Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis
Identifieur interne : 007E90 ( Main/Exploration ); précédent : 007E89; suivant : 007E91Screening differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis
Auteurs : Xiao-Nan Cui ; Jian-Wu Tang ; Li Hou ; Bo Song ; Li Li ; Ji-Wei LiuSource :
- World Journal of Gastroenterology : WJG [ 1007-9327 ] ; 2005.
Abstract
AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.
METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.
RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.
CONCLUSION: SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.
Url:
DOI: 10.3748/wjg.v11.i12.1837
PubMed: 15793877
PubMed Central: 4305887
Affiliations:
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Le document en format XML
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<author><name sortKey="Song, Bo" sort="Song, Bo" uniqKey="Song B" first="Bo" last="Song">Bo Song</name>
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<author><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name>
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<author><name sortKey="Liu, Ji Wei" sort="Liu, Ji Wei" uniqKey="Liu J" first="Ji-Wei" last="Liu">Ji-Wei Liu</name>
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<author><name sortKey="Tang, Jian Wu" sort="Tang, Jian Wu" uniqKey="Tang J" first="Jian-Wu" last="Tang">Jian-Wu Tang</name>
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<author><name sortKey="Hou, Li" sort="Hou, Li" uniqKey="Hou L" first="Li" last="Hou">Li Hou</name>
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<author><name sortKey="Song, Bo" sort="Song, Bo" uniqKey="Song B" first="Bo" last="Song">Bo Song</name>
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<author><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name>
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<front><div type="abstract" xml:lang="en"><p>AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.</p>
<p>METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.</p>
<p>RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.</p>
<p>CONCLUSION: SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.</p>
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<name sortKey="Hou, Li" sort="Hou, Li" uniqKey="Hou L" first="Li" last="Hou">Li Hou</name>
<name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name>
<name sortKey="Liu, Ji Wei" sort="Liu, Ji Wei" uniqKey="Liu J" first="Ji-Wei" last="Liu">Ji-Wei Liu</name>
<name sortKey="Song, Bo" sort="Song, Bo" uniqKey="Song B" first="Bo" last="Song">Bo Song</name>
<name sortKey="Tang, Jian Wu" sort="Tang, Jian Wu" uniqKey="Tang J" first="Jian-Wu" last="Tang">Jian-Wu Tang</name>
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