LymphedemaV1, Main, Exploration, bibRecord, 002919

Lymph Node Transplantation Results in Spontaneous Lymphatic Reconnection and Restoration of Lymphatic Flow

Identifieur interne : 002919 ( Main/Exploration ); précédent : 002918; suivant : 002920

Lymph Node Transplantation Results in Spontaneous Lymphatic Reconnection and Restoration of Lymphatic Flow

Auteurs : Seth Z. Aschen [États-Unis] ; Gina Farias-Eisner [États-Unis] ; Daniel A. Cuzzone [États-Unis] ; Nicholas J. Albano [États-Unis] ; Swapna Ghanta [États-Unis] ; Evan S. Weitman [États-Unis] ; Sagrario Ortega ; Babak J. Mehrara [États-Unis]

Source :

Plastic and reconstructive surgery [ 0032-1052 ] ; 2014.

RBID : PMC:4066306

Descripteurs français

KwdFr :
- Animaux, Mâle, Noeuds lymphatiques (physiologie), Noeuds lymphatiques (transplantation), Souris, Souris de lignée C57BL, Vaisseaux lymphatiques (), Vaisseaux lymphatiques (physiologie).
MESH :
- physiologie : Noeuds lymphatiques, Vaisseaux lymphatiques.
- Animaux, Mâle, Noeuds lymphatiques, Souris, Souris de lignée C57BL, Vaisseaux lymphatiques.

English descriptors

KwdEn :
- Animals, Lymph Nodes (physiology), Lymph Nodes (transplantation), Lymphatic Vessels (physiology), Lymphatic Vessels (surgery), Male, Mice, Mice, Inbred C57BL.
MESH :
- physiology : Lymph Nodes, Lymphatic Vessels.
- surgery : Lymphatic Vessels.
- transplantation : Lymph Nodes.
- Animals, Male, Mice, Mice, Inbred C57BL.

Abstract

Background

Although lymph node transplantation has been shown to improve lymphatic function the mechanisms regulating lymphatic vessel reconnection and functional status of lymph nodes remains poorly understood.

Methods

We developed and used LacZ lymphatic reporter mice to examine the lineage of lymphatic vessels infiltrating transferred lymph nodes. In addition, we analyzed lymphatic function, expression of vascular endothelial growth factor (VEGF-C), maintenance of T and B cell zone, and anatomic localization of lymphatics and high endothelial venules (HEVs).

Results

Reporter mice were specific and highly sensitive in identifying lymphatic vessels. Lymph node transfer was associated with rapid return of lymphatic function and clearance of Tc⁹⁹ secondary to a massive infiltration of recipient mouse lymphatics and putative connections to donor lymphatics. T and B cell populations in the lymph node were maintained. These changes correlated with marked increases in the expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Newly formed lymphatic channels in transferred lymph nodes were in close anatomic proximity to HEVs.

Conclusions

Transferred lymph nodes have rapid infiltration of functional host lymphatic vessels and maintain T and B cell populations. This process correlates with increased endogenous expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Anatomic proximity of newly formed lymphatics and HEVs supports the hypothesis that lymph node transfer can improve lymphedema by exchanges with the systemic circulation.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066306

DOI: 10.1097/01.prs.0000436840.69752.7e
PubMed: 24469165
PubMed Central: 4066306

Affiliations:

