Lymph Node Transplantation Results in Spontaneous Lymphatic Reconnection and Restoration of Lymphatic Flow
Identifieur interne : 003644 ( Pmc/Corpus ); précédent : 003643; suivant : 003645Lymph Node Transplantation Results in Spontaneous Lymphatic Reconnection and Restoration of Lymphatic Flow
Auteurs : Seth Z. Aschen ; Gina Farias-Eisner ; Daniel A. Cuzzone ; Nicholas J. Albano ; Swapna Ghanta ; Evan S. Weitman ; Sagrario Ortega ; Babak J. MehraraSource :
- Plastic and reconstructive surgery [ 0032-1052 ] ; 2014.
Abstract
Although lymph node transplantation has been shown to improve lymphatic function the mechanisms regulating lymphatic vessel reconnection and functional status of lymph nodes remains poorly understood.
We developed and used LacZ lymphatic reporter mice to examine the lineage of lymphatic vessels infiltrating transferred lymph nodes. In addition, we analyzed lymphatic function, expression of vascular endothelial growth factor (VEGF-C), maintenance of T and B cell zone, and anatomic localization of lymphatics and high endothelial venules (HEVs).
Reporter mice were specific and highly sensitive in identifying lymphatic vessels. Lymph node transfer was associated with rapid return of lymphatic function and clearance of Tc99 secondary to a massive infiltration of recipient mouse lymphatics and putative connections to donor lymphatics. T and B cell populations in the lymph node were maintained. These changes correlated with marked increases in the expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Newly formed lymphatic channels in transferred lymph nodes were in close anatomic proximity to HEVs.
Transferred lymph nodes have rapid infiltration of functional host lymphatic vessels and maintain T and B cell populations. This process correlates with increased endogenous expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Anatomic proximity of newly formed lymphatics and HEVs supports the hypothesis that lymph node transfer can improve lymphedema by exchanges with the systemic circulation.
Url:
DOI: 10.1097/01.prs.0000436840.69752.7e
PubMed: 24469165
PubMed Central: 4066306
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PMC:4066306Le document en format XML
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<author><name sortKey="Aschen, Seth Z" sort="Aschen, Seth Z" uniqKey="Aschen S" first="Seth Z." last="Aschen">Seth Z. Aschen</name>
<affiliation><nlm:aff id="A1">The Division of Plastic and Reconstructive Surgery Memorial Sloan-Kettering Cancer Center New York, NY 10065</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Farias Eisner, Gina" sort="Farias Eisner, Gina" uniqKey="Farias Eisner G" first="Gina" last="Farias-Eisner">Gina Farias-Eisner</name>
<affiliation><nlm:aff id="A1">The Division of Plastic and Reconstructive Surgery Memorial Sloan-Kettering Cancer Center New York, NY 10065</nlm:aff>
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<author><name sortKey="Cuzzone, Daniel A" sort="Cuzzone, Daniel A" uniqKey="Cuzzone D" first="Daniel A." last="Cuzzone">Daniel A. Cuzzone</name>
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<author><name sortKey="Albano, Nicholas J" sort="Albano, Nicholas J" uniqKey="Albano N" first="Nicholas J." last="Albano">Nicholas J. Albano</name>
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<author><name sortKey="Ghanta, Swapna" sort="Ghanta, Swapna" uniqKey="Ghanta S" first="Swapna" last="Ghanta">Swapna Ghanta</name>
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<author><name sortKey="Weitman, Evan S" sort="Weitman, Evan S" uniqKey="Weitman E" first="Evan S." last="Weitman">Evan S. Weitman</name>
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<author><name sortKey="Ortega, Sagrario" sort="Ortega, Sagrario" uniqKey="Ortega S" first="Sagrario" last="Ortega">Sagrario Ortega</name>
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<author><name sortKey="Mehrara, Babak J" sort="Mehrara, Babak J" uniqKey="Mehrara B" first="Babak J." last="Mehrara">Babak J. Mehrara</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Lymph Node Transplantation Results in Spontaneous Lymphatic Reconnection and Restoration of Lymphatic Flow</title>
<author><name sortKey="Aschen, Seth Z" sort="Aschen, Seth Z" uniqKey="Aschen S" first="Seth Z." last="Aschen">Seth Z. Aschen</name>
<affiliation><nlm:aff id="A1">The Division of Plastic and Reconstructive Surgery Memorial Sloan-Kettering Cancer Center New York, NY 10065</nlm:aff>
</affiliation>
