Biomaterial guides for lymphatic endothelial cell alignment and migration.
Identifieur interne : 002862 ( PubMed/Corpus ); précédent : 002861; suivant : 002863Biomaterial guides for lymphatic endothelial cell alignment and migration.
Auteurs : Echoe M. Bouta ; Connor W. Mccarthy ; Alexander Keim ; Han Bing Wang ; Ryan J. Gilbert ; Jeremy GoldmanSource :
- Acta biomaterialia [ 1878-7568 ] ; 2011.
English descriptors
- KwdEn :
- MESH :
- chemical : Biocompatible Materials.
- cytology : Endothelium, Lymphatic Vessels.
- ultrastructure : Lymphatic Vessels.
- Cell Movement, Humans, Microscopy, Electron, Scanning.
Abstract
Axillary dissection during breast cancer surgery produces extensive lymphatic vessel damage that often leads to lifelong secondary lymphedema of the arm. We have developed a biodegradable material conduit for lymphatic vessel reconstruction where fibers electrospun along the conduit lumen promote endothelial cell alignment and migration in vitro. The diameter and density of the electrospun fibers were optimized for cell migration and direction on two-dimensional substrates by seeding human lymphatic endothelial cells (LECs) onto aligned fibers of varying diameters and densities, randomly oriented fibers, and film substrates with no fibers. We found that LECs became aligned in the fiber direction, with cells seeded on the randomly oriented fibers becoming oriented in random directions, whereas cells seeded on the highly aligned fibers became highly aligned. Cell migration was dependent upon fiber alignment and density, with optimal migration found on 1300 nm diameter aligned fibers of low density. Blood endothelial cells seeded on the fibers exhibited similar behavior as the LECs. Fiber alignment was preserved upon rolling the two-dimensional substrate into the tubular geometry of a lymphatic vessel. The data suggest that aligned electrospun fibers may promote endothelial migration across the conduit in a manner that is independent of lymphatic growth factors.
DOI: 10.1016/j.actbio.2010.10.016
PubMed: 20974299
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pubmed:20974299Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biomaterial guides for lymphatic endothelial cell alignment and migration.</title><author><name sortKey="Bouta, Echoe M" sort="Bouta, Echoe M" uniqKey="Bouta E" first="Echoe M" last="Bouta">Echoe M. Bouta</name><affiliation><nlm:affiliation>Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931-1295, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Mccarthy, Connor W" sort="Mccarthy, Connor W" uniqKey="Mccarthy C" first="Connor W" last="Mccarthy">Connor W. Mccarthy</name></author><author><name sortKey="Keim, Alexander" sort="Keim, Alexander" uniqKey="Keim A" first="Alexander" last="Keim">Alexander Keim</name></author><author><name sortKey="Wang, Han Bing" sort="Wang, Han Bing" uniqKey="Wang H" first="Han Bing" last="Wang">Han Bing Wang</name></author><author><name sortKey="Gilbert, Ryan J" sort="Gilbert, Ryan J" uniqKey="Gilbert R" first="Ryan J" last="Gilbert">Ryan J. Gilbert</name></author><author><name sortKey="Goldman, Jeremy" sort="Goldman, Jeremy" uniqKey="Goldman J" first="Jeremy" last="Goldman">Jeremy Goldman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:20974299</idno><idno type="pmid">20974299</idno><idno type="doi">10.1016/j.actbio.2010.10.016</idno><idno type="wicri:Area/PubMed/Corpus">002862</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002862</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Biomaterial guides for lymphatic endothelial cell alignment and migration.</title><author><name sortKey="Bouta, Echoe M" sort="Bouta, Echoe M" uniqKey="Bouta E" first="Echoe M" last="Bouta">Echoe M. Bouta</name><affiliation><nlm:affiliation>Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931-1295, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Mccarthy, Connor W" sort="Mccarthy, Connor W" uniqKey="Mccarthy C" first="Connor W" last="Mccarthy">Connor W. Mccarthy</name></author><author><name sortKey="Keim, Alexander" sort="Keim, Alexander" uniqKey="Keim A" first="Alexander" last="Keim">Alexander Keim</name></author><author><name sortKey="Wang, Han Bing" sort="Wang, Han Bing" uniqKey="Wang H" first="Han Bing" last="Wang">Han Bing Wang</name></author><author><name sortKey="Gilbert, Ryan J" sort="Gilbert, Ryan J" uniqKey="Gilbert R" first="Ryan J" last="Gilbert">Ryan J. Gilbert</name></author><author><name sortKey="Goldman, Jeremy" sort="Goldman, Jeremy" uniqKey="Goldman J" first="Jeremy" last="Goldman">Jeremy Goldman</name></author></analytic><series><title level="j">Acta biomaterialia</title><idno type="eISSN">1878-7568</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biocompatible Materials</term><term>Cell Movement</term><term>Endothelium (cytology)</term><term>Humans</term><term>Lymphatic Vessels (cytology)</term><term>Lymphatic Vessels (ultrastructure)</term><term>Microscopy, Electron, Scanning</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biocompatible Materials</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Endothelium</term><term>Lymphatic Vessels</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Lymphatic Vessels</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Movement</term><term>Humans</term><term>Microscopy, Electron, Scanning</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Axillary dissection during breast cancer surgery produces