Comparison of Dot-ELISA with Sandwich-ELISA for the detection of circulating antigens in patients with bancroftian filariasis.
Identifieur interne : 00DA43 ( Main/Curation ); précédent : 00DA42; suivant : 00DA44Comparison of Dot-ELISA with Sandwich-ELISA for the detection of circulating antigens in patients with bancroftian filariasis.
Auteurs : H J Zheng [République populaire de Chine] ; Z H Tao ; W F Cheng ; W F PiessensSource :
- The American journal of tropical medicine and hygiene [ 0002-9637 ] ; 1990.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Filariose lymphatique, Filarioses.
- immunologie : Wuchereria, Wuchereria bancrofti.
- sang : Antigènes d'helminthe.
- Animaux, Humains, Test ELISA, Valeur prédictive des tests.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antigens, Helminth.
- diagnosis : Elephantiasis, Filarial, Filariasis.
- immunology : Wuchereria, Wuchereria bancrofti.
- Animals, Enzyme-Linked Immunosorbent Assay, Humans, Predictive Value of Tests.
Abstract
We compared the performance of a newly developed Dot-ELISA with that of a previously described Sandwich-ELISA to detect parasite antigens in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same sera were used in both tests. In the Dot-ELISA, 67 of 70 sera from microfilaremic donors were deemed to contain filarial antigens when screened at a dilution of 1:50. End titers were 1:80-1:1280. With the Sandwich-ELISA, 64 of the same sera were positive at a dilution of 1:10 and 42 were positive at a dilution of 1:50. End titers were 1:10-1:320. The specificity of both assays was greater than 95%, but their sensitivity was remarkably different. The Dot-ELISA could detect as little as 0.055 ng/ml microfilarial antigen added to normal human sera, whereas the lower limit with the Sandwich-ELISA was 10 ng/ml parasite antigen. Additionally, the Dot-ELISA does not require radioactivity or sophisticated equipment and, therefore, can be performed in virtually all filariasis-endemic areas.
PubMed: 2196825
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :005D55
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :005D55
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :005D55
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :004710
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :004710
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :004710
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :00E643
Links to Exploration step
pubmed:2196825Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison of Dot-ELISA with Sandwich-ELISA for the detection of circulating antigens in patients with bancroftian filariasis.</title>
<author><name sortKey="Zheng, H J" sort="Zheng, H J" uniqKey="Zheng H" first="H J" last="Zheng">H J Zheng</name>
<affiliation wicri:level="1"><nlm:affiliation>Guizhou Provincial Institute of Parasitic Diseases, Guiyang, People's Republic of China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guizhou Provincial Institute of Parasitic Diseases, Guiyang</wicri:regionArea>
<wicri:noRegion>Guiyang</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tao, Z H" sort="Tao, Z H" uniqKey="Tao Z" first="Z H" last="Tao">Z H Tao</name>
</author>
<author><name sortKey="Cheng, W F" sort="Cheng, W F" uniqKey="Cheng W" first="W F" last="Cheng">W F Cheng</name>
</author>
<author><name sortKey="Piessens, W F" sort="Piessens, W F" uniqKey="Piessens W" first="W F" last="Piessens">W F Piessens</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1990">1990</date>
<idno type="RBID">pubmed:2196825</idno>
<idno type="pmid">2196825</idno>
<idno type="wicri:Area/PubMed/Corpus">005D55</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">005D55</idno>
<idno type="wicri:Area/PubMed/Curation">005D55</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">005D55</idno>
<idno type="wicri:Area/PubMed/Checkpoint">005D55</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">005D55</idno>
<idno type="wicri:Area/Ncbi/Merge">004710</idno>
<idno type="wicri:Area/Ncbi/Curation">004710</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">004710</idno>
<idno type="wicri:doubleKey">0002-9637:1990:Zheng H:comparison:of:dot</idno>
<idno type="wicri:Area/Main/Merge">00E643</idno>
<idno type="wicri:Area/Main/Curation">00DA43</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Comparison of Dot-ELISA with Sandwich-ELISA for the detection of circulating antigens in patients with bancroftian filariasis.</title>
<author><name sortKey="Zheng, H J" sort="Zheng, H J" uniqKey="Zheng H" first="H J" last="Zheng">H J Zheng</name>
<affiliation wicri:level="1"><nlm:affiliation>Guizhou Provincial Institute of Parasitic Diseases, Guiyang, People's Republic of China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guizhou Provincial Institute of Parasitic Diseases, Guiyang</wicri:regionArea>
<wicri:noRegion>Guiyang</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tao, Z H" sort="Tao, Z H" uniqKey="Tao Z" first="Z H" last="Tao">Z H Tao</name>
</author>
<author><name sortKey="Cheng, W F" sort="Cheng, W F" uniqKey="Cheng W" first="W F" last="Cheng">W F Cheng</name>
</author>
<author><name sortKey="Piessens, W F" sort="Piessens, W F" uniqKey="Piessens W" first="W F" last="Piessens">W F Piessens</name>
</author>
</analytic>
<series><title level="j">The American journal of tropical medicine and hygiene</title>
<idno type="ISSN">0002-9637</idno>
<imprint><date when="1990" type="published">1990</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antigens, Helminth (blood)</term>
<term>Elephantiasis, Filarial (diagnosis)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Filariasis (diagnosis)</term>
<term>Humans</term>
<term>Predictive Value of Tests</term>
<term>Wuchereria (immunology)</term>
<term>Wuchereria bancrofti (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Antigènes d'helminthe (sang)</term>
<term>Filariose lymphatique (diagnostic)</term>
<term>Filarioses (diagnostic)</term>
<term>Humains</term>
<term>Test ELISA</term>
<term>Valeur prédictive des tests</term>
<term>Wuchereria (immunologie)</term>
<term>Wuchereria bancrofti (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antigens, Helminth</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term>
<term>Filariasis</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term>
<term>Filarioses</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Wuchereria</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Wuchereria</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Antigènes d'helminthe</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Predictive Value of Tests</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Humains</term>
<term>Test ELISA</term>
<term>Valeur prédictive des tests</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We compared the performance of a newly developed Dot-ELISA with that of a previously described Sandwich-ELISA to detect parasite antigens in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same sera were used in both tests. In the Dot-ELISA, 67 of 70 sera from microfilaremic donors were deemed to contain filarial antigens when screened at a dilution of 1:50. End titers were 1:80-1:1280. With the Sandwich-ELISA, 64 of the same sera were positive at a dilution of 1:10 and 42 were positive at a dilution of 1:50. End titers were 1:10-1:320. The specificity of both assays was greater than 95%, but their sensitivity was remarkably different. The Dot-ELISA could detect as little as 0.055 ng/ml microfilarial antigen added to normal human sera, whereas the lower limit with the Sandwich-ELISA was 10 ng/ml parasite antigen. Additionally, the Dot-ELISA does not require radioactivity or sophisticated equipment and, therefore, can be performed in virtually all filariasis-endemic areas.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 00DA43 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 00DA43 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= LymphedemaV1 |flux= Main |étape= Curation |type= RBID |clé= pubmed:2196825 |texte= Comparison of Dot-ELISA with Sandwich-ELISA for the detection of circulating antigens in patients with bancroftian filariasis. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:2196825" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a LymphedemaV1
This area was generated with Dilib version V0.6.31. |