Genetic stability of cold-adapted A/Leningrad/134/47/57 (H2N2) influenza virus: sequence analysis of live cold-adapted reassortant vaccine strains before and after replication in children.
Identifieur interne : 000406 ( PubMed/Corpus ); précédent : 000405; suivant : 000407Genetic stability of cold-adapted A/Leningrad/134/47/57 (H2N2) influenza virus: sequence analysis of live cold-adapted reassortant vaccine strains before and after replication in children.
Auteurs : A I Klimov ; A Y Egorov ; M I Gushchina ; T E Medvedeva ; W C Gamble ; L G Rudenko ; G I Alexandrova ; N J CoxSource :
- The Journal of general virology [ 0022-1317 ] ; 1995.
English descriptors
- KwdEn :
- Acclimatization, Amino Acid Sequence, Base Sequence, Child, Codon, Cold Temperature, Conserved Sequence, DNA Primers, Genome, Viral, Humans, Influenza A Virus, H2N2 Subtype, Influenza A virus (genetics), Influenza A virus (immunology), Influenza A virus (physiology), Influenza Vaccines, Molecular Sequence Data, Polymerase Chain Reaction, Russia, Vaccines, Attenuated, Virus Replication.
- MESH :
- chemical : Codon, DNA Primers, Influenza Vaccines, Vaccines, Attenuated.
- geographic : Russia.
- genetics : Influenza A virus.
- immunology : Influenza A virus.
- physiology : Influenza A virus.
- Acclimatization, Amino Acid Sequence, Base Sequence, Child, Cold Temperature, Conserved Sequence, Genome, Viral, Humans, Influenza A Virus, H2N2 Subtype, Molecular Sequence Data, Polymerase Chain Reaction, Virus Replication.
Abstract
We previously reported that the A/Leningrad/134/47/57 (H2N2) cold-adapted virus (A/Len/47) used in preparing reassortant live attenuated vaccines for children acquired 14 (11 coding) mutations in genes coding for proteins other than haemagglutinin and neuraminidase during cold-adaptation. Preservation of these mutations in genomes of viruses isolated from children on the second, fifth, or eighth day after vaccination was examined by sequence analysis. The sequence data demonstrated that all nine coding mutations selected for examination were conserved in the genomes of all 11 strains investigated, indicating that the mutations accompanying cold-adaptation and attenuation of the A/Len/47 master vaccine are highly stable.
DOI: 10.1099/0022-1317-76-6-1521
PubMed: 7782782
Links to Exploration step
pubmed:7782782Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genetic stability of cold-adapted A/Leningrad/134/47/57 (H2N2) influenza virus: sequence analysis of live cold-adapted reassortant vaccine strains before and after replication in children.</title><author><name sortKey="Klimov, A I" sort="Klimov, A I" uniqKey="Klimov A" first="A I" last="Klimov">A I Klimov</name><affiliation><nlm:affiliation>Influenza Branch G-16, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Egorov, A Y" sort="Egorov, A Y" uniqKey="Egorov A" first="A Y" last="Egorov">A Y Egorov</name></author><author><name sortKey="Gushchina, M I" sort="Gushchina, M I" uniqKey="Gushchina M" first="M I" last="Gushchina">M I Gushchina</name></author><author><name sortKey="Medvedeva, T E" sort="Medvedeva, T E" uniqKey="Medvedeva T" first="T E" last="Medvedeva">T E Medvedeva</name></author><author><name sortKey="Gamble, W C" sort="Gamble, W C" uniqKey="Gamble W" first="W C" last="Gamble">W C Gamble</name></author><author><name sortKey="Rudenko, L G" sort="Rudenko, L G" uniqKey="Rudenko L" first="L G" last="Rudenko">L G Rudenko</name></author><author><name sortKey="Alexandrova, G I" sort="Alexandrova, G I" uniqKey="Alexandrova G" first="G I" last="Alexandrova">G I Alexandrova</name></author><author><name sortKey="Cox, N J" sort="Cox, N J" uniqKey="Cox N" first="N J" last="Cox">N J Cox</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1995">1995</date><idno type="RBID">pubmed:7782782</idno><idno type="pmid">7782782</idno><idno type="doi">10.1099/0022-1317-76-6-1521</idno><idno type="wicri:Area/PubMed/Corpus">000406</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000406</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genetic stability of cold-adapted A/Leningrad/134/47/57 (H2N2) influenza virus: sequence analysis of live cold-adapted reassortant vaccine strains before and after replication in children.</title><author><name sortKey="Klimov, A I" sort="Klimov, A I" uniqKey="Klimov A" first="A I" last="Klimov">A I Klimov</name><affiliation><nlm:affiliation>Influenza Branch G-16, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Egorov, A Y" sort="Egorov, A Y" uniqKey="Egorov A" first="A Y" last="Egorov">A Y Egorov</name></author><author><name sortKey="Gushchina, M I" sort="Gushchina, M I" uniqKey="Gushchina M" first="M I" last="Gushchina">M I Gushchina</name></author><author><name sortKey="Medvedeva, T E" sort="Medvedeva, T E" uniqKey="Medvedeva T" first="T E" last="Medvedeva">T E Medvedeva</name></author><author><name sortKey="Gamble, W C" sort="Gamble, W C" uniqKey="Gamble W" first="W C" last="Gamble">W C Gamble</name></author><author><name sortKey="Rudenko, L G" sort="Rudenko, L G" uniqKey="Rudenko L" first="L G" last="Rudenko">L G Rudenko</name></author><author><name sortKey="Alexandrova, G I" sort="Alexandrova, G I" uniqKey="Alexandrova G" first="G I" last="Alexandrova">G I Alexandrova</name></author><author><name sortKey="Cox, N J" sort="Cox, N J" uniqKey="Cox N" first="N J" last="Cox">N J Cox</name></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acclimatization</term><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Child</term><term>Codon</term><term>Cold Temperature</term><term>Conserved Sequence</term><term>DNA