extensive lymphatic vessel damage that often leads to lifelong secondary lymphedema of the arm. We have developed a biodegradable material conduit for lymphatic vessel reconstruction where fibers electrospun along the conduit lumen promote endothelial cell alignment and migration in vitro. The diameter and density of the electrospun fibers were optimized for cell migration and direction on two-dimensional substrates by seeding human lymphatic endothelial cells (LECs) onto aligned fibers of varying diameters and densities, randomly oriented fibers, and film substrates with no fibers. We found that LECs became aligned in the fiber direction, with cells seeded on the randomly oriented fibers becoming oriented in random directions, whereas cells seeded on the highly aligned fibers became highly aligned. Cell migration was dependent upon fiber alignment and density, with optimal migration found on 1300 nm diameter aligned fibers of low density. Blood endothelial cells seeded on the fibers exhibited similar behavior as the LECs. Fiber alignment was preserved upon rolling the two-dimensional substrate into the tubular geometry of a lymphatic vessel. The data suggest that aligned electrospun fibers may promote endothelial migration across the conduit in a manner that is independent of lymphatic growth factors.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20974299</PMID><DateCreated><Year>2011</Year><Month>01</Month><Day>31</Day></DateCreated><DateCompleted><Year>2011</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2017</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1878-7568</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>3</Issue><PubDate><Year>2011</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Acta biomaterialia</Title><ISOAbbreviation>Acta Biomater</ISOAbbreviation></Journal><ArticleTitle>Biomaterial guides for lymphatic endothelial cell alignment and migration.</ArticleTitle><Pagination><MedlinePgn>1104-13</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.actbio.2010.10.016</ELocationID><Abstract><AbstractText>Axillary dissection during breast cancer surgery produces extensive lymphatic vessel damage that often leads to lifelong secondary lymphedema of the arm. We have developed a biodegradable material conduit for lymphatic vessel reconstruction where fibers electrospun along the conduit lumen promote endothelial cell alignment and migration in vitro. The diameter and density of the electrospun fibers were optimized for cell migration and direction on two-dimensional substrates by seeding human lymphatic endothelial cells (LECs) onto aligned fibers of varying diameters and densities, randomly oriented fibers, and film substrates with no fibers. We found that LECs became aligned in the fiber direction, with cells seeded on the randomly oriented fibers becoming oriented in random directions, whereas cells seeded on the highly aligned fibers became highly aligned. Cell migration was dependent upon fiber alignment and density, with optimal migration found on 1300 nm diameter aligned fibers of low density. Blood endothelial cells seeded on the fibers exhibited similar behavior as the LECs. Fiber alignment was preserved upon rolling the two-dimensional substrate into the tubular geometry of a lymphatic vessel. The data suggest that aligned electrospun fibers may promote endothelial migration across the conduit in a manner that is independent of lymphatic growth factors.</AbstractText><CopyrightInformation>Copyright © 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bouta</LastName><ForeName>Echoe M</ForeName><Initials>EM</Initials><AffiliationInfo><Affiliation>Department of Biomedical Engineering, Michigan Technological University, Houghton, MI 49931-1295, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McCarthy</LastName><ForeName>Connor W</ForeName><Initials>CW</Initials></Author><Author ValidYN="Y"><LastName>Keim</LastName><ForeName>Alexander</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Han Bing</ForeName><Initials>HB</Initials></Author><Author ValidYN="Y"><LastName>Gilbert</LastName><ForeName>Ryan J</ForeName><Initials>RJ</Initials></Author><Author ValidYN="Y"><LastName>Goldman</LastName><ForeName>Jeremy</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R15 HL093705</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R21 HL093568-01A2</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R21-HL-093568</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R21 NS062392</GrantID><Acronym>NS</Acronym><Agency>NINDS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R15 HL093705-01</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R15-HL-093705</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R21 HL093568</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R21 NS062392-02</GrantID><Acronym>NS</Acronym><Agency>NINDS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>10</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Acta Biomater</MedlineTA><NlmUniqueID>101233144</NlmUniqueID><ISSNLinking>1742-7061</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001672">Biocompatible Materials</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>J Biomed Mater Res A. 2009 Jul;90(1):186-95</RefSource><PMID Version="1">18491392</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Blood. 2010 May 13;115(19):3997-4005</RefSource><PMID 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