Biomaterial Guides for Lymphatic Endothelial Cell Alignment and Migration
Identifieur interne : 003F09 ( Ncbi/Checkpoint ); précédent : 003F08; suivant : 003F10Biomaterial Guides for Lymphatic Endothelial Cell Alignment and Migration
Auteurs : Echoe M. Bouta [États-Unis] ; Connor W. Mccarthy [États-Unis] ; Alexander Keim [États-Unis] ; Han Bing Wang [États-Unis] ; Ryan J. Gilbert [États-Unis] ; Jeremy Goldman [États-Unis]Source :
- Acta biomaterialia [ 1742-7061 ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical : Biocompatible Materials.
- cytology : Endothelium, Lymphatic Vessels.
- ultrastructure : Lymphatic Vessels.
- Cell Movement, Humans, Microscopy, Electron, Scanning.
Abstract
Axillary dissection during breast cancer surgery produces extensive lymphatic vessel damage that often leads to life-long secondary lymphedema of the arm. We have developed a biodegradable material conduit for lymphatic vessel reconstruction where fibers electrospun along the conduit lumen promote endothelial cell alignment and migration, in vitro. The diameter and density of the electrospun fibers were optimized for cell migration and direction on 2-dimensional substrates by seeding human lymphatic endothelial cells (LECs) onto aligned fibers of varying diameters and densities, randomly oriented fibers, and film substrates with no fibers. We found that LECs became aligned in the fiber direction, with cells seeded on the randomly oriented fibers becoming oriented in random directions, whereas cells seeded on the highly aligned fibers became highly aligned. Cell migration was dependent upon fiber alignment and density, with optimal migration found on 1300 nm diameter aligned fibers of low density. Blood endothelial cells seeded on the fibers exhibited similar behavior as the LECs. Fiber alignment was preserved upon rolling the 2-dimensional substrate into the tubular geometry of a lymphatic vessel. The data suggest that aligned electrospun fibers may promote endothelial migration across the conduit in a manner that is independent of lymphatic growth factors.
Url:
DOI: 10.1016/j.actbio.2010.10.016
PubMed: 20974299
PubMed Central: 3031728
Affiliations:
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Bouta</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931-1295</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Michigan</region></placeName><wicri:cityArea>Department of Biomedical Engineering, Michigan Technological University, Houghton</wicri:cityArea></affiliation></author><author><name sortKey="Mccarthy, Connor W" sort="Mccarthy, Connor W" uniqKey="Mccarthy C" first="Connor W." last="Mccarthy">Connor W. Mccarthy</name><affiliation wicri:level="2"><nlm:aff id="A2">Regeneration and Repair Laboratory, Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931-1295</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Michigan</region></placeName><wicri:cityArea>Regeneration and Repair Laboratory, Department of Biomedical Engineering, Michigan Technological University, Houghton</wicri:cityArea></affiliation></author><author><name sortKey="Keim, Alexander" sort="Keim, Alexander" uniqKey="Keim A" first="Alexander" last="Keim">Alexander Keim</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931-1295</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Michigan</region></placeName><wicri:cityArea>Department of Biomedical Engineering, Michigan Technological University, Houghton</wicri:cityArea></affiliation></author><author><name sortKey="Wang, Han Bing" sort="Wang, Han Bing" uniqKey="Wang H" first="Han Bing" last="Wang">Han Bing Wang</name><affiliation wicri:level="2"><nlm:aff id="A2">Regeneration and Repair Laboratory, Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931-1295</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Michigan</region></placeName><wicri:cityArea>Regeneration and Repair Laboratory, Department of Biomedical Engineering, Michigan Technological University, Houghton</wicri:cityArea></affiliation></author><author><name sortKey="Gilbert, Ryan J" sort="Gilbert, Ryan J" uniqKey="Gilbert R" first="Ryan J." last="Gilbert">Ryan J. 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We have developed a biodegradable material conduit for lymphatic vessel reconstruction where fibers electrospun along the conduit lumen promote endothelial cell alignment and migration, in vitro. The diameter and density of the electrospun fibers were optimized for cell migration and direction on 2-dimensional substrates by seeding human lymphatic endothelial cells (LECs) onto aligned fibers of varying diameters and densities, randomly oriented fibers, and film substrates with no fibers. We found that LECs became aligned in the fiber direction, with cells seeded on the randomly oriented fibers becoming oriented in random directions, whereas cells seeded on the highly aligned fibers became highly aligned. Cell migration was dependent upon fiber alignment and density, with optimal migration found on 1300 nm diameter aligned fibers of low density. Blood endothelial cells seeded on the fibers exhibited similar behavior as the LECs. Fiber alignment was preserved upon rolling the 2-dimensional substrate into the tubular geometry of a lymphatic vessel. The data suggest that aligned electrospun fibers may promote endothelial migration across the conduit in a manner that is independent of lymphatic growth factors.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region></list><tree><country name="États-Unis"><region name="Michigan"><name sortKey="Bouta, Echoe M" sort="Bouta, Echoe M" uniqKey="Bouta E" first="Echoe M." last="Bouta">Echoe M. Bouta</name></region><name sortKey="Gilbert, Ryan J" sort="Gilbert, Ryan J" uniqKey="Gilbert R" first="Ryan J." last="Gilbert">Ryan J. Gilbert</name><name sortKey="Goldman, Jeremy" sort="Goldman, Jeremy" uniqKey="Goldman J" first="Jeremy" last="Goldman">Jeremy Goldman</name><name sortKey="Keim, Alexander" sort="Keim, Alexander" uniqKey="Keim A" first="Alexander" last="Keim">Alexander Keim</name><name sortKey="Mccarthy, Connor W" sort="Mccarthy, Connor W" uniqKey="Mccarthy C" first="Connor W." last="Mccarthy">Connor W. Mccarthy</name><name sortKey="Wang, Han Bing" sort="Wang, Han Bing" uniqKey="Wang H" first="Han Bing" last="Wang">Han Bing Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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