Microbiomes of Unreactive and Pathologically Altered Ileocecal Lymph Nodes of Slaughter Pigs
Identifieur interne : 002827 ( Main/Exploration ); précédent : 002826; suivant : 002828Microbiomes of Unreactive and Pathologically Altered Ileocecal Lymph Nodes of Slaughter Pigs
Auteurs : Evelyne Mann [Autriche] ; Monika Dzieciol [Autriche] ; Barbara U. Metzler-Zebeli [Autriche] ; Martin Wagner [Autriche] ; Stephan Schmitz-Esser [Autriche]Source :
- Applied and Environmental Microbiology [ 0099-2240 ] ; 2014.
Abstract
Microbe-laden dendritic cells are shifted to ileocecal lymph nodes (ICLNs), where microbes are concentrated and an adequate immune response is triggered. Hence, ICLNs are at a crucial position in immune anatomy and control processes of the local immune system. Pathological alterations in ICLNs, such as reactive hyperplasia,
Url:
DOI: 10.1128/AEM.03089-13
PubMed: 24141125
PubMed Central: 3911030
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>Microbe-laden dendritic cells are shifted to ileocecal lymph nodes (ICLNs), where microbes are concentrated and an adequate immune response is triggered. Hence, ICLNs are at a crucial position in immune anatomy and control processes of the local immune system. Pathological alterations in ICLNs, such as reactive hyperplasia, <named-content content-type="genus-species">lymphadenitis purulenta</named-content>
, or <named-content content-type="genus-species">granulomatosa</named-content>
, can harbor a multitude of pathogens and commensals, posing a potential zoonotic risk in animal production. The aim of this study was to characterize the microbial diversity of unreactive ICLNs of slaughter pigs and to investigate community shifts in reactive ICLNs altered by enlargement, purulence, or granulomatous formations. Pyrosequencing of 16S rRNA gene amplicons from 32 ICLNs yielded 175,313 sequences, clustering into 650 operational taxonomic units (OTUs). OTUs were assigned to 239 genera and 11 phyla. Besides a highly diverse bacterial community in ICLNs, we observed significant shifts in pathologically altered ICLNs. The relative abundances of <named-content content-type="genus-species">Cloacibacterium</named-content>
- and <named-content content-type="genus-species">Novosphingobium</named-content>
-associated OTUs and the genus <named-content content-type="genus-species">Faecalibacterium</named-content>
were significantly higher in unreactive ICLNs than in pathologically altered ICLNs. Enlarged ICLNs harbored significantly more <named-content content-type="genus-species">Lactobacillus</named-content>
- and <named-content content-type="genus-species">Clostridium</named-content>
-associated sequences. Relative abundances of <named-content content-type="genus-species">Mycoplasma</named-content>
, <named-content content-type="genus-species">Bacteroides</named-content>
, <named-content content-type="genus-species">Veillonella</named-content>
, and <named-content content-type="genus-species">Variovorax</named-content>
OTUs were significantly increased in granulomatous ICLNs, whereas abundances of <named-content content-type="genus-species">Pseudomonas</named-content>
, <named-content content-type="genus-species">Escherichia</named-content>
, and <named-content content-type="genus-species">Acinetobacter</named-content>
OTUs were significantly increased in purulent ICLNs (<italic>P</italic>
< 0.05). Correlation-based networks revealed interactions among OTUs in all ICLN groups, and discriminant analyses depicted discrimination in response to pathological alterations. This study is the first community-based survey in ICLNs of livestock animals and will provide a basis to broaden the knowledge of microbe-host interactions in pigs.</p>
</div>
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<affiliations><list><country><li>Autriche</li>
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<name sortKey="Mann, Evelyne" sort="Mann, Evelyne" uniqKey="Mann E" first="Evelyne" last="Mann">Evelyne Mann</name>
<name sortKey="Metzler Zebeli, Barbara U" sort="Metzler Zebeli, Barbara U" uniqKey="Metzler Zebeli B" first="Barbara U." last="Metzler-Zebeli">Barbara U. Metzler-Zebeli</name>
<name sortKey="Metzler Zebeli, Barbara U" sort="Metzler Zebeli, Barbara U" uniqKey="Metzler Zebeli B" first="Barbara U." last="Metzler-Zebeli">Barbara U. Metzler-Zebeli</name>
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<name sortKey="Schmitz Esser, Stephan" sort="Schmitz Esser, Stephan" uniqKey="Schmitz Esser S" first="Stephan" last="Schmitz-Esser">Stephan Schmitz-Esser</name>
<name sortKey="Wagner, Martin" sort="Wagner, Martin" uniqKey="Wagner M" first="Martin" last="Wagner">Martin Wagner</name>
<name sortKey="Wagner, Martin" sort="Wagner, Martin" uniqKey="Wagner M" first="Martin" last="Wagner">Martin Wagner</name>
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