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<author><name sortKey="Farias Eisner, Gina" sort="Farias Eisner, Gina" uniqKey="Farias Eisner G" first="Gina" last="Farias-Eisner">Gina Farias-Eisner</name>
<affiliation><nlm:aff id="A1">The Division of Plastic and Reconstructive Surgery Memorial Sloan-Kettering Cancer Center New York, NY 10065</nlm:aff>
</affiliation>
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<author><name sortKey="Cuzzone, Daniel A" sort="Cuzzone, Daniel A" uniqKey="Cuzzone D" first="Daniel A." last="Cuzzone">Daniel A. Cuzzone</name>
<affiliation><nlm:aff id="A1">The Division of Plastic and Reconstructive Surgery Memorial Sloan-Kettering Cancer Center New York, NY 10065</nlm:aff>
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<author><name sortKey="Albano, Nicholas J" sort="Albano, Nicholas J" uniqKey="Albano N" first="Nicholas J." last="Albano">Nicholas J. Albano</name>
<affiliation><nlm:aff id="A1">The Division of Plastic and Reconstructive Surgery Memorial Sloan-Kettering Cancer Center New York, NY 10065</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ghanta, Swapna" sort="Ghanta, Swapna" uniqKey="Ghanta S" first="Swapna" last="Ghanta">Swapna Ghanta</name>
<affiliation><nlm:aff id="A1">The Division of Plastic and Reconstructive Surgery Memorial Sloan-Kettering Cancer Center New York, NY 10065</nlm:aff>
</affiliation>
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<author><name sortKey="Weitman, Evan S" sort="Weitman, Evan S" uniqKey="Weitman E" first="Evan S." last="Weitman">Evan S. Weitman</name>
<affiliation><nlm:aff id="A1">The Division of Plastic and Reconstructive Surgery Memorial Sloan-Kettering Cancer Center New York, NY 10065</nlm:aff>
</affiliation>
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<author><name sortKey="Ortega, Sagrario" sort="Ortega, Sagrario" uniqKey="Ortega S" first="Sagrario" last="Ortega">Sagrario Ortega</name>
<affiliation><nlm:aff id="A2"> Biotechnology Programme Spanish National Cancer Research Centre (CNIO) Madrid, Spain 28029</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mehrara, Babak J" sort="Mehrara, Babak J" uniqKey="Mehrara B" first="Babak J." last="Mehrara">Babak J. Mehrara</name>
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</affiliation>
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<series><title level="j">Plastic and reconstructive surgery</title>
<idno type="ISSN">0032-1052</idno>
<idno type="eISSN">1529-4242</idno>
<imprint><date when="2014">2014</date>
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<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Background</title>
<p id="P1">Although lymph node transplantation has been shown to improve lymphatic function the mechanisms regulating lymphatic vessel reconnection and functional status of lymph nodes remains poorly understood.</p>
</sec>
<sec id="S2"><title>Methods</title>
<p id="P2">We developed and used LacZ lymphatic reporter mice to examine the lineage of lymphatic vessels infiltrating transferred lymph nodes. In addition, we analyzed lymphatic function, expression of vascular endothelial growth factor (VEGF-C), maintenance of T and B cell zone, and anatomic localization of lymphatics and high endothelial venules (HEVs).</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Reporter mice were specific and highly sensitive in identifying lymphatic vessels. Lymph node transfer was associated with rapid return of lymphatic function and clearance of Tc<sup>99</sup>
secondary to a massive infiltration of recipient mouse lymphatics and putative connections to donor lymphatics. T and B cell populations in the lymph node were maintained. These changes correlated with marked increases in the expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Newly formed lymphatic channels in transferred lymph nodes were in close anatomic proximity to HEVs.</p>
</sec>
<sec id="S4"><title>Conclusions</title>
<p id="P4">Transferred lymph nodes have rapid infiltration of functional host lymphatic vessels and maintain T and B cell populations. This process correlates with increased endogenous expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Anatomic proximity of newly formed lymphatics and HEVs supports the hypothesis that lymph node transfer can improve lymphedema by exchanges with the systemic circulation.</p>
</sec>
</div>
</front>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">1306050</journal-id>
<journal-id journal-id-type="pubmed-jr-id">6482</journal-id>
<journal-id journal-id-type="nlm-ta">Plast Reconstr Surg</journal-id>
<journal-id journal-id-type="iso-abbrev">Plast. Reconstr. Surg.</journal-id>
