Comparison of Dot-ELISA with Sandwich-ELISA for the detection of circulating antigens in patients with bancroftian filariasis.
Identifieur interne : 005D55 ( PubMed/Curation ); précédent : 005D54; suivant : 005D56Comparison of Dot-ELISA with Sandwich-ELISA for the detection of circulating antigens in patients with bancroftian filariasis.
Auteurs : H J Zheng [République populaire de Chine] ; Z H Tao ; W F Cheng ; W F PiessensSource :
- The American journal of tropical medicine and hygiene [ 0002-9637 ] ; 1990.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Filariose lymphatique, Filarioses.
- immunologie : Wuchereria, Wuchereria bancrofti.
- sang : Antigènes d'helminthe.
- Animaux, Humains, Test ELISA, Valeur prédictive des tests.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antigens, Helminth.
- diagnosis : Elephantiasis, Filarial, Filariasis.
- immunology : Wuchereria, Wuchereria bancrofti.
- Animals, Enzyme-Linked Immunosorbent Assay, Humans, Predictive Value of Tests.
Abstract
We compared the performance of a newly developed Dot-ELISA with that of a previously described Sandwich-ELISA to detect parasite antigens in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same sera were used in both tests. In the Dot-ELISA, 67 of 70 sera from microfilaremic donors were deemed to contain filarial antigens when screened at a dilution of 1:50. End titers were 1:80-1:1280. With the Sandwich-ELISA, 64 of the same sera were positive at a dilution of 1:10 and 42 were positive at a dilution of 1:50. End titers were 1:10-1:320. The specificity of both assays was greater than 95%, but their sensitivity was remarkably different. The Dot-ELISA could detect as little as 0.055 ng/ml microfilarial antigen added to normal human sera, whereas the lower limit with the Sandwich-ELISA was 10 ng/ml parasite antigen. Additionally, the Dot-ELISA does not require radioactivity or sophisticated equipment and, therefore, can be performed in virtually all filariasis-endemic areas.
PubMed: 2196825
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pubmed:2196825Le document en format XML
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<author><name sortKey="Zheng, H J" sort="Zheng, H J" uniqKey="Zheng H" first="H J" last="Zheng">H J Zheng</name>
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<author><name sortKey="Cheng, W F" sort="Cheng, W F" uniqKey="Cheng W" first="W F" last="Cheng">W F Cheng</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antigens, Helminth (blood)</term>
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<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Filariasis (diagnosis)</term>
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<term>Predictive Value of Tests</term>
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<term>Filariose lymphatique (diagnostic)</term>
<term>Filarioses (diagnostic)</term>
<term>Humains</term>
<term>Test ELISA</term>
<term>Valeur prédictive des tests</term>
<term>Wuchereria (immunologie)</term>
<term>Wuchereria bancrofti (immunologie)</term>
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<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antigens, Helminth</term>
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<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term>
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<term>Filarioses</term>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Wuchereria</term>
<term>Wuchereria bancrofti</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Wuchereria</term>
<term>Wuchereria bancrofti</term>
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<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Antigènes d'helminthe</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
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<front><div type="abstract" xml:lang="en">We compared the performance of a newly developed Dot-ELISA with that of a previously described Sandwich-ELISA to detect parasite antigens in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same sera were used in both tests. In the Dot-ELISA, 67 of 70 sera from microfilaremic donors were deemed to contain filarial antigens when screened at a dilution of 1:50. End titers were 1:80-1:1280. With the Sandwich-ELISA, 64 of the same sera were positive at a dilution of 1:10 and 42 were positive at a dilution of 1:50. End titers were 1:10-1:320. The specificity of both assays was greater than 95%, but their sensitivity was remarkably different. The Dot-ELISA could detect as little as 0.055 ng/ml microfilarial antigen added to normal human sera, whereas the lower limit with the Sandwich-ELISA was 10 ng/ml parasite antigen. Additionally, the Dot-ELISA does not require radioactivity or sophisticated equipment and, therefore, can be performed in virtually all filariasis-endemic areas.</div>
</front>
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<Abstract><AbstractText>We compared the performance of a newly developed Dot-ELISA with that of a previously described Sandwich-ELISA to detect parasite antigens in sera from patients with bancroftian filariasis. The same monoclonal antibody and the same sera were used in both tests. In the Dot-ELISA, 67 of 70 sera from microfilaremic donors were deemed to contain filarial antigens when screened at a dilution of 1:50. End titers were 1:80-1:1280. With the Sandwich-ELISA, 64 of the same sera were positive at a dilution of 1:10 and 42 were positive at a dilution of 1:50. End titers were 1:10-1:320. The specificity of both assays was greater than 95%, but their sensitivity was remarkably different. The Dot-ELISA could detect as little as 0.055 ng/ml microfilarial antigen added to normal human sera, whereas the lower limit with the Sandwich-ELISA was 10 ng/ml parasite antigen. Additionally, the Dot-ELISA does not require radioactivity or sophisticated equipment and, therefore, can be performed in virtually all filariasis-endemic areas.</AbstractText>
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