Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes
Identifieur interne : 000345 ( Istex/Corpus ); précédent : 000344; suivant : 000346Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes
Auteurs : Kimberly H. Abramo ; J. Bruce Pitner ; Linda B. McgownSource :
- Biospectroscopy [ 1075-4261 ] ; 1998.
English descriptors
- Teeft :
- Absorbance, Absorption spectra, Anisotropy, Anisotropy measurements, Base composition, Binding constants, Binding interactions, Binding modes, Binding selectivity, Binding stoichiometries, Binding stoichiometry, Biosas, Circular dichroism, Circular dichroism spectra, Conformational differences, Dichroism, Duke university, Dye, Emission beam, Emission wavelength, Environmental health sciences, Equilibrium binding constants, Equilibrium constants, Excitonic, Fluorescence anisotropy, Greater degree, Homogeneous base composition, Human virus type, Indicator dyes, Individual ligand, Intramolecular, Intramolecular excitonic, John wiley sons, Lifetime distributions, Ligand, Ligand concentrations, Mcgown, Nals, National institute, National institutes, Other hand, Other ligands, Oxazole, Photophysical properties, Pitner, Quantum yields, Santa clara, Single strands, Spec, Spectronics instruments, Spectroscopic studies, Stoichiometry, Target molecules, Toxicology training grant program fellowship, Uorescence, Uorescent dyes, Yoyo, Yoyo peak.
Abstract
Interactions between short single‐stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G‐quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO‐pro‐1 iodide (YO) and YOYO‐1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G‐quartet forming ligands, a 20‐mer of sequence 5′‐GGTTTT‐GGTTTTGGTTTTGG‐3′, shows distinctly different interactions from the other three ligands, all of which are 15‐mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single‐stranded DNA. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 27–35, 1998
Url:
DOI: 10.1002/(SICI)1520-6343(1998)4:1<27::AID-BSPY3>3.0.CO;2-P
Links to Exploration step
ISTEX:96678992D4CBAF5486AF7444019CB99E3E2BA55CLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes</title><author><name sortKey="Abramo, Kimberly H" sort="Abramo, Kimberly H" uniqKey="Abramo K" first="Kimberly H." last="Abramo">Kimberly H. Abramo</name><affiliation><mods:affiliation>Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</mods:affiliation></affiliation></author><author><name sortKey="Pitner, J Bruce" sort="Pitner, J Bruce" uniqKey="Pitner J" first="J. Bruce" last="Pitner">J. Bruce Pitner</name><affiliation><mods:affiliation>Becton Dickinson Research Center, Research Triangle Park, North Carolina 27709</mods:affiliation></affiliation></author><author><name sortKey="Mcgown, Linda B" sort="Mcgown, Linda B" uniqKey="Mcgown L" first="Linda B." last="Mcgown">Linda B. Mcgown</name><affiliation><mods:affiliation>Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:96678992D4CBAF5486AF7444019CB99E3E2BA55C</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1002/(SICI)1520-6343(1998)4:1<27::AID-BSPY3>3.0.CO;2-P</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-8B6PJ3X5-V/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000345</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000345</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes</title><author><name sortKey="Abramo, Kimberly H" sort="Abramo, Kimberly H" uniqKey="Abramo K" first="Kimberly H." last="Abramo">Kimberly H. Abramo</name><affiliation><mods:affiliation>Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</mods:affiliation></affiliation></author><author><name sortKey="Pitner, J Bruce" sort="Pitner, J Bruce" uniqKey="Pitner J" first="J. Bruce" last="Pitner">J. Bruce Pitner</name><affiliation><mods:affiliation>Becton Dickinson Research Center, Research Triangle Park, North Carolina 27709</mods:affiliation></affiliation></author><author><name sortKey="Mcgown, Linda B" sort="Mcgown, Linda B" uniqKey="Mcgown L" first="Linda B." last="Mcgown">Linda B. Mcgown</name><affiliation><mods:affiliation>Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biospectroscopy</title><title level="j" type="alt">BIOSPECTROSCOPY</title><idno type="ISSN">1075-4261</idno><idno type="eISSN">1520-6343</idno><imprint><biblScope unit="vol">4</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="27">27</biblScope><biblScope unit="page" to="35">35</biblScope><biblScope unit="page-count">9</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1998">1998</date></imprint><idno type="ISSN">1075-4261</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1075-4261</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Absorbance</term><term>Absorption spectra</term><term>Anisotropy</term><term>Anisotropy measurements</term><term>Base composition</term><term>Binding constants</term><term>Binding interactions</term><term>Binding modes</term><term>Binding selectivity</term><term>Binding stoichiometries</term><term>Binding stoichiometry</term><term>Biosas</term><term>Circular dichroism</term><term>Circular dichroism spectra</term><term>Conformational differences</term><term>Dichroism</term><term>Duke university</term><term>Dye</term><term>Emission beam</term><term>Emission wavelength</term><term>Environmental health sciences</term><term>Equilibrium binding constants</term><term>Equilibrium constants</term><term>Excitonic</term><term>Fluorescence anisotropy</term><term>Greater degree</term><term>Homogeneous base composition</term><term>Human