Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis
Identifieur interne : 001D72 ( Ncbi/Curation ); précédent : 001D71; suivant : 001D73Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis
Auteurs : Xiao-Nan Cui ; Jian-Wu Tang ; Li Hou ; Bo Song ; Li Li ; Ji-Wei LiuSource :
- World Journal of Gastroenterology : WJG [ 1007-9327 ] ; 2005.
Abstract
AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.
RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.
CONCLUSION: SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.
Url:
DOI: 10.3748/wjg.v11.i12.1837
PubMed: 15793877
PubMed Central: 4305887
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001E93
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :001E92
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :003D32
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001D72
Links to Exploration step
PMC:4305887Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis</title><author><name sortKey="Cui, Xiao Nan" sort="Cui, Xiao Nan" uniqKey="Cui X" first="Xiao-Nan" last="Cui">Xiao-Nan Cui</name></author><author><name sortKey="Tang, Jian Wu" sort="Tang, Jian Wu" uniqKey="Tang J" first="Jian-Wu" last="Tang">Jian-Wu Tang</name></author><author><name sortKey="Hou, Li" sort="Hou, Li" uniqKey="Hou L" first="Li" last="Hou">Li Hou</name></author><author><name sortKey="Song, Bo" sort="Song, Bo" uniqKey="Song B" first="Bo" last="Song">Bo Song</name></author><author><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name></author><author><name sortKey="Liu, Ji Wei" sort="Liu, Ji Wei" uniqKey="Liu J" first="Ji-Wei" last="Liu">Ji-Wei Liu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15793877</idno><idno type="pmc">4305887</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4305887</idno><idno type="RBID">PMC:4305887</idno><idno type="doi">10.3748/wjg.v11.i12.1837</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">001E93</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001E93</idno><idno type="wicri:Area/Pmc/Curation">001E92</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001E92</idno><idno type="wicri:Area/Pmc/Checkpoint">003D32</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003D32</idno><idno type="wicri:Area/Ncbi/Merge">001D72</idno><idno type="wicri:Area/Ncbi/Curation">001D72</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis</title><author><name sortKey="Cui, Xiao Nan" sort="Cui, Xiao Nan" uniqKey="Cui X" first="Xiao-Nan" last="Cui">Xiao-Nan Cui</name></author><author><name sortKey="Tang, Jian Wu" sort="Tang, Jian Wu" uniqKey="Tang J" first="Jian-Wu" last="Tang">Jian-Wu Tang</name></author><author><name sortKey="Hou, Li" sort="Hou, Li" uniqKey="Hou L" first="Li" last="Hou">Li Hou</name></author><author><name sortKey="Song, Bo" sort="Song, Bo" uniqKey="Song B" first="Bo" last="Song">Bo Song</name></author><author><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name></author><author><name sortKey="Liu, Ji Wei" sort="Liu, Ji Wei" uniqKey="Liu J" first="Ji-Wei" last="Liu">Ji-Wei Liu</name></author></analytic><series><title level="j">World Journal of Gastroenterology : WJG</title><idno type="ISSN">1007-9327</idno><idno type="eISSN">2219-2840</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.</p><p>METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.</p><p>RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.</p><p>CONCLUSION: SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.</p></div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001D72 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 001D72 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Ncbi
|étape= Curation
|type= RBID
|clé= PMC:4305887
|texte= Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:15793877" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |