Biomaterial Guides for Lymphatic Endothelial Cell Alignment and Migration
Identifieur interne : 002816 ( Pmc/Corpus ); précédent : 002815; suivant : 002817Biomaterial Guides for Lymphatic Endothelial Cell Alignment and Migration
Auteurs : Echoe M. Bouta ; Connor W. Mccarthy ; Alexander Keim ; Han Bing Wang ; Ryan J. Gilbert ; Jeremy GoldmanSource :
- Acta biomaterialia [ 1742-7061 ] ; 2010.
Abstract
Axillary dissection during breast cancer surgery produces extensive lymphatic vessel damage that often leads to life-long secondary lymphedema of the arm. We have developed a biodegradable material conduit for lymphatic vessel reconstruction where fibers electrospun along the conduit lumen promote endothelial cell alignment and migration, in vitro. The diameter and density of the electrospun fibers were optimized for cell migration and direction on 2-dimensional substrates by seeding human lymphatic endothelial cells (LECs) onto aligned fibers of varying diameters and densities, randomly oriented fibers, and film substrates with no fibers. We found that LECs became aligned in the fiber direction, with cells seeded on the randomly oriented fibers becoming oriented in random directions, whereas cells seeded on the highly aligned fibers became highly aligned. Cell migration was dependent upon fiber alignment and density, with optimal migration found on 1300 nm diameter aligned fibers of low density. Blood endothelial cells seeded on the fibers exhibited similar behavior as the LECs. Fiber alignment was preserved upon rolling the 2-dimensional substrate into the tubular geometry of a lymphatic vessel. The data suggest that aligned electrospun fibers may promote endothelial migration across the conduit in a manner that is independent of lymphatic growth factors.
Url:
DOI: 10.1016/j.actbio.2010.10.016
PubMed: 20974299
PubMed Central: 3031728
Links to Exploration step
PMC:3031728Le document en format XML
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<affiliation><nlm:aff id="A1">Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931-1295</nlm:aff>
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<author><name sortKey="Mccarthy, Connor W" sort="Mccarthy, Connor W" uniqKey="Mccarthy C" first="Connor W." last="Mccarthy">Connor W. Mccarthy</name>
<affiliation><nlm:aff id="A2">Regeneration and Repair Laboratory, Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931-1295</nlm:aff>
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<author><name sortKey="Keim, Alexander" sort="Keim, Alexander" uniqKey="Keim A" first="Alexander" last="Keim">Alexander Keim</name>
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<author><name sortKey="Wang, Han Bing" sort="Wang, Han Bing" uniqKey="Wang H" first="Han Bing" last="Wang">Han Bing Wang</name>
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<author><name sortKey="Gilbert, Ryan J" sort="Gilbert, Ryan J" uniqKey="Gilbert R" first="Ryan J." last="Gilbert">Ryan J. Gilbert</name>
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<author><name sortKey="Goldman, Jeremy" sort="Goldman, Jeremy" uniqKey="Goldman J" first="Jeremy" last="Goldman">Jeremy Goldman</name>
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<series><title level="j">Acta biomaterialia</title>
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<front><div type="abstract" xml:lang="en"><p id="P2">Axillary dissection during breast cancer surgery produces extensive lymphatic vessel damage that often leads to life-long secondary lymphedema of the arm. We have developed a biodegradable material conduit for lymphatic vessel reconstruction where fibers electrospun along the conduit lumen promote endothelial cell alignment and migration, in vitro. The diameter and density of the electrospun fibers were optimized for cell migration and direction on 2-dimensional substrates by seeding human lymphatic endothelial cells (LECs) onto aligned fibers of varying diameters and densities, randomly oriented fibers, and film substrates with no fibers. We found that LECs became aligned in the fiber direction, with cells seeded on the randomly oriented fibers becoming oriented in random directions, whereas cells seeded on the highly aligned fibers became highly aligned. Cell migration was dependent upon fiber alignment and density, with optimal migration found on 1300 nm diameter aligned fibers of low density. Blood endothelial cells seeded on the fibers exhibited similar behavior as the LECs. Fiber alignment was preserved upon rolling the 2-dimensional substrate into the tubular geometry of a lymphatic vessel. The data suggest that aligned electrospun fibers may promote endothelial migration across the conduit in a manner that is independent of lymphatic growth factors.</p>
</div>
</front>
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<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101233144</journal-id>
<journal-id journal-id-type="pubmed-jr-id">32834</journal-id>
<journal-id journal-id-type="nlm-ta">Acta Biomater</journal-id>
