Identification of B‐cell epitopes on the betanodavirus capsid protein
Identifieur interne : 000344 ( Istex/Curation ); précédent : 000343; suivant : 000345Identification of B‐cell epitopes on the betanodavirus capsid protein
Auteurs : J Z Costa [Royaume-Uni] ; A. Adams [Royaume-Uni] ; J E Bron [Royaume-Uni] ; K D Thompson [Royaume-Uni] ; W G Starkey [Royaume-Uni] ; R H Richards [Royaume-Uni]Source :
- Journal of Fish Diseases [ 0140-7775 ] ; 2007-07.
Abstract
The pepscan procedure was used to identify betanodavirus B‐cell epitopes recognized by neutralizing mouse monoclonal antibodies (MAbs) and serum samples obtained from sea bass, Dicentrarchus labrax, naturally infected with betanodavirus. Pepscan was performed with a panel of thirty‐four 12‐mer synthetic peptides that mimicked the entire betanodavirus capsid protein. Sea bass serum samples reacted strongly with three regions of the capsid protein comprising amino acid residues 1–32, 91–162 and 181–212. The latter region was also recognized by neutralizing MAbs and coincided with a region of high antigenic propensity identified by an antigen prediction algorithm. These data suggest that a region of the betanodavirus capsid protein spanning amino acid residues 181–212 may represent a neutralization domain that could potentially be used to inform the development of nodavirus vaccines and immunodiagnostic reagents.
Url:
DOI: 10.1111/j.1365-2761.2007.00824.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000344
Links to Exploration step
ISTEX:8ADB04A13BE598E6911D369B8BB910790FEE7552Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of B‐cell epitopes on the betanodavirus capsid protein</title>
<author><name sortKey="Costa, J Z" sort="Costa, J Z" uniqKey="Costa J" first="J Z" last="Costa">J Z Costa</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Adams, A" sort="Adams, A" uniqKey="Adams A" first="A" last="Adams">A. Adams</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bron, J E" sort="Bron, J E" uniqKey="Bron J" first="J E" last="Bron">J E Bron</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Thompson, K D" sort="Thompson, K D" uniqKey="Thompson K" first="K D" last="Thompson">K D Thompson</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Starkey, W G" sort="Starkey, W G" uniqKey="Starkey W" first="W G" last="Starkey">W G Starkey</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Richards, R H" sort="Richards, R H" uniqKey="Richards R" first="R H" last="Richards">R H Richards</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:8ADB04A13BE598E6911D369B8BB910790FEE7552</idno>
<date when="2007" year="2007">2007</date>
<idno type="doi">10.1111/j.1365-2761.2007.00824.x</idno>
<idno type="url">https://api.istex.fr/ark:/67375/WNG-RQ3HTM8M-P/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000344</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000344</idno>
<idno type="wicri:Area/Istex/Curation">000344</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Identification of B‐cell epitopes on the betanodavirus capsid protein</title>
<author><name sortKey="Costa, J Z" sort="Costa, J Z" uniqKey="Costa J" first="J Z" last="Costa">J Z Costa</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Adams, A" sort="Adams, A" uniqKey="Adams A" first="A" last="Adams">A. Adams</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bron, J E" sort="Bron, J E" uniqKey="Bron J" first="J E" last="Bron">J E Bron</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Thompson, K D" sort="Thompson, K D" uniqKey="Thompson K" first="K D" last="Thompson">K D Thompson</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Starkey, W G" sort="Starkey, W G" uniqKey="Starkey W" first="W G" last="Starkey">W G Starkey</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Richards, R H" sort="Richards, R H" uniqKey="Richards R" first="R H" last="Richards">R H Richards</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Aquaculture, University of Stirling, Stirling, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Aquaculture, University of Stirling, Stirling</wicri:regionArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Journal of Fish Diseases</title>
<title level="j" type="alt">JOURNAL OF FISH DISEASES</title>
<idno type="ISSN">0140-7775</idno>
<idno type="eISSN">1365-2761</idno>
<imprint><biblScope unit="vol">30</biblScope>
<biblScope unit="issue">7</biblScope>
<biblScope unit="page" from="419">419</biblScope>
<biblScope unit="page" to="426">426</biblScope>
<biblScope unit="page-count">8</biblScope>
<publisher>Blackwell Publishing Ltd</publisher>
<pubPlace>Oxford, UK</pubPlace>
<date type="published" when="2007-07">2007-07</date>
</imprint>
<idno type="ISSN">0140-7775</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0140-7775</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The pepscan procedure was used to identify betanodavirus B‐cell epitopes recognized by neutralizing mouse monoclonal antibodies (MAbs) and serum samples obtained from sea bass, Dicentrarchus labrax, naturally infected with betanodavirus. Pepscan was performed with a panel of thirty‐four 12‐mer synthetic peptides that mimicked the entire betanodavirus capsid protein. Sea bass serum samples reacted strongly with three regions of the capsid protein comprising amino acid residues 1–32, 91–162 and 181–212. The latter region was also recognized by neutralizing MAbs and coincided with a region of high antigenic propensity identified by an antigen prediction algorithm. These data suggest that a region of the betanodavirus capsid protein spanning amino acid residues 181–212 may represent a neutralization domain that could potentially be used to inform the development of nodavirus vaccines and immunodiagnostic reagents.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000344 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000344 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:8ADB04A13BE598E6911D369B8BB910790FEE7552 |texte= Identification of B‐cell epitopes on the betanodavirus capsid protein }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |