Method for the quantitative measurement of collecting lymphatic vessel contraction in mice
Identifieur interne : 002828 ( Main/Exploration ); précédent : 002827; suivant : 002829Method for the quantitative measurement of collecting lymphatic vessel contraction in mice
Auteurs : Shan Liao ; Dennis Jones ; Gang Cheng ; Timothy P. PaderaSource :
- Journal of biological methods [ 2326-9901 ] ; 2014.
Abstract
Collecting lymphatic vessels are critical for the transport of lymph and its cellular contents to lymph nodes for both immune surveillance and the maintenance of tissue-fluid balance. Collecting lymphatic vessels drive lymph flow by autonomous contraction of smooth muscle cells that cover these vessels. Here we describe methods using intravital microscopy to image and quantify collecting lymphatic vessel contraction in mice. Our methods allow for the measurement of the strength of lymphatic contraction of an individual lymphangion in a mouse, which has not yet been demonstrated using other published methods. The ability to study murine collecting lymphatic vessel contraction—using the methods described here or other recently published techniques—allows the field to dissect the molecular mechanisms controlling lymphatic pumping under normal and pathological conditions using the wide variety of molecular tools and genetic models available in the mouse. We have used our methods to study lymphatic contraction in physiological and inflammatory conditions. The methods described here will facilitate the further study of lymphatic function in other pathological conditions that feature lymphatic complications.
Url:
DOI: 10.14440/jbm.2014.26
PubMed: 25512945
PubMed Central: 4264607
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 003623
- to stream Pmc, to step Curation: 003622
- to stream Pmc, to step Checkpoint: 001996
- to stream Ncbi, to step Merge: 006E60
- to stream Ncbi, to step Curation: 006E60
- to stream Ncbi, to step Checkpoint: 006E60
- to stream Main, to step Merge: 002832
- to stream Main, to step Curation: 002828
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Method for the quantitative measurement of collecting lymphatic vessel contraction in mice</title><author><name sortKey="Liao, Shan" sort="Liao, Shan" uniqKey="Liao S" first="Shan" last="Liao">Shan Liao</name></author><author><name sortKey="Jones, Dennis" sort="Jones, Dennis" uniqKey="Jones D" first="Dennis" last="Jones">Dennis Jones</name></author><author><name sortKey="Cheng, Gang" sort="Cheng, Gang" uniqKey="Cheng G" first="Gang" last="Cheng">Gang Cheng</name></author><author><name sortKey="Padera, Timothy P" sort="Padera, Timothy P" uniqKey="Padera T" first="Timothy P." last="Padera">Timothy P. Padera</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25512945</idno><idno type="pmc">4264607</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264607</idno><idno type="RBID">PMC:4264607</idno><idno type="doi">10.14440/jbm.2014.26</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">003623</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003623</idno><idno type="wicri:Area/Pmc/Curation">003622</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003622</idno><idno type="wicri:Area/Pmc/Checkpoint">001996</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001996</idno><idno type="wicri:Area/Ncbi/Merge">006E60</idno><idno type="wicri:Area/Ncbi/Curation">006E60</idno><idno type="wicri:Area/Ncbi/Checkpoint">006E60</idno><idno type="wicri:Area/Main/Merge">002832</idno><idno type="wicri:Area/Main/Curation">002828</idno><idno type="wicri:Area/Main/Exploration">002828</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Method for the quantitative measurement of collecting lymphatic vessel contraction in mice</title><author><name sortKey="Liao, Shan" sort="Liao, Shan" uniqKey="Liao S" first="Shan" last="Liao">Shan Liao</name></author><author><name sortKey="Jones, Dennis" sort="Jones, Dennis" uniqKey="Jones D" first="Dennis" last="Jones">Dennis Jones</name></author><author><name sortKey="Cheng, Gang" sort="Cheng, Gang" uniqKey="Cheng G" first="Gang" last="Cheng">Gang Cheng</name></author><author><name sortKey="Padera, Timothy P" sort="Padera, Timothy P" uniqKey="Padera T" first="Timothy P." last="Padera">Timothy P. Padera</name></author></analytic><series><title level="j">Journal of biological methods</title><idno type="eISSN">2326-9901</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P7">Collecting lymphatic vessels are critical for the transport of lymph and its cellular contents to lymph nodes for both immune surveillance and the maintenance of tissue-fluid balance. Collecting lymphatic vessels drive lymph flow by autonomous contraction of smooth muscle cells that cover these vessels. Here we describe methods using intravital microscopy to image and quantify collecting lymphatic vessel contraction in mice. Our methods allow for the measurement of the strength of lymphatic contraction of an individual lymphangion in a mouse, which has not yet been demonstrated using other published methods. The ability to study murine collecting lymphatic vessel contraction—using the methods described here or other recently published techniques—allows the field to dissect the molecular mechanisms controlling lymphatic pumping under normal and pathological conditions using the wide variety of molecular tools and genetic models available in the mouse. We have used our methods to study lymphatic contraction in physiological and inflammatory conditions. The methods described here will facilitate the further study of lymphatic function in other pathological conditions that feature lymphatic complications.</p></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Cheng, Gang" sort="Cheng, Gang" uniqKey="Cheng G" first="Gang" last="Cheng">Gang Cheng</name><name sortKey="Jones, Dennis" sort="Jones, Dennis" uniqKey="Jones D" first="Dennis" last="Jones">Dennis Jones</name><name sortKey="Liao, Shan" sort="Liao, Shan" uniqKey="Liao S" first="Shan" last="Liao">Shan Liao</name><name sortKey="Padera, Timothy P" sort="Padera, Timothy P" uniqKey="Padera T" first="Timothy P." last="Padera">Timothy P. Padera</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002828 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002828 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:4264607
|texte= Method for the quantitative measurement of collecting lymphatic vessel contraction in mice
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:25512945" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |