Sequences in the PreC Region of Duck Hepatitis B Virus Affect Pregonomic RNA Accumulation
Identifieur interne : 001038 ( Istex/Corpus ); précédent : 001037; suivant : 001039Sequences in the PreC Region of Duck Hepatitis B Virus Affect Pregonomic RNA Accumulation
Auteurs : Christopher Chang ; Russell C. Hirsch ; Don GanemSource :
- Virology [ 0042-6822 ] ; 1995.
Abstract
Abstract: The pregenomic RNA of hepadnaviruses serves as both the mRNA for the core and polymerase proteins and the RNA template for reverse transcription. We have identified a region in the duck hepatitis B virus pregenomic RNA transcription unit that is critical for the accumulation of this transcript. This 85-nt region, termed α, is located within the preC region; deletion of α results in drastically reduced steady-state levels of pregenomic RNA. This effect is not due to reduction in transcription initiation or to enhancement of premature polyadenylation at the 5′ copy of the viral poly(A) signal. However, this phenotype is suppressed by deletion of a second, larger region (β) located ca. 1 kb downstream. The activity of the α element is tissue- and species-nonspecific; however, it displays absolute orientation-dependence and its activity is influenced by its position within the transcript. Models for its action are discussed.
Url:
DOI: 10.1006/viro.1995.1115
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We have identified a region in the duck hepatitis B virus pregenomic RNA transcription unit that is critical for the accumulation of this transcript. This 85-nt region, termed α, is located within the preC region; deletion of α results in drastically reduced steady-state levels of pregenomic RNA. This effect is not due to reduction in transcription initiation or to enhancement of premature polyadenylation at the 5′ copy of the viral poly(A) signal. However, this phenotype is suppressed by deletion of a second, larger region (β) located ca. 1 kb downstream. The activity of the α element is tissue- and species-nonspecific; however, it displays absolute orientation-dependence and its activity is influenced by its position within the transcript. Models for its action are discussed.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Sequences in the PreC Region of Duck Hepatitis B Virus Affect Pregonomic RNA Accumulation</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Sequences in the PreC Region of Duck Hepatitis B Virus Affect Pregonomic RNA Accumulation</title></titleInfo><name type="personal"><namePart type="given">Christopher</namePart><namePart type="family">Chang</namePart><affiliation>Howard Hughes Medical Institute and Departments of Microbiology and Immunology, Biochemistry and Medicine, University of California Medical Center, Box 0502, San Francisco, California 94143-0502</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Russell C.</namePart><namePart type="family">Hirsch</namePart><affiliation>Howard Hughes Medical Institute and Departments of Microbiology and Immunology, Biochemistry and Medicine, University of California Medical Center, Box 0502, San Francisco, California 94143-0502</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Don</namePart><namePart type="family">Ganem</namePart><affiliation>Howard Hughes Medical Institute and Departments of Microbiology and Immunology, Biochemistry and Medicine, University of California Medical Center, Box 0502, San Francisco, California 94143-0502</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="Short communication" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1995</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The pregenomic RNA of hepadnaviruses serves as both the mRNA for the core and polymerase proteins and the RNA template for reverse transcription. We have identified a region in the duck hepatitis B virus pregenomic RNA transcription unit that is critical for the accumulation of this transcript. This 85-nt region, termed α, is located within the preC region; deletion of α results in drastically reduced steady-state levels of pregenomic RNA. This effect is not due to reduction in transcription initiation or to enhancement of premature polyadenylation at the 5′ copy of the viral poly(A) signal. However, this phenotype is suppressed by deletion of a second, larger region (β) located ca. 1 kb downstream. The activity of the α element is tissue- and species-nonspecific; however, it displays absolute orientation-dependence and its activity is influenced by its position within the transcript. 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