Detection of HBV DNA in non-A, non-B hepatic tissues using the polymerase chain reaction assay
Identifieur interne : 000991 ( Istex/Curation ); précédent : 000990; suivant : 000992Detection of HBV DNA in non-A, non-B hepatic tissues using the polymerase chain reaction assay
Auteurs : Showgo Ohkoshi [Japon]Source :
- Gastroenterologia Japonica [ 0435-1339 ] ; 1991-12-01.
English descriptors
- KwdEn :
- Teeft :
- Alcoholic liver disease, Author thanks, Carcinoma, Causative agent, Chronic hepatitis, Chronic liver disease, Core antigen, Enzymatic amplification, Hbcag region, Hbsag, Hbsag region, Hepatic tissue, Hepatic tissues, Hepatitis, Hepatocellular, Hepatocellular carcinoma, Human hepatitis, Hybridization, Kodak film, Liver disease, Liver specimens, Liver tissue, Normal liver, Polymerase, Polymerase chain reaction, Posttransfusion hepatitis, Primers hbcp1, Primers hbsp1, Proc natl acad, Reaction mixture, Several reports, Southern blot analysis, Southern blot hybridization, Surface antigen, Surgical resection.
Abstract
Summary: The polymerase chain reaction (PCR) followed by Southern blotting was used to examine the presence of hepatitis B virus (HBV) DNA in non-cancerous liver tissue specimens from 22 Japanese hepatocellular carcinoma (HCC) patients, who were negative for HBV surface antigen (HBsAg). By Southern blot analysis, HBV DNA was negative in all 22 patients, but it was detected by the PCR in 8 of the 15 patients who were positive for antibodies against HBsAg or HBV core antigen. Seven patients who were negative for those antibodies were also negative for HBV DNA by the PCR. These results suggest that HBV may be involved in the etiology of the liver disease of some patients with what is presently classified as non-A, non-B hepatitis, if they are positive for HBV antibodies.
Url:
DOI: 10.1007/BF02782860
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