virus type</term><term>Indicator dyes</term><term>Individual ligand</term><term>Intramolecular</term><term>Intramolecular excitonic</term><term>John wiley sons</term><term>Lifetime distributions</term><term>Ligand</term><term>Ligand concentrations</term><term>Mcgown</term><term>Nals</term><term>National institute</term><term>National institutes</term><term>Other hand</term><term>Other ligands</term><term>Oxazole</term><term>Photophysical properties</term><term>Pitner</term><term>Quantum yields</term><term>Santa clara</term><term>Single strands</term><term>Spec</term><term>Spectronics instruments</term><term>Spectroscopic studies</term><term>Stoichiometry</term><term>Target molecules</term><term>Toxicology training grant program fellowship</term><term>Uorescence</term><term>Uorescent dyes</term><term>Yoyo</term><term>Yoyo peak</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Interactions between short single‐stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G‐quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO‐pro‐1 iodide (YO) and YOYO‐1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G‐quartet forming ligands, a 20‐mer of sequence 5′‐GGTTTT‐GGTTTTGGTTTTGG‐3′, shows distinctly different interactions from the other three ligands, all of which are 15‐mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single‐stranded DNA. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 27–35, 1998</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>yoyo</json:string><json:string>ligand</json:string><json:string>dye</json:string><json:string>stoichiometry</json:string><json:string>uorescence</json:string><json:string>excitonic</json:string><json:string>biosas</json:string><json:string>oxazole</json:string><json:string>mcgown</json:string><json:string>intramolecular</json:string><json:string>anisotropy</json:string><json:string>absorbance</json:string><json:string>dichroism</json:string><json:string>pitner</json:string><json:string>nals</json:string><json:string>binding interactions</json:string><json:string>other ligands</json:string><json:string>spec</json:string><json:string>photophysical properties</json:string><json:string>indicator dyes</json:string><json:string>conformational differences</json:string><json:string>lifetime distributions</json:string><json:string>target molecules</json:string><json:string>equilibrium binding constants</json:string><json:string>absorption spectra</json:string><json:string>duke university</json:string><json:string>fluorescence anisotropy</json:string><json:string>spectroscopic studies</json:string><json:string>circular dichroism spectra</json:string><json:string>john wiley sons</json:string><json:string>human virus type</json:string><json:string>national institute</json:string><json:string>base composition</json:string><json:string>national institutes</json:string><json:string>anisotropy measurements</json:string><json:string>spectronics instruments</json:string><json:string>santa clara</json:string><json:string>ligand concentrations</json:string><json:string>emission beam</json:string><json:string>emission wavelength</json:string><json:string>toxicology training grant program fellowship</json:string><json:string>single strands</json:string><json:string>intramolecular excitonic</json:string><json:string>environmental health sciences</json:string><json:string>yoyo peak</json:string><json:string>binding stoichiometries</json:string><json:string>other hand</json:string><json:string>binding modes</json:string><json:string>circular dichroism</json:string><json:string>quantum yields</json:string><json:string>binding selectivity</json:string><json:string>binding stoichiometry</json:string><json:string>equilibrium constants</json:string><json:string>individual ligand</json:string><json:string>binding constants</json:string><json:string>greater degree</json:string><json:string>homogeneous base composition</json:string><json:string>uorescent dyes</json:string></teeft></keywords><author><json:item><name>Kimberly H. Abramo</name><affiliations><json:string>Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</json:string></affiliations></json:item><json:item><name>J. Bruce Pitner</name><affiliations><json:string>Becton Dickinson Research Center, Research Triangle Park, North Carolina 27709</json:string></affiliations></json:item><json:item><name>Linda B. McGown</name><affiliations><json:string>Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</json:string><json:string>Correspondence address: Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>DNA dyes</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>single‐stranded DNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>oxazole yellow</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>fluorescent dyes</value></json:item></subject><articleId><json:string>BSPY3</json:string></articleId><arkIstex>ark:/67375/WNG-8B6PJ3X5-V</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Interactions between short single‐stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G‐quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO‐pro‐1 iodide (YO) and YOYO‐1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G‐quartet forming ligands, a 20‐mer of sequence 5′‐GGTTTT‐GGTTTTGGTTTTGG‐3′, shows distinctly different interactions from the other three ligands, all of which are 15‐mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single‐stranded DNA. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 27–35, 1998</abstract><qualityIndicators><score>7.747</score><pdfWordCount>3899</pdfWordCount><pdfCharCount>23693</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><pdfWordsPerPage>433</pdfWordsPerPage><pdfText>true</pdfText><refBibsNative>true</refBibsNative><abstractWordCount>154</abstractWordCount><abstractCharCount>1135</abstractCharCount><keywordCount>4</keywordCount></qualityIndicators><title>Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes</title><genre><json:string>article</json:string></genre><host><title>Biospectroscopy</title><language><json:string>unknown</json:string></language><doi><json:string>10.1002/(ISSN)1520-6343</json:string></doi><issn><json:string>1075-4261</json:string></issn><eissn><json:string>1520-6343</json:string></eissn><publisherId><json:string>BSPY</json:string></publisherId><volume>4</volume><issue>1</issue><pages><first>27</first><last>35</last><total>9</total></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1998</json:string><json:string>01-09-98</json:string></date><geogName></geogName><orgName><json:string>Sons, Inc.</json:string><json:string>Becton Dickinson Research Center, Research Triangle Park, North Carolina</json:string><json:string>Spectronics Instruments</json:string><json:string>National Institutes of Health, National Institute of Environmental Health Sciences</json:string><json:string>Gross Chemical Laboratory, Duke University, Durham, North Carolina</json:string><json:string>Spectronics Instruments, Rochester</json:string><json:string>Jasco Corp., Tokyo, Japan</json:string><json:string>Duke University</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>C. Dye</json:string><json:string>John Wiley</json:string><json:string>C. Excitation</json:string><json:string>L. B. McGown</json:string></persName><placeName><json:string>Santa Clara</json:string><json:string>CA</json:string><json:string>MN</json:string></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-8B6PJ3X5-V</json:string></ark><categories><wos></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - biophysics</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - General Biochemistry, Genetics and Molecular Biology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><doi><json:string>10.1002/(SICI)1520-6343(1998)4:1>27::AID-BSPY3>3.0.CO;2-P</json:string></doi><id>96678992D4CBAF5486AF7444019CB99E3E2BA55C</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-8B6PJ3X5-V/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-8B6PJ3X5-V/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/WNG-8B6PJ3X5-V/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main">Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes</title><title level="a" type="short" xml:lang="en">Single‐stranded DNA ligands and oxazole yellow dyes</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><availability><licence>Copyright © 1998 John Wiley & Sons, Inc.</licence></availability><date type="published" when="1998"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main">Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes</title><title level="a" type="short" xml:lang="en">Single‐stranded DNA ligands and oxazole yellow dyes</title><author xml:id="author-0000"><persName><forename type="first">Kimberly H.</forename><surname>Abramo</surname></persName><affiliation><orgName type="division">Department of Chemistry</orgName><address><addrLine>Box 90346</addrLine><addrLine>P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation></author><author xml:id="author-0001"><persName><forename type="first">J. Bruce</forename><surname>Pitner</surname></persName><affiliation><orgName type="institution">Becton Dickinson Research Center</orgName><orgName type="laboratory">Research Triangle Park</orgName><address><addrLine>North Carolina 27709</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation></author><author xml:id="author-0002" role="corresp"><persName><forename type="first">Linda B.</forename><surname>McGown</surname></persName><affiliation><orgName type="division">Department of Chemistry</orgName><address><addrLine>Box 90346</addrLine><addrLine>P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation></author><idno type="istex">96678992D4CBAF5486AF7444019CB99E3E2BA55C</idno><idno type="ark">ark:/67375/WNG-8B6PJ3X5-V</idno><idno type="DOI">10.1002/(SICI)1520-6343(1998)4:1<27::AID-BSPY3>3.0.CO;2-P</idno><idno type="unit">BSPY3</idno><idno type="toTypesetVersion">file:BSPY.BSPY3.pdf</idno></analytic><monogr><title level="j" type="main">Biospectroscopy</title><title level="j" type="alt">BIOSPECTROSCOPY</title><idno type="pISSN">1075-4261</idno><idno type="eISSN">1520-6343</idno><idno type="book-DOI">10.1002/(ISSN)1520-6343</idno><idno type="book-part-DOI">10.1002/(SICI)1520-6343(1998)4:1<>1.0.CO;2-B</idno><idno type="product">BSPY</idno><imprint><biblScope unit="vol">4</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="27">27</biblScope><biblScope unit="page" to="35">35</biblScope><biblScope unit="page-count">9</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1998"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><encodingDesc><schemaRef type="ODD" url="https://xml-schema.delivery.istex.fr/tei-istex.odd"></schemaRef><appInfo><application ident="pub2tei" version="1.0.10" when="2019-12-20"><label>pub2TEI-ISTEX</label><desc>A set of style sheets for converting XML documents encoded in various scientific publisher formats into a common TEI format.