<journal-title>Acta biomaterialia</journal-title>
<issn pub-type="ppub">1742-7061</issn>
<issn pub-type="epub">1878-7568</issn>
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<article-meta><article-id pub-id-type="pmid">20974299</article-id>
<article-id pub-id-type="pmc">3031728</article-id>
<article-id pub-id-type="doi">10.1016/j.actbio.2010.10.016</article-id>
<article-id pub-id-type="manuscript">NIHMS248768</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Biomaterial Guides for Lymphatic Endothelial Cell Alignment and Migration</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Bouta</surname>
<given-names>Echoe M.</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="author-notes" rid="FN2">*</xref>
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<contrib contrib-type="author"><name><surname>McCarthy</surname>
<given-names>Connor W.</given-names>
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<xref ref-type="aff" rid="A2">2</xref>
<xref ref-type="author-notes" rid="FN2">*</xref>
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<contrib contrib-type="author"><name><surname>Keim</surname>
<given-names>Alexander</given-names>
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<xref ref-type="aff" rid="A1">1</xref>
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<contrib contrib-type="author"><name><surname>Wang</surname>
<given-names>Han Bing</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gilbert</surname>
<given-names>Ryan J.</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Goldman</surname>
<given-names>Jeremy</given-names>
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<xref ref-type="aff" rid="A1">1</xref>
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<aff id="A1"><label>1</label>
Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931-1295</aff>
<aff id="A2"><label>2</label>
Regeneration and Repair Laboratory, Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931-1295</aff>
<author-notes><corresp id="CR1">Address for Correspondence: Jeremy Goldman, Ph.D., Associate Professor, Biomedical Engineering Department, Michigan Technological University, Houghton, MI 49931 USA, ph: (906) 487-2851, Fax: (906) 487-1717, <email>jgoldman@mtu.edu</email>
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<fn fn-type="equal" id="FN2"><label>*</label>
<p id="P1">Authors contributed equal work</p>
</fn>
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<pub-date pub-type="nihms-submitted"><day>28</day>
<month>10</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="epub"><day>23</day>
<month>10</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="ppub"><month>3</month>
<year>2011</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>1</day>
<month>3</month>
<year>2012</year>
</pub-date>
<volume>7</volume>
<issue>3</issue>
<fpage>1104</fpage>
<lpage>1113</lpage>
<permissions><copyright-statement>© 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.</copyright-statement>
<copyright-year>2010</copyright-year>
</permissions>
<abstract><p id="P2">Axillary dissection during breast cancer surgery produces extensive lymphatic vessel damage that often leads to life-long secondary lymphedema of the arm. We have developed a biodegradable material conduit for lymphatic vessel reconstruction where fibers electrospun along the conduit lumen promote endothelial cell alignment and migration, in vitro. The diameter and density of the electrospun fibers were optimized for cell migration and direction on 2-dimensional substrates by seeding human lymphatic endothelial cells (LECs) onto aligned fibers of varying diameters and densities, randomly oriented fibers, and film substrates with no fibers. We found that LECs became aligned in the fiber direction, with cells seeded on the randomly oriented fibers becoming oriented in random directions, whereas cells seeded on the highly aligned fibers became highly aligned. Cell migration was dependent upon fiber alignment and density, with optimal migration found on 1300 nm diameter aligned fibers of low density. Blood endothelial cells seeded on the fibers exhibited similar behavior as the LECs. Fiber alignment was preserved upon rolling the 2-dimensional substrate into the tubular geometry of a lymphatic vessel. The data suggest that aligned electrospun fibers may promote endothelial migration across the conduit in a manner that is independent of lymphatic growth factors.</p>
</abstract>
<kwd-group><kwd>lymphatic regeneration</kwd>
<kwd>endothelial cell</kwd>
<kwd>aligned fibers</kwd>
<kwd>biodegradable materials</kwd>
</kwd-group>
<contract-num rid="NS1">R21 NS062392-02
||NS</contract-num>
<contract-num rid="HL1">R21 HL093568-01A2
||HL</contract-num>
<contract-num rid="HL1">R15 HL093705-01
||HL</contract-num>
<contract-sponsor id="NS1">National Institute of Neurological Disorders and Stroke : NINDS</contract-sponsor>
<contract-sponsor id="HL1">National Heart, Lung, and Blood Institute : NHLBI</contract-sponsor>
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