term>Models, Genetic</term>
<term>Molecular Sequence Data</term>
<term>Polyribosomes (metabolism)</term>
<term>Protein Biosynthesis</term>
<term>Protein Synthesis Inhibitors (metabolism)</term>
<term>RNA Helicases (metabolism)</term>
<term>RNA, Messenger (metabolism)</term>
<term>RNA, Ribosomal (genetics)</term>
<term>RNA, Ribosomal (metabolism)</term>
<term>RNA-Binding Proteins</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Sequence Homology, Amino Acid</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (métabolisme)</term>
<term>ARN ribosomique (génétique)</term>
<term>ARN ribosomique (métabolisme)</term>
<term>Biosynthèse des protéines</term>
<term>Clonage moléculaire</term>
<term>Division cellulaire (génétique)</term>
<term>Données de séquences moléculaires</term>
<term>Facteurs temps</term>
<term>Galactose (pharmacologie)</term>
<term>Gènes dominants</term>
<term>Inhibiteurs de la synthèse protéique (métabolisme)</term>
<term>Modèles génétiques</term>
<term>Polyribosomes (métabolisme)</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Protéines de liaison à l'ARN</term>
<term>Protéines fongiques (génétique)</term>
<term>Protéines fongiques (métabolisme)</term>
<term>Protéines fongiques (physiologie)</term>
<term>RNA helicases (métabolisme)</term>
<term>Régulation de l'expression des gènes fongiques</term>
<term>Saccharomyces cerevisiae (métabolisme)</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Séquence d'acides aminés</term>
<term>Technique de Northern</term>
</keywords>
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<term>RNA, Ribosomal</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Division</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN ribosomique</term>
<term>Division cellulaire</term>
<term>Protéines fongiques</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term>
<term>Polyribosomes</term>
<term>Protein Synthesis Inhibitors</term>
<term>RNA Helicases</term>
<term>RNA, Messenger</term>
<term>RNA, Ribosomal</term>
<term>Saccharomyces cerevisiae</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term>
<term>ARN ribosomique</term>
<term>Inhibiteurs de la synthèse protéique</term>
<term>Polyribosomes</term>
<term>Protéines fongiques</term>
<term>RNA helicases</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Galactose</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Galactose</term>
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<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Protéines fongiques</term>
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<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Fungal Proteins</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Blotting, Northern</term>
<term>Cloning, Molecular</term>
<term>Gene Expression Regulation, Fungal</term>
<term>Genes, Dominant</term>
<term>Models, Genetic</term>
<term>Molecular Sequence Data</term>
<term>Protein Biosynthesis</term>
<term>RNA-Binding Proteins</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Sequence Homology, Amino Acid</term>
<term>Time Factors</term>
</keywords>
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<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Facteurs temps</term>
<term>Gènes dominants</term>
<term>Modèles génétiques</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Protéines de liaison à l'ARN</term>
<term>Régulation de l'expression des gènes fongiques</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Séquence d'acides aminés</term>
<term>Technique de Northern</term>
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<front><div type="abstract" xml:lang="en">The yeast NMD3 gene was identified in a two-hybrid screen using the nonsense-mediated mRNA decay factor, Upf1p, as bait. NMD3 was shown to encode an essential, highly conserved protein that associated principally with free 60S ribosomal subunits. Overexpression of a truncated form of Nmd3p, lacking 100 C-terminal amino acids and most of its Upf1p-interacting domain, had dominant-negative effects on both cell growth and protein synthesis and promoted the formation of polyribosome half-mers. These effects were eliminated by truncation of an additional 100 amino acids from Nmd3p. Overexpression of the nmd3delta100 allele also led to increased synthesis and destabilization of some ribosomal protein mRNAs, and increased synthesis and altered processing of 35S pre-rRNA. Our data suggest that Nmd3p has a role in the formation, function, or maintenance of the 60S ribosomal subunit and may provide a link for Upf1p to 80S monosomes.</div>
</front>
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