<ref target="http://www.tei-c.org/">We use TEI</ref></desc></application></appInfo></encodingDesc><profileDesc><abstract xml:lang="en" style="main"><head>Abstract</head><p>Interactions between short single‐stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G‐quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO‐pro‐1 iodide (YO) and YOYO‐1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G‐quartet forming ligands, a 20‐mer of sequence 5′‐GGTTTT‐GGTTTTGGTTTTGG‐3′, shows distinctly different interactions from the other three ligands, all of which are 15‐mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single‐stranded DNA. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 27–35, 1998</p></abstract><textClass><keywords xml:lang="en"><term xml:id="kwd1">DNA dyes</term><term xml:id="kwd2">single‐stranded DNA</term><term xml:id="kwd3">oxazole yellow</term><term xml:id="kwd4">fluorescent dyes</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2019-12-20" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-8B6PJ3X5-V/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1520-6343</doi><issn type="print">1075-4261</issn><issn type="electronic">1520-6343</issn><idGroup><id type="product" value="BSPY"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="BIOSPECTROSCOPY">Biospectroscopy</title><title type="short">Biospectroscopy</title></titleGroup></publicationMeta><publicationMeta level="part" position="10"><doi origin="wiley" registered="yes">10.1002/(SICI)1520-6343(1998)4:1<>1.0.CO;2-B</doi><numberingGroup><numbering type="journalVolume" number="4">4</numbering><numbering type="journalIssue">1</numbering></numberingGroup><coverDate startDate="1998">1998</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="3" status="forIssue"><doi origin="wiley" registered="yes">10.1002/(SICI)1520-6343(1998)4:1<27::AID-BSPY3>3.0.CO;2-P</doi><idGroup><id type="unit" value="BSPY3"></id></idGroup><countGroup><count type="pageTotal" number="9"></count></countGroup><copyright ownership="publisher">Copyright © 1998 John Wiley & Sons, Inc.</copyright><eventGroup><event type="manuscriptReceived" date="1996-12-06"></event><event type="manuscriptRevised" date="1997-03-26"></event><event type="manuscriptAccepted" date="1997-06-06"></event><event type="firstOnline" date="1998-12-07"></event><event type="publishedOnlineFinalForm" date="1998-12-07"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-04"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-08"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst">27</numbering><numbering type="pageLast">35</numbering></numberingGroup><correspondenceTo>Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:BSPY.BSPY3.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="7"></count><count type="tableTotal" number="3"></count><count type="referenceTotal" number="17"></count><count type="wordTotal" number="3791"></count></countGroup><titleGroup><title type="main" xml:lang="en">Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes</title><title type="short" xml:lang="en">Single‐stranded DNA ligands and oxazole yellow dyes</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Kimberly H.</givenNames><familyName>Abramo</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af2"><personName><givenNames>J. Bruce</givenNames><familyName>Pitner</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Linda B.</givenNames><familyName>McGown</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="US" type="organization"><unparsedAffiliation>Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="US" type="organization"><unparsedAffiliation>Becton Dickinson Research Center, Research Triangle Park, North Carolina 27709</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">DNA dyes</keyword><keyword xml:id="kwd2">single‐stranded DNA</keyword><keyword xml:id="kwd3">oxazole yellow</keyword><keyword xml:id="kwd4">fluorescent dyes</keyword></keywordGroup><fundingInfo><fundingAgency>National Institutes of Health</fundingAgency><fundingNumber>T32ES07031‐17</fundingNumber></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Interactions between short single‐stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G‐quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO‐pro‐1 iodide (YO) and YOYO‐1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G‐quartet forming ligands, a 20‐mer of sequence 5′‐GGTTTT‐GGTTTTGGTTTTGG‐3′, shows distinctly different interactions from the other three ligands, all of which are 15‐mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single‐stranded DNA. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 27–35, 1998</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Single‐stranded DNA ligands and oxazole yellow dyes</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes</title></titleInfo><name type="personal"><namePart type="given">Kimberly H.</namePart><namePart type="family">Abramo</namePart><affiliation>Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. Bruce</namePart><namePart type="family">Pitner</namePart><affiliation>Becton Dickinson Research Center, Research Triangle Park, North Carolina 27709</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Linda B.