Le document en format XML

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<author><name sortKey="Mehrara, Babak J" sort="Mehrara, Babak J" uniqKey="Mehrara B" first="Babak J." last="Mehrara">Babak J. Mehrara</name>
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<country xml:lang="fr">États-Unis</country>
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<series><title level="j">Plastic and reconstructive surgery</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Lymph Nodes (physiology)</term>
<term>Lymph Nodes (transplantation)</term>
<term>Lymphatic Vessels (physiology)</term>
<term>Lymphatic Vessels (surgery)</term>
<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Mâle</term>
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<term>Noeuds lymphatiques (transplantation)</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
<term>Vaisseaux lymphatiques ()</term>
<term>Vaisseaux lymphatiques (physiologie)</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Noeuds lymphatiques</term>
<term>Vaisseaux lymphatiques</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Lymph Nodes</term>
<term>Lymphatic Vessels</term>
</keywords>
<keywords scheme="MESH" qualifier="surgery" xml:lang="en"><term>Lymphatic Vessels</term>
</keywords>
<keywords scheme="MESH" qualifier="transplantation" xml:lang="en"><term>Lymph Nodes</term>
</keywords>
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<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Mâle</term>
<term>Noeuds lymphatiques</term>
<term>Souris</term>
<term>Souris de lignée C57BL</term>
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<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Background</title>
<p id="P1">Although lymph node transplantation has been shown to improve lymphatic function the mechanisms regulating lymphatic vessel reconnection and functional status of lymph nodes remains poorly understood.</p>
</sec>
<sec id="S2"><title>Methods</title>
<p id="P2">We developed and used LacZ lymphatic reporter mice to examine the lineage of lymphatic vessels infiltrating transferred lymph nodes. In addition, we analyzed lymphatic function, expression of vascular endothelial growth factor (VEGF-C), maintenance of T and B cell zone, and anatomic localization of lymphatics and high endothelial venules (HEVs).</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Reporter mice were specific and highly sensitive in identifying lymphatic vessels. Lymph node transfer was associated with rapid return of lymphatic function and clearance of Tc<sup>99</sup>
 secondary to a massive infiltration of recipient mouse lymphatics and putative connections to donor lymphatics. T and B cell populations in the lymph node were maintained. These changes correlated with marked increases in the expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Newly formed lymphatic channels in transferred lymph nodes were in close anatomic proximity to HEVs.</p>
</sec>
<sec id="S4"><title>Conclusions</title>
<p id="P4">Transferred lymph nodes have rapid infiltration of functional host lymphatic vessels and maintain T and B cell populations. This process correlates with increased endogenous expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Anatomic proximity of newly formed lymphatics and HEVs supports the hypothesis that lymph node transfer can improve lymphedema by exchanges with the systemic circulation.</p>
</sec>
</div>
</front>
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<country name="États-Unis"><region name="État de New York"><name sortKey="Aschen, Seth Z" sort="Aschen, Seth Z" uniqKey="Aschen S" first="Seth Z." last="Aschen">Seth Z. Aschen</name>
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<name sortKey="Albano, Nicholas J" sort="Albano, Nicholas J" uniqKey="Albano N" first="Nicholas J." last="Albano">Nicholas J. Albano</name>
<name sortKey="Cuzzone, Daniel A" sort="Cuzzone, Daniel A" uniqKey="Cuzzone D" first="Daniel A." last="Cuzzone">Daniel A. Cuzzone</name>
<name sortKey="Farias Eisner, Gina" sort="Farias Eisner, Gina" uniqKey="Farias Eisner G" first="Gina" last="Farias-Eisner">Gina Farias-Eisner</name>
<name sortKey="Ghanta, Swapna" sort="Ghanta, Swapna" uniqKey="Ghanta S" first="Swapna" last="Ghanta">Swapna Ghanta</name>
<name sortKey="Mehrara, Babak J" sort="Mehrara, Babak J" uniqKey="Mehrara B" first="Babak J." last="Mehrara">Babak J. Mehrara</name>
<name sortKey="Weitman, Evan S" sort="Weitman, Evan S" uniqKey="Weitman E" first="Evan S." last="Weitman">Evan S. Weitman</name>
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Serveur d'exploration sur le lymphœdème

Lymph Node Transplantation Results in Spontaneous Lymphatic Reconnection and Restoration of Lymphatic Flow

Lymph Node Transplantation Results in Spontaneous Lymphatic Reconnection and Restoration of Lymphatic Flow

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
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