Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes
Identifieur interne : 000345 ( Istex/Curation ); précédent : 000344; suivant : 000346Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes
Auteurs : Kimberly H. Abramo [États-Unis] ; J. Bruce Pitner [États-Unis] ; Linda B. Mcgown [États-Unis]Source :
- Biospectroscopy [ 1075-4261 ] ; 1998.
English descriptors
- Teeft :
- Absorbance, Absorption spectra, Anisotropy, Anisotropy measurements, Base composition, Binding constants, Binding interactions, Binding modes, Binding selectivity, Binding stoichiometries, Binding stoichiometry, Biosas, Circular dichroism, Circular dichroism spectra, Conformational differences, Dichroism, Duke university, Dye, Emission beam, Emission wavelength, Environmental health sciences, Equilibrium binding constants, Equilibrium constants, Excitonic, Fluorescence anisotropy, Greater degree, Homogeneous base composition, Human virus type, Indicator dyes, Individual ligand, Intramolecular, Intramolecular excitonic, John wiley sons, Lifetime distributions, Ligand, Ligand concentrations, Mcgown, Nals, National institute, National institutes, Other hand, Other ligands, Oxazole, Photophysical properties, Pitner, Quantum yields, Santa clara, Single strands, Spec, Spectronics instruments, Spectroscopic studies, Stoichiometry, Target molecules, Toxicology training grant program fellowship, Uorescence, Uorescent dyes, Yoyo, Yoyo peak.
Abstract
Interactions between short single‐stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G‐quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO‐pro‐1 iodide (YO) and YOYO‐1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G‐quartet forming ligands, a 20‐mer of sequence 5′‐GGTTTT‐GGTTTTGGTTTTGG‐3′, shows distinctly different interactions from the other three ligands, all of which are 15‐mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single‐stranded DNA. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 27–35, 1998
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DOI: 10.1002/(SICI)1520-6343(1998)4:1<27::AID-BSPY3>3.0.CO;2-P
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Gross Chemical Laboratory, Duke University, Durham</wicri:cityArea></affiliation><affiliation wicri:level="2"><mods:affiliation>Correspondence address: Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Correspondence address: Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biospectroscopy</title><title level="j" type="alt">BIOSPECTROSCOPY</title><idno type="ISSN">1075-4261</idno><idno type="eISSN">1520-6343</idno><imprint><biblScope unit="vol">4</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="27">27</biblScope><biblScope unit="page" to="35">35</biblScope><biblScope unit="page-count">9</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1998">1998</date></imprint><idno type="ISSN">1075-4261</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1075-4261</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Absorbance</term><term>Absorption spectra</term><term>Anisotropy</term><term>Anisotropy measurements</term><term>Base composition</term><term>Binding constants</term><term>Binding interactions</term><term>Binding modes</term><term>Binding selectivity</term><term>Binding stoichiometries</term><term>Binding stoichiometry</term><term>Biosas</term><term>Circular dichroism</term><term>Circular dichroism spectra</term><term>Conformational differences</term><term>Dichroism</term><term>Duke university</term><term>Dye</term><term>Emission beam</term><term>Emission wavelength</term><term>Environmental health sciences</term><term>Equilibrium binding constants</term><term>Equilibrium constants</term><term>Excitonic</term><term>Fluorescence anisotropy</term><term>Greater degree</term><term>Homogeneous base composition</term><term>Human virus type</term><term>Indicator dyes</term><term>Individual ligand</term><term>Intramolecular</term><term>Intramolecular excitonic</term><term>John wiley sons</term><term>Lifetime distributions</term><term>Ligand</term><term>Ligand concentrations</term><term>Mcgown</term><term>Nals</term><term>National institute</term><term>National institutes</term><term>Other hand</term><term>Other ligands</term><term>Oxazole</term><term>Photophysical properties</term><term>Pitner</term><term>Quantum yields</term><term>Santa clara</term><term>Single strands</term><term>Spec</term><term>Spectronics instruments</term><term>Spectroscopic studies</term><term>Stoichiometry</term><term>Target molecules</term><term>Toxicology training grant program fellowship</term><term>Uorescence</term><term>Uorescent dyes</term><term>Yoyo</term><term>Yoyo peak</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Interactions between short single‐stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G‐quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO‐pro‐1 iodide (YO) and YOYO‐1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G‐quartet forming ligands, a 20‐mer of sequence 5′‐GGTTTT‐GGTTTTGGTTTTGG‐3′, shows distinctly different interactions from the other three ligands, all of which are 15‐mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single‐stranded DNA. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 27–35, 1998</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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