</namePart><namePart type="family">McGown</namePart><affiliation>Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</affiliation><affiliation>Correspondence address: Department of Chemistry, Box 90346, P.M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708‐0346</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">1998</dateIssued><dateCaptured encoding="w3cdtf">1996-12-06</dateCaptured><dateValid encoding="w3cdtf">1997-06-06</dateValid><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">7</extent><extent unit="tables">3</extent><extent unit="references">17</extent><extent unit="words">3791</extent></physicalDescription><abstract lang="en">Interactions between short single‐stranded DNA ligands and fluorescent DNA indicator dyes were used to investigate binding selectivity of the ligands. Conformational differences among four DNA ligands of different sequence and structure, including two that form a G‐quartet and two that do not, were confirmed by circular dichroism spectroscopy. Their interactions with indicator dyes YO‐pro‐1 iodide (YO) and YOYO‐1 iodide (YOYO) were probed using measurements of dye absorbance; induced circular dichroism; and fluorescence spectra, anisotropy, and lifetime. Equilibrium binding constants and stoichiometry were determined as well. Results indicate significant differences among the dye interactions and binding stoichiometries of the four ligands. One of the G‐quartet forming ligands, a 20‐mer of sequence 5′‐GGTTTT‐GGTTTTGGTTTTGG‐3′, shows distinctly different interactions from the other three ligands, all of which are 15‐mers. These studies illustrate the importance of sequence and conformation in determining the binding interactions of short single‐stranded DNA. © 1998 John Wiley & Sons, Inc. Biospectroscopy 4: 27–35, 1998</abstract><note type="funding">National Institutes of Health - No. T32ES07031‐17; </note><subject lang="en"><genre>keywords</genre><topic>DNA dyes</topic><topic>single‐stranded DNA</topic><topic>oxazole yellow</topic><topic>fluorescent dyes</topic></subject><relatedItem type="host"><titleInfo><title>Biospectroscopy</title></titleInfo><titleInfo type="abbreviated"><title>Biospectroscopy</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">1075-4261</identifier><identifier type="eISSN">1520-6343</identifier><identifier type="DOI">10.1002/(ISSN)1520-6343</identifier><identifier type="PublisherID">BSPY</identifier><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>4</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>27</start><end>35</end><total>9</total></extent></part></relatedItem><relatedItem type="references" displayLabel="cit1"><titleInfo><title>Evolution in a test tube</title></titleInfo><name type="personal"><namePart type="given">K. F.</namePart><namePart type="family">Schmidt</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">K. F. Schmidt, “Evolution in a test tube,” Science News, 144, 90 (1993).</note><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>144</number></detail><extent unit="pages"><start>90</start></extent></part><relatedItem type="host"><titleInfo><title>Science News</title></titleInfo><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>144</number></detail><extent unit="pages"><start>90</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit2"><titleInfo><title>The nucleic acid ligand: A new tool for molecular recognition</title></titleInfo><name type="personal"><namePart type="given">L. B.</namePart><namePart type="family">McGown</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. J.</namePart><namePart type="family">Joseph</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. B.</namePart><namePart type="family">Pitner</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G. P.</namePart><namePart type="family">Vonk</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. P.</namePart><namePart type="family">Linn</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">L. B. McGown, M. J. Joseph, J. B. Pitner, G. P. Vonk, and C. P. Linn, “The nucleic acid ligand: A new tool for molecular recognition,” Anal. Chem., 67, 663A (1995).</note><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>67</number></detail><extent unit="pages"><start>663A</start></extent></part><relatedItem type="host"><titleInfo><title>Anal. Chem.</title></titleInfo><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>67</number></detail><extent unit="pages"><start>663A</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit3"><titleInfo><title>RNA pseudoknots that inhibit human immunodeficiency virus type 1 reverse transcriptase</title></titleInfo><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Tuerk</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">MacDougal</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Gold</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">C. Tuerk, S. MacDougal, and L. Gold, “RNA pseudoknots that inhibit human immunodeficiency virus type 1 reverse transcriptase,” Proc. Natl. Acad. Sci. USA, 89, 6988 (1992).</note><part><date>1992</date><detail type="volume"><caption>vol.</caption><number>89</number></detail><extent unit="pages"><start>6988</start></extent></part><relatedItem type="host"><titleInfo><title>Proc. Natl. Acad. Sci. USA</title></titleInfo><part><date>1992</date><detail type="volume"><caption>vol.</caption><number>89</number></detail><extent unit="pages"><start>6988</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit4"><titleInfo><title>An RNA motif that binds ATP</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Sassanfar</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. W.