High-Resolution Comparative Mapping of Mouse Chromosome 17
Identifieur interne : 002710 ( Istex/Corpus ); précédent : 002709; suivant : 002711High-Resolution Comparative Mapping of Mouse Chromosome 17
Auteurs : Heinz Himmelbauer ; Lee M. SilverSource :
- Genomics [ 0888-7543 ] ; 1993.
Abstract
Abstract: We have constructed a high-resolution genetic linkage map spanning the proximal 32 cM of mouse chromosome 17 including the t complex. Simple sequence repeats at D17Tu1 and D17Mit6 were employed to identify 121 recombinants among 374 offspring of a (C57BL/6) × CAST/Ei) × C57BL/6 backcross. In contrast to previously reported interspecific Mus domesticus × Mus spretus backcrosses, we did not observe inversion polymorphisms with our cross. This and the relatively high frequency of DNA polymorphisms between C57BL/6 and CAST/Ei allowed us to put 32 RFLV markers and 32 PCR markers on a single map. We present the localization of four new DNA markers and determined map positions for 10 other loci, which previously had been assigned to intervals of the t complex only through the study of partial t haplotype chromosomes.
Url:
DOI: 10.1006/geno.1993.1291
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Simple sequence repeats at <ce:italic>D17Tu1</ce:italic> and <ce:italic>D17Mit6</ce:italic> were employed to identify 121 recombinants among 374 offspring of a (C57BL/6) × CAST/Ei) × C57BL/6 backcross. In contrast to previously reported interspecific <ce:italic>Mus domesticus</ce:italic> × <ce:italic>Mus spretus</ce:italic> backcrosses, we did not observe inversion polymorphisms with our cross. This and the relatively high frequency of DNA polymorphisms between C57BL/6 and CAST/Ei allowed us to put 32 RFLV markers and 32 PCR markers on a single map. We present the localization of four new DNA markers and determined map positions for 10 other loci, which previously had been assigned to intervals of the <ce:italic>t</ce:italic> complex only through the study of partial <ce:italic>t</ce:italic> haplotype chromosomes.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>High-Resolution Comparative Mapping of Mouse Chromosome 17</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>High-Resolution Comparative Mapping of Mouse Chromosome 17</title></titleInfo><name type="personal"><namePart type="given">Heinz</namePart><namePart type="family">Himmelbauer</namePart><affiliation>Department of Molecular Biology, Princeton University, New Jersey 08544 1014</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lee M.</namePart><namePart type="family">Silver</namePart><affiliation>Department of Molecular Biology, Princeton University, New Jersey 08544 1014</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: We have constructed a high-resolution genetic linkage map spanning the proximal 32 cM of mouse chromosome 17 including the t complex. Simple sequence repeats at D17Tu1 and D17Mit6 were employed to identify 121 recombinants among 374 offspring of a (C57BL/6) × CAST/Ei) × C57BL/6 backcross. In contrast to previously reported interspecific Mus domesticus × Mus spretus backcrosses, we did not observe inversion polymorphisms with our cross. This and the relatively high frequency of DNA polymorphisms between C57BL/6 and CAST/Ei allowed us to put 32 RFLV markers and 32 PCR markers on a single map. 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