Primers</term><term>Genome, Viral</term><term>Humans</term><term>Influenza A Virus, H2N2 Subtype</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (immunology)</term><term>Influenza A virus (physiology)</term><term>Influenza Vaccines</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction</term><term>Russia</term><term>Vaccines, Attenuated</term><term>Virus Replication</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Codon</term><term>DNA Primers</term><term>Influenza Vaccines</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Russia</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Acclimatization</term><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Child</term><term>Cold Temperature</term><term>Conserved Sequence</term><term>Genome, Viral</term><term>Humans</term><term>Influenza A Virus, H2N2 Subtype</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction</term><term>Virus Replication</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We previously reported that the A/Leningrad/134/47/57 (H2N2) cold-adapted virus (A/Len/47) used in preparing reassortant live attenuated vaccines for children acquired 14 (11 coding) mutations in genes coding for proteins other than haemagglutinin and neuraminidase during cold-adaptation. Preservation of these mutations in genomes of viruses isolated from children on the second, fifth, or eighth day after vaccination was examined by sequence analysis. The sequence data demonstrated that all nine coding mutations selected for examination were conserved in the genomes of all 11 strains investigated, indicating that the mutations accompanying cold-adaptation and attenuation of the A/Len/47 master vaccine are highly stable.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">7782782</PMID><DateCompleted><Year>1995</Year><Month>07</Month><Day>20</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1317</ISSN><JournalIssue CitedMedium="Print"><Volume>76 ( Pt 6)</Volume><PubDate><Year>1995</Year><Month>Jun</Month></PubDate></JournalIssue><Title>The Journal of general virology</Title><ISOAbbreviation>J. Gen. Virol.</ISOAbbreviation></Journal><ArticleTitle>Genetic stability of cold-adapted A/Leningrad/134/47/57 (H2N2) influenza virus: sequence analysis of live cold-adapted reassortant vaccine strains before and after replication in children.</ArticleTitle><Pagination><MedlinePgn>1521-5</MedlinePgn></Pagination><Abstract><AbstractText>We previously reported that the A/Leningrad/134/47/57 (H2N2) cold-adapted virus (A/Len/47) used in preparing reassortant live attenuated vaccines for children acquired 14 (11 coding) mutations in genes coding for proteins other than haemagglutinin and neuraminidase during cold-adaptation. Preservation of these mutations in genomes of viruses isolated from children on the second, fifth, or eighth day after vaccination was examined by sequence analysis. The sequence data demonstrated that all nine coding mutations selected for examination were conserved in the genomes of all 11 strains investigated, indicating that the mutations accompanying cold-adaptation and attenuation of the A/Len/47 master vaccine are highly stable.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Klimov</LastName><ForeName>A I</ForeName><Initials>AI</Initials><AffiliationInfo><Affiliation>Influenza Branch G-16, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Egorov</LastName><ForeName>A Y</ForeName><Initials>AY</Initials></Author><Author ValidYN="Y"><LastName>Gushchina</LastName><ForeName>M I</ForeName><Initials>MI</Initials></Author><Author ValidYN="Y"><LastName>Medvedeva</LastName><ForeName>T E</ForeName><Initials>TE</Initials></Author><Author ValidYN="Y"><LastName>Gamble</LastName><ForeName>W C</ForeName><Initials>WC</Initials></Author><Author ValidYN="Y"><LastName>Rudenko</LastName><ForeName>L G</ForeName><Initials>LG</Initials></Author><Author ValidYN="Y"><LastName>Alexandrova</LastName><ForeName>G I</ForeName><Initials>GI</Initials></Author><Author ValidYN="Y"><LastName>Cox</LastName><ForeName>N J</ForeName><Initials>NJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Gen Virol</MedlineTA><NlmUniqueID>0077340</NlmUniqueID><ISSNLinking>0022-1317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003062">Codon</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007252">Influenza Vaccines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014613">Vaccines, Attenuated</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000064" MajorTopicYN="N">Acclimatization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003062" MajorTopicYN="N">Codon</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003080" MajorTopicYN="N">Cold Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017124" MajorTopicYN="N">Conserved Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016679" MajorTopicYN="N">Genome, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053121" MajorTopicYN="Y">Influenza A Virus, H2N2 Subtype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007252" MajorTopicYN="Y">Influenza Vaccines</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012426" MajorTopicYN="N" Type="Geographic">Russia</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014613" MajorTopicYN="N">Vaccines, Attenuated</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="Y">Virus Replication</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>6</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1995</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1995</Year><Month>6</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId 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