</namePart><namePart type="family">Szostak</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">M. Sassanfar and J. W. Szostak, “An RNA motif that binds ATP,” Science, 364, 550 (1993).</note><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>364</number></detail><extent unit="pages"><start>550</start></extent></part><relatedItem type="host"><titleInfo><title>Science</title></titleInfo><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>364</number></detail><extent unit="pages"><start>550</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit5"><titleInfo><title>Selection of singlestranded DNA molecules that bind and inhibit human thrombin</title></titleInfo><name type="personal"><namePart type="given">L. C.</namePart><namePart type="family">Bock</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L. C.</namePart><namePart type="family">Griffin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. A.</namePart><namePart type="family">Lantham</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E. H.</namePart><namePart type="family">Vermaas</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. J.</namePart><namePart type="family">Toole</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">L. C. Bock, L. C. Griffin, J. A. Lantham, E. H. Vermaas, and J. J. Toole, “Selection of singlestranded DNA molecules that bind and inhibit human thrombin,” Nature, 355, 564 (1992).</note><part><date>1992</date><detail type="volume"><caption>vol.</caption><number>355</number></detail><extent unit="pages"><start>564</start></extent></part><relatedItem type="host"><titleInfo><title>Nature</title></titleInfo><part><date>1992</date><detail type="volume"><caption>vol.</caption><number>355</number></detail><extent unit="pages"><start>564</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit6"><titleInfo><title>Reconciliation of the X‐ray and NMR structures of the thrombin‐binding aptamer d(GGTTGGTGTGGTTGG)</title></titleInfo><name type="personal"><namePart type="given">J. A.</namePart><namePart type="family">Kelly</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Feigon</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T. O.</namePart><namePart type="family">Yeates</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">J. A. Kelly, J. Feigon, and T. O. Yeates, “Reconciliation of the X‐ray and NMR structures of the thrombin‐binding aptamer d(GGTTGGTGTGGTTGG),” J. Mol. Biol., 256, 417 (1996).</note><part><date>1996</date><detail type="volume"><caption>vol.</caption><number>256</number></detail><extent unit="pages"><start>417</start></extent></part><relatedItem type="host"><titleInfo><title>J. Mol. Biol.</title></titleInfo><part><date>1996</date><detail type="volume"><caption>vol.</caption><number>256</number></detail><extent unit="pages"><start>417</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit7"><titleInfo><title>A DNA aptamer which binds to and inhibits thrombin exhibits a new structural in solution</title></titleInfo><name type="personal"><namePart type="given">K. Y.</namePart><namePart type="family">Wang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">McCurdy</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. G.</namePart><namePart type="family">Shea</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Swaminathan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P. H.</namePart><namePart type="family">Bolton</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">K. Y. Wang, S. McCurdy, R. G. Shea, S. Swaminathan, and P. H. Bolton, “A DNA aptamer which binds to and inhibits thrombin exhibits a new structural in solution,” Biochemistry, 32, 1899 (1993).</note><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>32</number></detail><extent unit="pages"><start>1899</start></extent></part><relatedItem type="host"><titleInfo><title>Biochemistry</title></titleInfo><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>32</number></detail><extent unit="pages"><start>1899</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit8"><titleInfo><title>Thrombin‐binding DNA aptamer forms a unimolecular quadruplex structure in solution</title></titleInfo><name type="personal"><namePart type="given">R. F.</namePart><namePart type="family">Macaya</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Schultze</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F. W.</namePart><namePart type="family">Smith</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. A.</namePart><namePart type="family">Roe</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Feigon</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">R. F. Macaya, P. Schultze, F. W. Smith, J. A. Roe, and J. Feigon, “Thrombin‐binding DNA aptamer forms a unimolecular quadruplex structure in solution,” Proc. Natl. Acad. Sci. USA, 90, 3745 (1993).</note><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>90</number></detail><extent unit="pages"><start>3745</start></extent></part><relatedItem type="host"><titleInfo><title>Proc. Natl. Acad. Sci. USA</title></titleInfo><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>90</number></detail><extent unit="pages"><start>3745</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit9"><titleInfo><title>Threedimensional solution structure of the thrombin‐binding DNA aptamer d(GGTTGGTGTGGTTGG)</title></titleInfo><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Schultze</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. F.