Solution Structure of a Parallel-stranded Tetraplex Formed by d(TG4T) in the Presence of Sodium Ions by Nuclear Magnetic Resonance Spectroscopy
Identifieur interne : 002709 ( Istex/Curation ); précédent : 002708; suivant : 002710Solution Structure of a Parallel-stranded Tetraplex Formed by d(TG4T) in the Presence of Sodium Ions by Nuclear Magnetic Resonance Spectroscopy
Auteurs : Fareed Aboul-Ela [États-Unis] ; Alastair I. H. Murchie [États-Unis] ; David G. Norman [États-Unis] ; David M. J. Lilley [États-Unis]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 1994.
English descriptors
- KwdEn :
Abstract
Abstract: DNA sequences containing repetitive oligo-guanine sequences are observed in telomeric DNA and in recombination hotspots. In vitro, these sequences fold into at least two classes of unusual conformations involving the formation of guanine tetrads (G-quartets). Through the use of NMR, we have established that in solution in the presence of sodium ions, the oligodeoxynucleotide dTG4T forms a parallel-stranded tatraplex, with hydrogen-bonded guanine tetrads. Here we present the detailed evidence for this configuration in 100 mM sodium ions at neutral pH. We show that the parallel-stranded tetraplex structure is stabilised relative to the single-strands when Na ions are replaced by K ions, without any evidence of significant change in those chemical shifts associated with the tetraplex form. We also present a model for the Na + solution structure of the tetraplex, by back-calculation from NOE volumes. We obtain excellent agreement with the data for structures with characteristics similar to B-DNA for the internal tetrads, G3 and G4. The outer tetrads, particularly the 5′-terminal G2, display some unstacking. We see no evidence for formation of thymine tetrads analogous to the uridine tetrads observed in RNA, but rather we find the terminal thymine conformations are not well determined by our data. We observe an uninterrupted cylindrical channel through the centre of the complex.
Url:
DOI: 10.1006/jmbi.1994.1672
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002709
Links to Exploration step
ISTEX:41E7AF221D07078112350721EDC658D8D9600C58Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Solution Structure of a Parallel-stranded Tetraplex Formed by d(TG4T) in the Presence of Sodium Ions by Nuclear Magnetic Resonance Spectroscopy</title><author><name sortKey="Aboul Ela, Fareed" sort="Aboul Ela, Fareed" uniqKey="Aboul Ela F" first="Fareed" last="Aboul-Ela">Fareed Aboul-Ela</name><affiliation wicri:level="1"><mods:affiliation>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215</wicri:regionArea></affiliation></author><author><name sortKey="Murchie, Alastair I H" sort="Murchie, Alastair I H" uniqKey="Murchie A" first="Alastair I. H." last="Murchie">Alastair I. H. Murchie</name><affiliation wicri:level="1"><mods:affiliation>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215</wicri:regionArea></affiliation></author><author><name sortKey="Norman, David G" sort="Norman, David G" uniqKey="Norman D" first="David G." last="Norman">David G. Norman</name><affiliation wicri:level="1"><mods:affiliation>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215</wicri:regionArea></affiliation></author><author><name sortKey="Lilley, David M J" sort="Lilley, David M J" uniqKey="Lilley D" first="David M. J." last="Lilley">David M. J. Lilley</name><affiliation wicri:level="1"><mods:affiliation>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:41E7AF221D07078112350721EDC658D8D9600C58</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1006/jmbi.1994.1672</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-QCGSXV0W-P/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002709</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002709</idno><idno type="wicri:Area/Istex/Curation">002709</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Solution Structure of a Parallel-stranded Tetraplex Formed by d(TG4T) in the Presence of Sodium Ions by Nuclear Magnetic Resonance Spectroscopy</title><author><name sortKey="Aboul Ela, Fareed" sort="Aboul Ela, Fareed" uniqKey="Aboul Ela F" first="Fareed" last="Aboul-Ela">Fareed Aboul-Ela</name><affiliation wicri:level="1"><mods:affiliation>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215</wicri:regionArea></affiliation></author><author><name sortKey="Murchie, Alastair I H" sort="Murchie, Alastair I H" uniqKey="Murchie A" first="Alastair I. H." last="Murchie">Alastair I. H. Murchie</name><affiliation wicri:level="1"><mods:affiliation>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215</wicri:regionArea></affiliation></author><author><name sortKey="Norman, David G" sort="Norman, David G" uniqKey="Norman D" first="David G." last="Norman">David G. Norman</name><affiliation wicri:level="1"><mods:affiliation>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215</wicri:regionArea></affiliation></author><author><name sortKey="Lilley, David M J" sort="Lilley, David M J" uniqKey="Lilley D" first="David M. J." last="Lilley">David M. J. Lilley</name><affiliation wicri:level="1"><mods:affiliation>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>CRC Nucleic Acid Structure Research Group Department of Biochemistry, The University, Dundee DD1 4HN, U.K. and Department of Chemistry & Biochemistry University of Colorado at Boulder, Boulder, Colorado 80309-0215</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Molecular Biology</title><title level="j" type="abbrev">YJMBI</title><idno type="ISSN">0022-2836</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">243</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="458">458</biblScope><biblScope unit="page" to="471">471</biblScope></imprint><idno type="ISSN">0022-2836</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-2836</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA structure</term><term>G-quartet</term><term>NMR</term><term>telomeres</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: DNA sequences containing repetitive oligo-guanine sequences are observed in telomeric DNA and in recombination hotspots. In vitro, these sequences fold into at least two classes of unusual conformations involving the formation of guanine tetrads (G-quartets). Through the use of NMR, we have established that in solution in the presence of sodium ions, the oligodeoxynucleotide dTG4T forms a parallel-stranded tatraplex, with hydrogen-bonded guanine tetrads. Here we present the detailed evidence for this configuration in 100 mM sodium ions at neutral pH. We show that the parallel-stranded tetraplex structure is stabilised relative to the single-strands when Na ions are replaced by K ions, without any evidence of significant change in those chemical shifts associated with the tetraplex form. We also present a model for the Na + solution structure of the tetraplex, by back-calculation from NOE volumes. We obtain excellent agreement with the data for structures with characteristics similar to B-DNA for the internal tetrads, G3 and G4. The outer tetrads, particularly the 5′-terminal G2, display some unstacking. We see no evidence for formation of thymine tetrads analogous to the uridine tetrads observed in RNA, but rather we find the terminal thymine conformations are not well determined by our data. We observe an uninterrupted cylindrical channel through the centre of the complex.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002709 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 002709 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:41E7AF221D07078112350721EDC658D8D9600C58
|texte= Solution Structure of a Parallel-stranded Tetraplex Formed by d(TG4T) in the Presence of Sodium Ions by Nuclear Magnetic Resonance Spectroscopy
}}
| This area was generated with Dilib version V0.6.33. |  |