LymphedemaV1, Main, Exploration, bibRecord, 00DA43

Comparison of Dot-ELISA with Sandwich-ELISA for the detection of circulating antigens in patients with bancroftian filariasis.

Identifieur interne : 00DA43 ( Main/Exploration ); précédent : 00DA42; suivant : 00DA44

Comparison of Dot-ELISA with Sandwich-ELISA for the detection of circulating antigens in patients with bancroftian filariasis.

Auteurs : H J Zheng [République populaire de Chine] ; Z H Tao ; W F Cheng ; W F Piessens

Source :

The American journal of tropical medicine and hygiene [ 0002-9637 ] ; 1990.

RBID : pubmed:2196825

Descripteurs français

KwdFr :
- Animaux, Antigènes d'helminthe (sang), Filariose lymphatique (diagnostic), Filarioses (diagnostic), Humains, Test ELISA, Valeur prédictive des tests, Wuchereria (immunologie), Wuchereria bancrofti (immunologie).
MESH :
- diagnostic : Filariose lymphatique, Filarioses.
- immunologie : Wuchereria, Wuchereria bancrofti.
- sang : Antigènes d'helminthe.
- Animaux, Humains, Test ELISA, Valeur prédictive des tests.

English descriptors

KwdEn :
- Animals, Antigens, Helminth (blood), Elephantiasis, Filarial (diagnosis), Enzyme-Linked Immunosorbent Assay, Filariasis (diagnosis), Humans, Predictive Value of Tests, Wuchereria (immunology), Wuchereria bancrofti (immunology).
MESH :
- chemical , blood : Antigens, Helminth.
- diagnosis : Elephantiasis, Filarial, Filariasis.
- immunology : Wuchereria, Wuchereria bancrofti.
- Animals, Enzyme-Linked Immunosorbent Assay, Humans, Predictive Value of Tests.

Abstract

We compared the performance of a newly developed Dot-ELISA with that of a previously described Sandwich-ELISA to detect parasite antigens in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same sera were used in both tests. In the Dot-ELISA, 67 of 70 sera from microfilaremic donors were deemed to contain filarial antigens when screened at a dilution of 1:50. End titers were 1:80-1:1280. With the Sandwich-ELISA, 64 of the same sera were positive at a dilution of 1:10 and 42 were positive at a dilution of 1:50. End titers were 1:10-1:320. The specificity of both assays was greater than 95%, but their sensitivity was remarkably different. The Dot-ELISA could detect as little as 0.055 ng/ml microfilarial antigen added to normal human sera, whereas the lower limit with the Sandwich-ELISA was 10 ng/ml parasite antigen. Additionally, the Dot-ELISA does not require radioactivity or sophisticated equipment and, therefore, can be performed in virtually all filariasis-endemic areas.

PubMed: 2196825

Affiliations:

République populaire de Chine

Le document en format XML

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<term>Filariasis (diagnosis)</term>
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<term>Predictive Value of Tests</term>
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<term>Antigènes d'helminthe (sang)</term>
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<term>Filarioses (diagnostic)</term>
<term>Humains</term>
<term>Test ELISA</term>
<term>Valeur prédictive des tests</term>
<term>Wuchereria (immunologie)</term>
<term>Wuchereria bancrofti (immunologie)</term>
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<front><div type="abstract" xml:lang="en">We compared the performance of a newly developed Dot-ELISA with that of a previously described Sandwich-ELISA to detect parasite antigens in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same sera were used in both tests. In the Dot-ELISA, 67 of 70 sera from microfilaremic donors were deemed to contain filarial antigens when screened at a dilution of 1:50. End titers were 1:80-1:1280. With the Sandwich-ELISA, 64 of the same sera were positive at a dilution of 1:10 and 42 were positive at a dilution of 1:50. End titers were 1:10-1:320. The specificity of both assays was greater than 95%, but their sensitivity was remarkably different. The Dot-ELISA could detect as little as 0.055 ng/ml microfilarial antigen added to normal human sera, whereas the lower limit with the Sandwich-ELISA was 10 ng/ml parasite antigen. Additionally, the Dot-ELISA does not require radioactivity or sophisticated equipment and, therefore, can be performed in virtually all filariasis-endemic areas.</div>
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Serveur d'exploration sur le lymphœdème

Comparison of Dot-ELISA with Sandwich-ELISA for the detection of circulating antigens in patients with bancroftian filariasis.

Comparison of Dot-ELISA with Sandwich-ELISA for the detection of circulating antigens in patients with bancroftian filariasis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
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