</namePart><namePart type="family">Macaya</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Feigon</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">P. Schultze, R. F. Macaya, and J. Feigon, “Threedimensional solution structure of the thrombin‐binding DNA aptamer d(GGTTGGTGTGGTTGG),” J. Mol. Biol., 235, 1532 (1994).</note><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>235</number></detail><extent unit="pages"><start>1532</start></extent></part><relatedItem type="host"><titleInfo><title>J. Mol. Biol.</title></titleInfo><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>235</number></detail><extent unit="pages"><start>1532</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit10"><titleInfo><title>Spectroscopic studies of YO and YOYO fluorescent dyes in a thrombin‐binding DNA ligand</title></titleInfo><name type="personal"><namePart type="given">M. J.</namePart><namePart type="family">Joseph</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. C.</namePart><namePart type="family">Taylor</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L. B.</namePart><namePart type="family">McGown</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. B.</namePart><namePart type="family">Pitner</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. P.</namePart><namePart type="family">Linn</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">M. J. Joseph, J. C. Taylor, L. B. McGown, J. B. Pitner, and C. P. Linn, “Spectroscopic studies of YO and YOYO fluorescent dyes in a thrombin‐binding DNA ligand,” Biospectroscopy, 2, 173 (1996).</note><part><date>1996</date><detail type="volume"><caption>vol.</caption><number>2</number></detail><extent unit="pages"><start>173</start></extent></part><relatedItem type="host"><titleInfo><title>Biospectroscopy</title></titleInfo><part><date>1996</date><detail type="volume"><caption>vol.</caption><number>2</number></detail><extent unit="pages"><start>173</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit11"><titleInfo><title>Fluorometric assay using dimeric dyes for double‐and single‐stranded DNA and RNA with picogram sensitivity</title></titleInfo><name type="personal"><namePart type="given">H. S.</namePart><namePart type="family">Rye</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. M.</namePart><namePart type="family">Dabora</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. A.</namePart><namePart type="family">Quesada</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. A.</namePart><namePart type="family">Mathies</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A. N.</namePart><namePart type="family">Glazer</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">H. S. Rye, J. M. Dabora, M. A. Quesada, R. A. Mathies, and A. N. Glazer, “Fluorometric assay using dimeric dyes for double‐and single‐stranded DNA and RNA with picogram sensitivity,” Anal. Biochem., 208, 144 (1993).</note><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>208</number></detail><extent unit="pages"><start>144</start></extent></part><relatedItem type="host"><titleInfo><title>Anal. Biochem.</title></titleInfo><part><date>1993</date><detail type="volume"><caption>vol.</caption><number>208</number></detail><extent unit="pages"><start>144</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit12"><titleInfo><title>J. R. Lakowicz,Principles of Fluorescence Spectroscopy,Plenum Press, New York,1983.</title></titleInfo><note type="citation/reference">J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Plenum Press, New York, 1983.</note><name type="personal"><namePart type="given">J. R.</namePart><namePart type="family">Lakowicz</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>book</genre><originInfo><publisher>Plenum Press</publisher><place><placeTerm type="text">New York</placeTerm></place></originInfo><part><date>1983</date></part></relatedItem><relatedItem type="references" displayLabel="cit13"><titleInfo><title>Optical and photophysical properties of the oxazole yellow DNA probes YO and YOYO</title></titleInfo><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Carlsson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Larsson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Jonsson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Albinsson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Norden</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">C. Carlsson, A. Larsson, M. Jonsson, B. Albinsson, and B. Norden, “Optical and photophysical properties of the oxazole yellow DNA probes YO and YOYO,” J. Phys. Chem., 98, 10313 (1994).</note><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>98</number></detail><extent unit="pages"><start>10313</start></extent></part><relatedItem type="host"><titleInfo><title>J. Phys. Chem.</title></titleInfo><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>98</number></detail><extent unit="pages"><start>10313</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit14"><titleInfo><title>Oxazole yellow dye interactions with short DNA oligomers of homogeneous base composition and their hybrids</title></titleInfo><name type="personal"><namePart type="given">L. D.