Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis
Identifieur interne : 001E92 ( Pmc/Curation ); précédent : 001E91; suivant : 001E93Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis
Auteurs : Xiao-Nan Cui ; Jian-Wu Tang ; Li Hou ; Bo Song ; Li Li ; Ji-Wei LiuSource :
- World Journal of Gastroenterology : WJG [ 1007-9327 ] ; 2005.
Abstract
AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.
RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.
CONCLUSION: SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.
Url:
DOI: 10.3748/wjg.v11.i12.1837
PubMed: 15793877
PubMed Central: 4305887
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<front><journal-meta><journal-id journal-id-type="nlm-ta">World J Gastroenterol</journal-id><journal-id journal-id-type="iso-abbrev">World J. Gastroenterol</journal-id><journal-id journal-id-type="publisher-id">WJG</journal-id><journal-title-group><journal-title>World Journal of Gastroenterology : WJG</journal-title></journal-title-group><issn pub-type="ppub">1007-9327</issn><issn pub-type="epub">2219-2840</issn><publisher><publisher-name>Baishideng Publishing Group Inc</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15793877</article-id><article-id pub-id-type="pmc">4305887</article-id><article-id pub-id-type="other">jWJG.v11.i12.pg1837</article-id><article-id pub-id-type="doi">10.3748/wjg.v11.i12.1837</article-id><article-categories><subj-group subj-group-type="heading"><subject>Brief Reports</subject></subj-group></article-categories><title-group><article-title>Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Cui</surname><given-names>Xiao-Nan</given-names></name></contrib><contrib contrib-type="author"><name><surname>Tang</surname><given-names>Jian-Wu</given-names></name></contrib><contrib contrib-type="author"><name><surname>Hou</surname><given-names>Li</given-names></name></contrib><contrib contrib-type="author"><name><surname>Song</surname><given-names>Bo</given-names></name></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Li</given-names></name></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Ji-Wei</given-names></name></contrib><aff>Xiao-Nan Cui, Li Li, Ji-Wei Liu, Department of Oncology, the 1st Affilitate Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China</aff><aff>Jian-Wu Tang, Li Hou, Bo Song, Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China</aff></contrib-group><author-notes><fn><p>Author contributions: All authors contributed equally to the work.</p><p>Correspondence to: Jian-Wu Tang, Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China. <email>jianwutang@163.net</email></p><p>Telephone: +86-411-4720021</p></fn></author-notes><pub-date pub-type="ppub"><day>28</day><month>3</month><year>2005</year></pub-date><pub-date pub-type="epub"><day>28</day><month>3</month><year>2005</year></pub-date><volume>11</volume><issue>12</issue><fpage>1837</fpage><lpage>1842</lpage><history><date date-type="received"><day>3</day><month>2</month><year>2004</year></date><date date-type="rev-recd"><day>4</day><month>2</month><year>2004</year></date><date date-type="accepted"><day>21</day><month>2</month><year>2004</year></date></history><permissions><copyright-statement>©2005 Baishideng Publishing Group Inc. All rights reserved.</copyright-statement><copyright-year>2005</copyright-year></permissions><abstract><p>AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.</p><p>METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.</p><p>RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.</p><p>CONCLUSION: SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.</p></abstract><kwd-group><kwd>Suppression subtracted hybridization</kwd><kwd>Liver neoplasm</kwd><kwd>Lymphogenous metastasis</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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