Incomplete replication of human parainfluenza virus type 4 in LLC-MK2 cells and in L929 cells
Identifieur interne : 000990 ( Istex/Curation ); précédent : 000989; suivant : 000991Incomplete replication of human parainfluenza virus type 4 in LLC-MK2 cells and in L929 cells
Auteurs : Hiroshi Komada [Japon] ; Hisae Inoue [Japon] ; Chihiro Yamabayashi [Japon] ; Morihiro Ito [Japon] ; Mitsuo Kawano [Japon] ; Machiko Nishio [Japon] ; Masato Tsurudome [Japon] ; Yuji Kozuka [Japon] ; Naoya Noda [Japon] ; Kazuyoshi Namba [Japon] ; Myles O'Brien [Japon] ; Yasuhiko Ito [Japon]Source :
- Medical Microbiology and Immunology [ 0300-8584 ] ; 2000-06-01.
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Abstract
Abstract: Human parainfluenza virus type 4A (hPIV-4A) and type 4B (hPIV-4B) were tested for their ability to replicate in the monkey kidney LLC-MK2 cell line (MK2 cells) and the murine L929 cell line (L929 cells). These cells are normally non-permissive for replication of hPIV-4; however, treatment with acetylated trypsin led to virus replication in MK2 cells, but was less effective for L929 cells. Endogenously produced interferon (IFN) played no role in virus replication in L929 cells. Synthesis of virus-specific polypeptides was suppressed in L929 cells. WhereasNP-mRNA and HN-mRNA were detected in MK2 cells, no HN-mRNA was detected in L929 cells. These results indicate that hPIV-4 can infect both MK2 cells and L929 cells. In MK2 cells, when protease exists in the extracellular medium, hPIV-4 exhibits multistep growth. In L929 cells, however, the cause of incomplete replication might be lack of other unknown factors.
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DOI: 10.1007/s004300000037
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sort="O Brien, Myles" uniqKey="O Brien M" first="Myles" last="O'Brien">Myles O'Brien</name><affiliation wicri:level="1"><mods:affiliation>Mie Prefectural College of Nursing, 514-0116, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Mie Prefectural College of Nursing, 514-0116, Tsu, Mie</wicri:regionArea></affiliation></author><author><name sortKey="Ito, Yasuhiko" sort="Ito, Yasuhiko" uniqKey="Ito Y" first="Yasuhiko" last="Ito">Yasuhiko Ito</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: Ito@doc.medic.mie-u.ac.jp</mods:affiliation><country 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Medicine, 514-8507, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie</wicri:regionArea></affiliation></author><author><name sortKey="Inoue, Hisae" sort="Inoue, Hisae" uniqKey="Inoue H" first="Hisae" last="Inoue">Hisae Inoue</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, Suzuka University of Medical Science, 510-0293, Suzuka, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Suzuka University of Medical Science, 510-0293, Suzuka, Mie</wicri:regionArea></affiliation></author><author><name sortKey="Yamabayashi, Chihiro" sort="Yamabayashi, Chihiro" uniqKey="Yamabayashi C" first="Chihiro" last="Yamabayashi">Chihiro Yamabayashi</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, Suzuka University of Medical Science, 510-0293, Suzuka, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Suzuka University of Medical Science, 510-0293, Suzuka, Mie</wicri:regionArea></affiliation></author><author><name sortKey="Ito, Morihiro" sort="Ito, Morihiro" uniqKey="Ito M" first="Morihiro" last="Ito">Morihiro Ito</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie</wicri:regionArea></affiliation></author><author><name sortKey="Kawano, Mitsuo" sort="Kawano, Mitsuo" uniqKey="Kawano M" first="Mitsuo" last="Kawano">Mitsuo Kawano</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie</wicri:regionArea></affiliation></author><author><name sortKey="Nishio, Machiko" sort="Nishio, Machiko" uniqKey="Nishio M" first="Machiko" last="Nishio">Machiko Nishio</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie</wicri:regionArea></affiliation></author><author><name sortKey="Tsurudome, Masato" sort="Tsurudome, Masato" uniqKey="Tsurudome M" first="Masato" last="Tsurudome">Masato Tsurudome</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie</wicri:regionArea></affiliation></author><author><name sortKey="Kozuka, Yuji" sort="Kozuka, Yuji" uniqKey="Kozuka Y" first="Yuji" last="Kozuka">Yuji Kozuka</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie</wicri:regionArea></affiliation></author><author><name sortKey="Noda, Naoya" sort="Noda, Naoya" uniqKey="Noda N" first="Naoya" last="Noda">Naoya Noda</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie</wicri:regionArea></affiliation></author><author><name sortKey="Namba, Kazuyoshi" sort="Namba, Kazuyoshi" uniqKey="Namba K" first="Kazuyoshi" last="Namba">Kazuyoshi Namba</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie</wicri:regionArea></affiliation></author><author><name sortKey="O Brien, Myles" sort="O Brien, Myles" uniqKey="O Brien M" first="Myles" last="O'Brien">Myles O'Brien</name><affiliation wicri:level="1"><mods:affiliation>Mie Prefectural College of Nursing, 514-0116, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Mie Prefectural College of Nursing, 514-0116, Tsu, Mie</wicri:regionArea></affiliation></author><author><name sortKey="Ito, Yasuhiko" sort="Ito, Yasuhiko" uniqKey="Ito Y" first="Yasuhiko" last="Ito">Yasuhiko Ito</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Microbiology, Mie University School of Medicine, 514-8507, Tsu, Mie</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: Ito@doc.medic.mie-u.ac.jp</mods:affiliation><country wicri:rule="url">Japon</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Medical Microbiology and Immunology</title><title level="j" type="abbrev">Med Microbiol Immunol</title><idno type="ISSN">0300-8584</idno><idno type="eISSN">1432-1831</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2000-06-01">2000-06-01</date><biblScope unit="volume">188</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="185">185</biblScope><biblScope unit="page" to="189">189</biblScope></imprint><idno type="ISSN">0300-8584</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0300-8584</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Incomplete replication</term><term>Key words Human parainfluenza virus type 4</term><term>L929 cell line</term><term>LLC-MK2 cell line</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Human parainfluenza virus type 4A (hPIV-4A) and type 4B (hPIV-4B) were tested for their ability to replicate in the monkey kidney LLC-MK2 cell line (MK2 cells) and the murine L929 cell line (L929 cells). These cells are normally non-permissive for replication of hPIV-4; however, treatment with acetylated trypsin led to virus replication in MK2 cells, but was less effective for L929 cells. Endogenously produced interferon (IFN) played no role in virus replication in L929 cells. Synthesis of virus-specific polypeptides was suppressed in L929 cells. WhereasNP-mRNA and HN-mRNA were detected in MK2 cells, no HN-mRNA was detected in L929 cells. These results indicate that hPIV-4 can infect both MK2 cells and L929 cells. In MK2 cells, when protease exists in the extracellular medium, hPIV-4 exhibits multistep growth. In L929 cells, however, the cause of incomplete replication might be lack of other unknown factors.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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