Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR.
Identifieur interne : 003D29 ( PubMed/Corpus ); précédent : 003D28; suivant : 003D30Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR.
Auteurs : P. Kanjanavas ; P. Tan-Ariya ; P. Khawsak ; A. Pakpitcharoen ; S. Phantana ; K. ChansiriSource :
- Molecular and cellular probes [ 0890-8508 ] ; 2005.
English descriptors
- KwdEn :
- Animals, Blood Specimen Collection, Carrier State (parasitology), DNA (genetics), Elephantiasis, Filarial (blood), Elephantiasis, Filarial (parasitology), Genome, Lymphoid Tissue (parasitology), Polymerase Chain Reaction (methods), Time Factors, Wuchereria bancrofti (genetics), Wuchereria bancrofti (isolation & purification).
- MESH :
- chemical , genetics : DNA.
- blood : Elephantiasis, Filarial.
- genetics : Wuchereria bancrofti.
- isolation & purification : Wuchereria bancrofti.
- methods : Polymerase Chain Reaction.
- parasitology : Carrier State, Elephantiasis, Filarial, Lymphoid Tissue.
- Animals, Blood Specimen Collection, Genome, Time Factors.
Abstract
The semi-nested PCR was conducted for detection of Wuchereria bancrofti in patients' blood. The primers were designed based on the repetitive DNA sequences of the parasite. The results demonstrated that the semi-nested PCR could detect as little as 0.001 fg of parasite DNA. In addition, the primers showed no PCR amplification from human and other hemoparasites such as Brugia malayi, Plasmodium falciparum and P. vivax DNAs. This technique was used for detection of 18 W. bancrofti infected blood samples with a long-term storage, the data revealed that all samples were positive. The results obtained from this study clearly indicated that the semi-nested PCR is specific, sensitive, and suitable for detection of the disease carriers.
DOI: 10.1016/j.mcp.2004.11.003
PubMed: 15797816
Links to Exploration step
pubmed:15797816Le document en format XML
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Pakpitcharoen</name></author><author><name sortKey="Phantana, S" sort="Phantana, S" uniqKey="Phantana S" first="S" last="Phantana">S. Phantana</name></author><author><name sortKey="Chansiri, K" sort="Chansiri, K" uniqKey="Chansiri K" first="K" last="Chansiri">K. Chansiri</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15797816</idno><idno type="pmid">15797816</idno><idno type="doi">10.1016/j.mcp.2004.11.003</idno><idno type="wicri:Area/PubMed/Corpus">003D29</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003D29</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR.</title><author><name sortKey="Kanjanavas, P" sort="Kanjanavas, P" uniqKey="Kanjanavas P" first="P" last="Kanjanavas">P. Kanjanavas</name><affiliation><nlm:affiliation>Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand.</nlm:affiliation></affiliation></author><author><name sortKey="Tan Ariya, P" sort="Tan Ariya, P" uniqKey="Tan Ariya P" first="P" last="Tan-Ariya">P. Tan-Ariya</name></author><author><name sortKey="Khawsak, P" sort="Khawsak, P" uniqKey="Khawsak P" first="P" last="Khawsak">P. Khawsak</name></author><author><name sortKey="Pakpitcharoen, A" sort="Pakpitcharoen, A" uniqKey="Pakpitcharoen A" first="A" last="Pakpitcharoen">A. Pakpitcharoen</name></author><author><name sortKey="Phantana, S" sort="Phantana, S" uniqKey="Phantana S" first="S" last="Phantana">S. Phantana</name></author><author><name sortKey="Chansiri, K" sort="Chansiri, K" uniqKey="Chansiri K" first="K" last="Chansiri">K. Chansiri</name></author></analytic><series><title level="j">Molecular and cellular probes</title><idno type="ISSN">0890-8508</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blood Specimen Collection</term><term>Carrier State (parasitology)</term><term>DNA (genetics)</term><term>Elephantiasis, Filarial (blood)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Genome</term><term>Lymphoid Tissue (parasitology)</term><term>Polymerase Chain Reaction (methods)</term><term>Time Factors</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (isolation & purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Carrier State</term><term>Elephantiasis, Filarial</term><term>Lymphoid Tissue</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blood Specimen Collection</term><term>Genome</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The semi-nested PCR was conducted for detection of Wuchereria bancrofti in patients' blood. The primers were designed based on the repetitive DNA sequences of the parasite. The results demonstrated that the semi-nested PCR could detect as little as 0.001 fg of parasite DNA. In addition, the primers showed no PCR amplification from human and other hemoparasites such as Brugia malayi, Plasmodium falciparum and P. vivax DNAs. This technique was used for detection of 18 W. bancrofti infected blood samples with a long-term storage, the data revealed that all samples were positive. The results obtained from this study clearly indicated that the semi-nested PCR is specific, sensitive, and suitable for detection of the disease carriers.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15797816</PMID><DateCreated><Year>2005</Year><Month>03</Month><Day>30</Day></DateCreated><DateCompleted><Year>2005</Year><Month>07</Month><Day>15</Day></DateCompleted><DateRevised><Year>2005</Year><Month>03</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0890-8508</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>3</Issue><PubDate><Year>2005</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Molecular and cellular probes</Title><ISOAbbreviation>Mol. Cell. Probes</ISOAbbreviation></Journal><ArticleTitle>Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR.</ArticleTitle><Pagination><MedlinePgn>169-72</MedlinePgn></Pagination><Abstract><AbstractText>The semi-nested PCR was conducted for detection of Wuchereria bancrofti in patients' blood. The primers were designed based on the repetitive DNA sequences of the parasite. The results demonstrated that the semi-nested PCR could detect as little as 0.001 fg of parasite DNA. In addition, the primers showed no PCR amplification from human and other hemoparasites such as Brugia malayi, Plasmodium falciparum and P. vivax DNAs. This technique was used for detection of 18 W. bancrofti infected blood samples with a long-term storage, the data revealed that all samples were positive. The results obtained from this study clearly indicated that the semi-nested PCR is specific, sensitive, and suitable for detection of the disease carriers.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kanjanavas</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok 10110, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tan-ariya</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Khawsak</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Pakpitcharoen</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Phantana</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Chansiri</LastName><ForeName>K</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Cell Probes</MedlineTA><NlmUniqueID>8709751</NlmUniqueID><ISSNLinking>0890-8508</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001800" MajorTopicYN="N">Blood Specimen Collection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002353" MajorTopicYN="N">Carrier State</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016678" MajorTopicYN="N">Genome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008221" MajorTopicYN="N">Lymphoid Tissue</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>08</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>11</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>3</Month><Day>31</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>7</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>3</Month><Day>31</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15797816</ArticleId><ArticleId IdType="pii">S0890-8508(04)00104-5</ArticleId><ArticleId IdType="doi">10.1016/j.mcp.2004.11.003</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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