<journal-title-group><journal-title>Plastic and reconstructive surgery</journal-title>
</journal-title-group>
<issn pub-type="ppub">0032-1052</issn>
<issn pub-type="epub">1529-4242</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24469165</article-id>
<article-id pub-id-type="pmc">4066306</article-id>
<article-id pub-id-type="doi">10.1097/01.prs.0000436840.69752.7e</article-id>
<article-id pub-id-type="manuscript">NIHMS542812</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Lymph Node Transplantation Results in Spontaneous Lymphatic Reconnection and Restoration of Lymphatic Flow</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Aschen</surname>
<given-names>Seth Z.</given-names>
</name>
<degrees>BS</degrees>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Farias-Eisner</surname>
<given-names>Gina</given-names>
</name>
<degrees>BA</degrees>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Cuzzone</surname>
<given-names>Daniel A.</given-names>
</name>
<degrees>MD</degrees>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Albano</surname>
<given-names>Nicholas J.</given-names>
</name>
<degrees>BS</degrees>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ghanta</surname>
<given-names>Swapna</given-names>
</name>
<degrees>MD</degrees>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Weitman</surname>
<given-names>Evan S.</given-names>
</name>
<degrees>MD</degrees>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ortega</surname>
<given-names>Sagrario</given-names>
</name>
<degrees>PhD</degrees>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mehrara</surname>
<given-names>Babak J.</given-names>
</name>
<degrees>MD</degrees>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
The Division of Plastic and Reconstructive Surgery Memorial Sloan-Kettering Cancer Center New York, NY 10065</aff>
<aff id="A2"><label>2</label>
Biotechnology Programme Spanish National Cancer Research Centre (CNIO) Madrid, Spain 28029</aff>
<author-notes><corresp id="CR1"><bold>Correspondence</bold>
: Babak J. Mehrara, MD 1275 York Avenue Suite MRI 1005 New York, NY 10065 212-639-8639 212-717-3677 Fax <email>mehrarab@mskcc.org</email>
</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>3</day>
<month>6</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="ppub"><month>2</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>01</day>
<month>2</month>
<year>2015</year>
</pub-date>
<volume>133</volume>
<issue>2</issue>
<fpage>301</fpage>
<lpage>310</lpage>
<pmc-comment>elocation-id from pubmed: 10.1097/01.prs.0000436840.69752.7e</pmc-comment>
<abstract><sec id="S1"><title>Background</title>
<p id="P1">Although lymph node transplantation has been shown to improve lymphatic function the mechanisms regulating lymphatic vessel reconnection and functional status of lymph nodes remains poorly understood.</p>
</sec>
<sec id="S2"><title>Methods</title>
<p id="P2">We developed and used LacZ lymphatic reporter mice to examine the lineage of lymphatic vessels infiltrating transferred lymph nodes. In addition, we analyzed lymphatic function, expression of vascular endothelial growth factor (VEGF-C), maintenance of T and B cell zone, and anatomic localization of lymphatics and high endothelial venules (HEVs).</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Reporter mice were specific and highly sensitive in identifying lymphatic vessels. Lymph node transfer was associated with rapid return of lymphatic function and clearance of Tc<sup>99</sup>
secondary to a massive infiltration of recipient mouse lymphatics and putative connections to donor lymphatics. T and B cell populations in the lymph node were maintained. These changes correlated with marked increases in the expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Newly formed lymphatic channels in transferred lymph nodes were in close anatomic proximity to HEVs.</p>
</sec>
<sec id="S4"><title>Conclusions</title>
<p id="P4">Transferred lymph nodes have rapid infiltration of functional host lymphatic vessels and maintain T and B cell populations. This process correlates with increased endogenous expression of VEGF-C in the perinodal fat and infiltrating lymphatics. Anatomic proximity of newly formed lymphatics and HEVs supports the hypothesis that lymph node transfer can improve lymphedema by exchanges with the systemic circulation.</p>
</sec>
</abstract>
<kwd-group><kwd>Lymph node</kwd>
<kwd>transfer</kwd>
<kwd>lymphatic regeneration</kwd>
<kwd>lymphatic flow</kwd>
<kwd>lymphedema</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
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