Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis
Identifieur interne : 001E92 ( Pmc/Curation ); précédent : 001E91; suivant : 001E93Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis
Auteurs : Xiao-Nan Cui ; Jian-Wu Tang ; Li Hou ; Bo Song ; Li Li ; Ji-Wei LiuSource :
- World Journal of Gastroenterology : WJG [ 1007-9327 ] ; 2005.
Abstract
AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.
RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.
CONCLUSION: SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.
Url:
DOI: 10.3748/wjg.v11.i12.1837
PubMed: 15793877
PubMed Central: 4305887
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis</title>
<author><name sortKey="Cui, Xiao Nan" sort="Cui, Xiao Nan" uniqKey="Cui X" first="Xiao-Nan" last="Cui">Xiao-Nan Cui</name>
</author>
<author><name sortKey="Tang, Jian Wu" sort="Tang, Jian Wu" uniqKey="Tang J" first="Jian-Wu" last="Tang">Jian-Wu Tang</name>
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<author><name sortKey="Hou, Li" sort="Hou, Li" uniqKey="Hou L" first="Li" last="Hou">Li Hou</name>
</author>
<author><name sortKey="Song, Bo" sort="Song, Bo" uniqKey="Song B" first="Bo" last="Song">Bo Song</name>
</author>
<author><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name>
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<author><name sortKey="Liu, Ji Wei" sort="Liu, Ji Wei" uniqKey="Liu J" first="Ji-Wei" last="Liu">Ji-Wei Liu</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis</title>
<author><name sortKey="Cui, Xiao Nan" sort="Cui, Xiao Nan" uniqKey="Cui X" first="Xiao-Nan" last="Cui">Xiao-Nan Cui</name>
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<author><name sortKey="Tang, Jian Wu" sort="Tang, Jian Wu" uniqKey="Tang J" first="Jian-Wu" last="Tang">Jian-Wu Tang</name>
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<author><name sortKey="Hou, Li" sort="Hou, Li" uniqKey="Hou L" first="Li" last="Hou">Li Hou</name>
</author>
<author><name sortKey="Song, Bo" sort="Song, Bo" uniqKey="Song B" first="Bo" last="Song">Bo Song</name>
</author>
<author><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name>
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<author><name sortKey="Liu, Ji Wei" sort="Liu, Ji Wei" uniqKey="Liu J" first="Ji-Wei" last="Liu">Ji-Wei Liu</name>
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</analytic>
<series><title level="j">World Journal of Gastroenterology : WJG</title>
<idno type="ISSN">1007-9327</idno>
<idno type="eISSN">2219-2840</idno>
<imprint><date when="2005">2005</date>
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<front><div type="abstract" xml:lang="en"><p>AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.</p>
<p>METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.</p>
<p>RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.</p>
<p>CONCLUSION: SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.</p>
</div>
</front>
</TEI>
<pmc article-type="brief-report"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">World J Gastroenterol</journal-id>
<journal-id journal-id-type="iso-abbrev">World J. Gastroenterol</journal-id>
<journal-id journal-id-type="publisher-id">WJG</journal-id>
<journal-title-group><journal-title>World Journal of Gastroenterology : WJG</journal-title>
</journal-title-group>
<issn pub-type="ppub">1007-9327</issn>
<issn pub-type="epub">2219-2840</issn>
<publisher><publisher-name>Baishideng Publishing Group Inc</publisher-name>
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<article-meta><article-id pub-id-type="pmid">15793877</article-id>
<article-id pub-id-type="pmc">4305887</article-id>
<article-id pub-id-type="other">jWJG.v11.i12.pg1837</article-id>
<article-id pub-id-type="doi">10.3748/wjg.v11.i12.1837</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Brief Reports</subject>
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<title-group><article-title>Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Cui</surname>
<given-names>Xiao-Nan</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Tang</surname>
<given-names>Jian-Wu</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Hou</surname>
<given-names>Li</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Song</surname>
<given-names>Bo</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Li</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Liu</surname>
<given-names>Ji-Wei</given-names>
</name>
</contrib>
<aff>Xiao-Nan Cui, Li Li, Ji-Wei Liu, Department of Oncology, the 1st Affilitate Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China</aff>
<aff>Jian-Wu Tang, Li Hou, Bo Song, Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China</aff>
</contrib-group>
<author-notes><fn><p>Author contributions: All authors contributed equally to the work.</p>
<p>Correspondence to: Jian-Wu Tang, Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China. <email>jianwutang@163.net</email>
</p>
<p>Telephone: +86-411-4720021</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><day>28</day>
<month>3</month>
<year>2005</year>
</pub-date>
<pub-date pub-type="epub"><day>28</day>
<month>3</month>
<year>2005</year>
</pub-date>
<volume>11</volume>
<issue>12</issue>
<fpage>1837</fpage>
<lpage>1842</lpage>
<history><date date-type="received"><day>3</day>
<month>2</month>
<year>2004</year>
</date>
<date date-type="rev-recd"><day>4</day>
<month>2</month>
<year>2004</year>
</date>
<date date-type="accepted"><day>21</day>
<month>2</month>
<year>2004</year>
</date>
</history>
<permissions><copyright-statement>©2005 Baishideng Publishing Group Inc. All rights reserved.</copyright-statement>
<copyright-year>2005</copyright-year>
</permissions>
<abstract><p>AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.</p>
<p>METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.</p>
<p>RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.</p>
<p>CONCLUSION: SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.</p>
</abstract>
<kwd-group><kwd>Suppression subtracted hybridization</kwd>
<kwd>Liver neoplasm</kwd>
<kwd>Lymphogenous metastasis</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
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