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Effects of db-cAMP and theophylline on Concanavalin A binding site distribution on transformed and protease-treated cell lines

Identifieur interne : 001037 ( Istex/Corpus ); précédent : 001036; suivant : 001038

Effects of db-cAMP and theophylline on Concanavalin A binding site distribution on transformed and protease-treated cell lines

Auteurs : Joan Z. Borysenko ; Th. E. Ukena ; M. J. Karnovsky

Source :

RBID : ISTEX:176A7E8D0AF36D47D0165E0A83C7EB77EE12FB80

English descriptors

Abstract

Abstract: We have examined the effects of N6, O2-dibutyryl 3′: 5′ adenosine-cyclic monophosphate (db-cAMP) and theophylline treatment on the surface distribution of Concanavalin A-binding sites (CABS) on 3T3 cells, SV40-transformed 3T3 cells (SV3T3) and a line of SV40-transformed mouse embryo fibroblasts (VLM). Overnight incubation of VLM cells in medium containing db-cAMP and theophylline completely eliminated the lectin-induced clustering of CABS observed on many transformed cell lines. Similar treatment of SV3T3 cells caused a partial inhibition of clustering. A clone of 3T3 cells that did not normally show lectin-induced clustering could be caused to patch by brief trypsinization. Induction of clustering on these cells after trypsinization was likewise prevented by db-cAMP and theophylline.

Url:
DOI: 10.1016/0014-4827(77)90407-4

Links to Exploration step

ISTEX:176A7E8D0AF36D47D0165E0A83C7EB77EE12FB80

Le document en format XML

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<abstract lang="en">Abstract: We have examined the effects of N6, O2-dibutyryl 3′: 5′ adenosine-cyclic monophosphate (db-cAMP) and theophylline treatment on the surface distribution of Concanavalin A-binding sites (CABS) on 3T3 cells, SV40-transformed 3T3 cells (SV3T3) and a line of SV40-transformed mouse embryo fibroblasts (VLM). Overnight incubation of VLM cells in medium containing db-cAMP and theophylline completely eliminated the lectin-induced clustering of CABS observed on many transformed cell lines. Similar treatment of SV3T3 cells caused a partial inhibition of clustering. A clone of 3T3 cells that did not normally show lectin-induced clustering could be caused to patch by brief trypsinization. Induction of clustering on these cells after trypsinization was likewise prevented by db-cAMP and theophylline.</abstract>
<note>This study was supported by USPHS grants CA 17328 and CA 14732 from the NCI.</note>
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