</namePart><namePart type="family">Simon</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K. H.</namePart><namePart type="family">Abramo</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. K.</namePart><namePart type="family">Sell</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L. B.</namePart><namePart type="family">McGown</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">L. D. Simon, K. H. Abramo, J. K. Sell, and L. B. McGown, “Oxazole yellow dye interactions with short DNA oligomers of homogeneous base composition and their hybrids,” Biospectroscopy 4, 17–25 (1998).</note><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>4</number></detail><extent unit="pages"><start>17</start><end>25</end></extent></part><relatedItem type="host"><titleInfo><title>Biospectroscopy</title></titleInfo><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>4</number></detail><extent unit="pages"><start>17</start><end>25</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit15"><titleInfo><title>Chemical probe for glycosidic conformation in telomeric DNAs</title></titleInfo><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Dias</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. L.</namePart><namePart type="family">Battiste</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. R.</namePart><namePart type="family">Williamson</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">E. Dias, J. L. Battiste, and J. R. Williamson, “Chemical probe for glycosidic conformation in telomeric DNAs,” J. Am. Chem. Soc., 116, 4479 (1994).</note><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>116</number></detail><extent unit="pages"><start>4479</start></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Chem. Soc.</title></titleInfo><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>116</number></detail><extent unit="pages"><start>4479</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit16"><titleInfo><title>Characterization of the binding of the fluorescent dyes YO and YOYO to DNA by polarized light spectroscopy</title></titleInfo><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Larsson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Carlsson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Jonsson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B. J.</namePart><namePart type="family">Albinsson</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">A. Larsson, C. Carlsson, M. Jonsson, and B. J. Albinsson, “Characterization of the binding of the fluorescent dyes YO and YOYO to DNA by polarized light spectroscopy,” J. Am. Chem. Soc., 116, 8459 (1994).</note><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>116</number></detail><extent unit="pages"><start>8459</start></extent></part><relatedItem type="host"><titleInfo><title>J. Am. Chem. Soc.</title></titleInfo><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>116</number></detail><extent unit="pages"><start>8459</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit17"><titleInfo><title>Base‐content dependence of emission enhancements, quantum yields, and lifetimes for cyanine dyes bound to double‐stranded DNA: Photophysical properties of monomeric and bichromophoric DNA strains</title></titleInfo><name type="personal"><namePart type="given">T. L.</namePart><namePart type="family">Netzel</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Nafisi</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Zhao</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. R.</namePart><namePart type="family">Lenhard</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Johnson</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">T. L. Netzel, K. Nafisi, M. Zhao, J. R. Lenhard, and I. Johnson, “Base‐content dependence of emission enhancements, quantum yields, and lifetimes for cyanine dyes bound to double‐stranded DNA: Photophysical properties of monomeric and bichromophoric DNA strains,” J. Phys. Chem., 99, 17936 (1995).</note><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>99</number></detail><extent unit="pages"><start>17936</start></extent></part><relatedItem type="host"><titleInfo><title>J. Phys. Chem.</title></titleInfo><part><date>1995</date><detail type="volume"><caption>vol.</caption><number>99</number></detail><extent unit="pages"><start>17936</start></extent></part></relatedItem></relatedItem><identifier type="istex">96678992D4CBAF5486AF7444019CB99E3E2BA55C</identifier><identifier type="ark">ark:/67375/WNG-8B6PJ3X5-V</identifier><identifier type="DOI">10.1002/(SICI)1520-6343(1998)4:1<27::AID-BSPY3>3.0.CO;2-P</identifier><identifier type="ArticleID">BSPY3</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 1998 John Wiley & Sons, Inc.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Converted from (version ) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2019-11-14</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-8B6PJ3X5-V/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000345 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000345 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:96678992D4CBAF5486AF7444019CB99E3E2BA55C
|texte= Spectroscopic studies of single‐stranded DNA ligands and oxazole yellow dyes
}}
| This area was generated with Dilib version V0.6.33. |  |