[The use of the immunoperoxidase test with monoclonal antibodies for detecting influenza A viruses].
Identifieur interne : 000405 ( PubMed/Corpus ); précédent : 000404; suivant : 000406[The use of the immunoperoxidase test with monoclonal antibodies for detecting influenza A viruses].
Auteurs : A A Bakova ; V A Gusev ; I A Popov ; A I Gordienko ; Iu L. Krivorutchenko ; Iu S. KrivosheinSource :
- Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993) [ 1028-0987 ]
English descriptors
- KwdEn :
- Allantois (immunology), Allantois (virology), Animals, Antibodies, Monoclonal, Antigens, Viral (analysis), Chick Embryo, Chorion (immunology), Chorion (virology), Hemagglutinins, Viral (immunology), Humans, Hybridomas (immunology), Immunoenzyme Techniques, Influenza A virus (immunology), Viral Matrix Proteins (immunology).
- MESH :
- chemical , analysis : Antigens, Viral.
- chemical , immunology : Hemagglutinins, Viral, Viral Matrix Proteins.
- chemical : Antibodies, Monoclonal.
- immunology : Allantois, Chorion, Hybridomas, Influenza A virus.
- virology : Allantois, Chorion.
- Animals, Chick Embryo, Humans, Immunoenzyme Techniques.
Abstract
The immunoperoxidase test was used to detect influenza virus in cells of a chorionallanthois shell of infected chicken embryos. Application of monoclonal antibodies D8 and A11 in the analysis has permitted detecting reproduction of type A (subtypes H1N1, H2N2, H3N2) viruses, the PGA titre of the respective allantois liquids being not lower than 1:16. The matrix protein and hemagglutinin, detection of which underlies this analysis, were found on the cell membrane, in the perinucleus region and as cytoplasmic inclusions.
PubMed: 8548071
Links to Exploration step
pubmed:8548071Le document en format XML
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<author><name sortKey="Bakova, A A" sort="Bakova, A A" uniqKey="Bakova A" first="A A" last="Bakova">A A Bakova</name>
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<author><name sortKey="Gusev, V A" sort="Gusev, V A" uniqKey="Gusev V" first="V A" last="Gusev">V A Gusev</name>
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<author><name sortKey="Popov, I A" sort="Popov, I A" uniqKey="Popov I" first="I A" last="Popov">I A Popov</name>
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<author><name sortKey="Gordienko, A I" sort="Gordienko, A I" uniqKey="Gordienko A" first="A I" last="Gordienko">A I Gordienko</name>
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<author><name sortKey="Krivorutchenko, Iu L" sort="Krivorutchenko, Iu L" uniqKey="Krivorutchenko I" first="Iu L" last="Krivorutchenko">Iu L. Krivorutchenko</name>
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<author><name sortKey="Krivoshein, Iu S" sort="Krivoshein, Iu S" uniqKey="Krivoshein I" first="Iu S" last="Krivoshein">Iu S. Krivoshein</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[The use of the immunoperoxidase test with monoclonal antibodies for detecting influenza A viruses].</title>
<author><name sortKey="Bakova, A A" sort="Bakova, A A" uniqKey="Bakova A" first="A A" last="Bakova">A A Bakova</name>
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<author><name sortKey="Gusev, V A" sort="Gusev, V A" uniqKey="Gusev V" first="V A" last="Gusev">V A Gusev</name>
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<author><name sortKey="Popov, I A" sort="Popov, I A" uniqKey="Popov I" first="I A" last="Popov">I A Popov</name>
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<author><name sortKey="Gordienko, A I" sort="Gordienko, A I" uniqKey="Gordienko A" first="A I" last="Gordienko">A I Gordienko</name>
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<author><name sortKey="Krivorutchenko, Iu L" sort="Krivorutchenko, Iu L" uniqKey="Krivorutchenko I" first="Iu L" last="Krivorutchenko">Iu L. Krivorutchenko</name>
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<series><title level="j">Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993)</title>
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<term>Allantois (virology)</term>
<term>Animals</term>
<term>Antibodies, Monoclonal</term>
<term>Antigens, Viral (analysis)</term>
<term>Chick Embryo</term>
<term>Chorion (immunology)</term>
<term>Chorion (virology)</term>
<term>Hemagglutinins, Viral (immunology)</term>
<term>Humans</term>
<term>Hybridomas (immunology)</term>
<term>Immunoenzyme Techniques</term>
<term>Influenza A virus (immunology)</term>
<term>Viral Matrix Proteins (immunology)</term>
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<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Hemagglutinins, Viral</term>
<term>Viral Matrix Proteins</term>
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<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Allantois</term>
<term>Chorion</term>
<term>Hybridomas</term>
<term>Influenza A virus</term>
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<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Allantois</term>
<term>Chorion</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chick Embryo</term>
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<term>Immunoenzyme Techniques</term>
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<front><div type="abstract" xml:lang="en">The immunoperoxidase test was used to detect influenza virus in cells of a chorionallanthois shell of infected chicken embryos. Application of monoclonal antibodies D8 and A11 in the analysis has permitted detecting reproduction of type A (subtypes H1N1, H2N2, H3N2) viruses, the PGA titre of the respective allantois liquids being not lower than 1:16. The matrix protein and hemagglutinin, detection of which underlies this analysis, were found on the cell membrane, in the perinucleus region and as cytoplasmic inclusions.</div>
</front>
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<DateCompleted><Year>1996</Year>
<Month>02</Month>
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<Article PubModel="Print"><Journal><ISSN IssnType="Print">1028-0987</ISSN>
<JournalIssue CitedMedium="Print"><Volume>57</Volume>
<Issue>4</Issue>
<PubDate><MedlineDate>1995 Jul-Aug</MedlineDate>
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<Title>Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993)</Title>
<ISOAbbreviation>Mikrobiol. Z.</ISOAbbreviation>
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<ArticleTitle>[The use of the immunoperoxidase test with monoclonal antibodies for detecting influenza A viruses].</ArticleTitle>
<Pagination><MedlinePgn>54-9</MedlinePgn>
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<Abstract><AbstractText>The immunoperoxidase test was used to detect influenza virus in cells of a chorionallanthois shell of infected chicken embryos. Application of monoclonal antibodies D8 and A11 in the analysis has permitted detecting reproduction of type A (subtypes H1N1, H2N2, H3N2) viruses, the PGA titre of the respective allantois liquids being not lower than 1:16. The matrix protein and hemagglutinin, detection of which underlies this analysis, were found on the cell membrane, in the perinucleus region and as cytoplasmic inclusions.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bakova</LastName>
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<Initials>AA</Initials>
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<Author ValidYN="Y"><LastName>Gusev</LastName>
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<Author ValidYN="Y"><LastName>Popov</LastName>
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<VernacularTitle>Ispol'zovanie immunoperoksidaznogo testa s monoklonal'nymi antitelami dlia vyiavleniia virusov grippa A.</VernacularTitle>
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<MedlineJournalInfo><Country>Ukraine</Country>
<MedlineTA>Mikrobiol Z</MedlineTA>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D000482" MajorTopicYN="N">Allantois</DescriptorName>
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<MeshHeading><DescriptorName UI="D002642" MajorTopicYN="N">Chick Embryo</DescriptorName>
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<MeshHeading><DescriptorName UI="D002823" MajorTopicYN="N">Chorion</DescriptorName>
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<MeshHeading><DescriptorName UI="D006825" MajorTopicYN="N">Hybridomas</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D007124" MajorTopicYN="Y">Immunoenzyme Techniques</DescriptorName>
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<MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
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<MeshHeading><DescriptorName UI="D014763" MajorTopicYN="N">Viral Matrix Proteins</DescriptorName>
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