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High-Resolution Comparative Mapping of Mouse Chromosome 17

Identifieur interne : 002710 ( Istex/Curation ); précédent : 002709; suivant : 002711

High-Resolution Comparative Mapping of Mouse Chromosome 17

Auteurs : Heinz Himmelbauer [États-Unis] ; Lee M. Silver [États-Unis]

Source :

RBID : ISTEX:3AB6C8D9E6F4CAF4CD19C6D75A1C45E41A78FC17

Abstract

Abstract: We have constructed a high-resolution genetic linkage map spanning the proximal 32 cM of mouse chromosome 17 including the t complex. Simple sequence repeats at D17Tu1 and D17Mit6 were employed to identify 121 recombinants among 374 offspring of a (C57BL/6) × CAST/Ei) × C57BL/6 backcross. In contrast to previously reported interspecific Mus domesticus × Mus spretus backcrosses, we did not observe inversion polymorphisms with our cross. This and the relatively high frequency of DNA polymorphisms between C57BL/6 and CAST/Ei allowed us to put 32 RFLV markers and 32 PCR markers on a single map. We present the localization of four new DNA markers and determined map positions for 10 other loci, which previously had been assigned to intervals of the t complex only through the study of partial t haplotype chromosomes.

Url:
DOI: 10.1006/geno.1993.1291

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ISTEX:3AB6C8D9E6F4CAF4CD19C6D75A1C45E41A78FC17

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<div type="abstract" xml:lang="en">Abstract: We have constructed a high-resolution genetic linkage map spanning the proximal 32 cM of mouse chromosome 17 including the t complex. Simple sequence repeats at D17Tu1 and D17Mit6 were employed to identify 121 recombinants among 374 offspring of a (C57BL/6) × CAST/Ei) × C57BL/6 backcross. In contrast to previously reported interspecific Mus domesticus × Mus spretus backcrosses, we did not observe inversion polymorphisms with our cross. This and the relatively high frequency of DNA polymorphisms between C57BL/6 and CAST/Ei allowed us to put 32 RFLV markers and 32 PCR markers on a single map. We present the localization of four new DNA markers and determined map positions for 10 other loci, which previously had been assigned to intervals of the t complex only through the study of partial t haplotype chromosomes.</div>
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