Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR.
Identifieur interne : 001D73 ( Ncbi/Curation ); précédent : 001D72; suivant : 001D74Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR.
Auteurs : P. Kanjanavas [Thaïlande] ; P. Tan-Ariya ; P. Khawsak ; A. Pakpitcharoen ; S. Phantana ; K. ChansiriSource :
- Molecular and cellular probes [ 0890-8508 ] ; 2005.
Descripteurs français
- KwdFr :
- ADN (génétique), Animaux, Facteurs temps, Filariose lymphatique (parasitologie), Filariose lymphatique (sang), Génome, Porteur sain (parasitologie), Prélèvement sanguin, Réaction de polymérisation en chaîne (), Tissu lymphoïde (parasitologie), Wuchereria bancrofti (génétique), Wuchereria bancrofti (isolement et purification).
- MESH :
- génétique : ADN, Wuchereria bancrofti.
- isolement et purification : Wuchereria bancrofti.
- parasitologie : Filariose lymphatique, Porteur sain, Tissu lymphoïde.
- sang : Filariose lymphatique.
- Animaux, Facteurs temps, Génome, Prélèvement sanguin, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- Animals, Blood Specimen Collection, Carrier State (parasitology), DNA (genetics), Elephantiasis, Filarial (blood), Elephantiasis, Filarial (parasitology), Genome, Lymphoid Tissue (parasitology), Polymerase Chain Reaction (methods), Time Factors, Wuchereria bancrofti (genetics), Wuchereria bancrofti (isolation & purification).
- MESH :
- chemical , genetics : DNA.
- blood : Elephantiasis, Filarial.
- genetics : Wuchereria bancrofti.
- isolation & purification : Wuchereria bancrofti.
- methods : Polymerase Chain Reaction.
- parasitology : Carrier State, Elephantiasis, Filarial, Lymphoid Tissue.
- Animals, Blood Specimen Collection, Genome, Time Factors.
Abstract
The semi-nested PCR was conducted for detection of Wuchereria bancrofti in patients' blood. The primers were designed based on the repetitive DNA sequences of the parasite. The results demonstrated that the semi-nested PCR could detect as little as 0.001 fg of parasite DNA. In addition, the primers showed no PCR amplification from human and other hemoparasites such as Brugia malayi, Plasmodium falciparum and P. vivax DNAs. This technique was used for detection of 18 W. bancrofti infected blood samples with a long-term storage, the data revealed that all samples were positive. The results obtained from this study clearly indicated that the semi-nested PCR is specific, sensitive, and suitable for detection of the disease carriers.
DOI: 10.1016/j.mcp.2004.11.003
PubMed: 15797816
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pubmed:15797816Le document en format XML
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Khawsak</name></author><author><name sortKey="Pakpitcharoen, A" sort="Pakpitcharoen, A" uniqKey="Pakpitcharoen A" first="A" last="Pakpitcharoen">A. Pakpitcharoen</name></author><author><name sortKey="Phantana, S" sort="Phantana, S" uniqKey="Phantana S" first="S" last="Phantana">S. Phantana</name></author><author><name sortKey="Chansiri, K" sort="Chansiri, K" uniqKey="Chansiri K" first="K" last="Chansiri">K. Chansiri</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15797816</idno><idno type="pmid">15797816</idno><idno type="doi">10.1016/j.mcp.2004.11.003</idno><idno type="wicri:Area/PubMed/Corpus">003D29</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003D29</idno><idno type="wicri:Area/PubMed/Curation">003D29</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003D29</idno><idno type="wicri:Area/PubMed/Checkpoint">003D29</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003D29</idno><idno type="wicri:Area/Ncbi/Merge">001D73</idno><idno type="wicri:Area/Ncbi/Curation">001D73</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR.</title><author><name sortKey="Kanjanavas, P" sort="Kanjanavas, P" uniqKey="Kanjanavas P" first="P" last="Kanjanavas">P. Kanjanavas</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110</wicri:regionArea><wicri:noRegion>Bangkok 10110</wicri:noRegion></affiliation></author><author><name sortKey="Tan Ariya, P" sort="Tan Ariya, P" uniqKey="Tan Ariya P" first="P" last="Tan-Ariya">P. Tan-Ariya</name></author><author><name sortKey="Khawsak, P" sort="Khawsak, P" uniqKey="Khawsak P" first="P" last="Khawsak">P. Khawsak</name></author><author><name sortKey="Pakpitcharoen, A" sort="Pakpitcharoen, A" uniqKey="Pakpitcharoen A" first="A" last="Pakpitcharoen">A. Pakpitcharoen</name></author><author><name sortKey="Phantana, S" sort="Phantana, S" uniqKey="Phantana S" first="S" last="Phantana">S. Phantana</name></author><author><name sortKey="Chansiri, K" sort="Chansiri, K" uniqKey="Chansiri K" first="K" last="Chansiri">K. Chansiri</name></author></analytic><series><title level="j">Molecular and cellular probes</title><idno type="ISSN">0890-8508</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blood Specimen Collection</term><term>Carrier State (parasitology)</term><term>DNA (genetics)</term><term>Elephantiasis, Filarial (blood)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Genome</term><term>Lymphoid Tissue (parasitology)</term><term>Polymerase Chain Reaction (methods)</term><term>Time Factors</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (génétique)</term><term>Animaux</term><term>Facteurs temps</term><term>Filariose lymphatique (parasitologie)</term><term>Filariose lymphatique (sang)</term><term>Génome</term><term>Porteur sain (parasitologie)</term><term>Prélèvement sanguin</term><term>Réaction de polymérisation en chaîne ()</term><term>Tissu lymphoïde (parasitologie)</term><term>Wuchereria bancrofti (génétique)</term><term>Wuchereria bancrofti (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term><term>Porteur sain</term><term>Tissu lymphoïde</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Carrier State</term><term>Elephantiasis, Filarial</term><term>Lymphoid Tissue</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blood Specimen Collection</term><term>Genome</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Facteurs temps</term><term>Génome</term><term>Prélèvement sanguin</term><term>Réaction de polymérisation en chaîne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The semi-nested PCR was conducted for detection of Wuchereria bancrofti in patients' blood. The primers were designed based on the repetitive DNA sequences of the parasite. The results demonstrated that the semi-nested PCR could detect as little as 0.001 fg of parasite DNA. In addition, the primers showed no PCR amplification from human and other hemoparasites such as Brugia malayi, Plasmodium falciparum and P. vivax DNAs. This technique was used for detection of 18 W. bancrofti infected blood samples with a long-term storage, the data revealed that all samples were positive. The results obtained from this study clearly indicated that the semi-nested PCR is specific, sensitive, and suitable for detection of the disease carriers.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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