Expression of MMP-2, 9 and 13 in newly formed bone after sinus augmentation using inorganic bovine bone in human
Identifieur interne : 004B06 ( Main/Merge ); précédent : 004B05; suivant : 004B07Expression of MMP-2, 9 and 13 in newly formed bone after sinus augmentation using inorganic bovine bone in human
Auteurs : J. Bassil [Liban] ; K. Senni [France] ; S. Changotade [France] ; B. Baroukh [France] ; C. Kassis [Liban] ; N. Naaman [Liban] ; G. Godeau [France]Source :
- Journal of periodontal research [ 0022-3484 ] ; 2011.
Descripteurs français
- Pascal (Inist)
- Wicri :
English descriptors
- KwdEn :
- Bone, Bone graft, Bovine, Human, Stomatology.
Abstract
Background and Objective: The aim of the present study was to analyse the expression of MMP-2, MMP-9 and MMP-13 in newly formed bone following maxillary sinus augmentation using inorganic bovine bone substitute, because these MMPs play a major role in bone remodeling and bone resorption. Material and Methods: Deproteinized bovine bone (Bio-Oss®) was used to fill cavities after elevating the sinus mucosa. Twenty patients with edentulous posterior maxilla were treated with 20 sinus-augmentation procedures using a two-stage technique. Forty-nine Straumann® endosseous implants were used to complete the implant-prosthetic rehabilitation. One cylinder-shaped bone biopsy from each patient was taken from the augmented maxillary region using trephine burs at the second stage of surgery, 8 months after grafting. A biopsy was also taken as a control from the upper molar region from six different patients who did not undergo the sinus procedure. All biopsies were subjected to biochemical analysis and staining for TRAP. Results: No implant losses or failures occurred. The large number of TRAP-positive multinucleated osteoclasts in resorption lacunae indicated that the resorption was very active in all grafts, in contrast with the control group. Zymography and western blot analysis demonstrated a significantly increased expression of MMP-2, MMP-9 and MMP-13 in the newly formed bone compared with controls (p < 0.05). Conclusion: The quantity of osteoclastic cells and the increased expression of proteolytic enzymes suggest that 8 months after grafting, inorganic bovine bone is slowly resorbing and is the site of important remodeling of the newly formed bone by means of resorption and synthesis.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000109
- to stream PascalFrancis, to step Curation: 000591
- to stream PascalFrancis, to step Checkpoint: 000139
Links to Exploration step
Pascal:11-0481927Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Expression of MMP-2, 9 and 13 in newly formed bone after sinus augmentation using inorganic bovine bone in human</title><author><name sortKey="Bassil, J" sort="Bassil, J" uniqKey="Bassil J" first="J." last="Bassil">J. Bassil</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Liban</country><wicri:noRegion>Beirut</wicri:noRegion></affiliation></author><author><name sortKey="Senni, K" sort="Senni, K" uniqKey="Senni K" first="K." last="Senni">K. Senni</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>LBPS-CSPBAT/ UMR CNRS 7244, Paris 13 University</s1><s2>Bobigny</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Bobigny</settlement></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Innovation Department, Seadev-Fermensys SA</s1><s2>Plouzané</s2><s3>FRA</s3><sZ>2 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Plouzané</wicri:noRegion><wicri:noRegion>Seadev-Fermensys SA</wicri:noRegion><wicri:noRegion>Plouzané</wicri:noRegion></affiliation></author><author><name sortKey="Changotade, S" sort="Changotade, S" uniqKey="Changotade S" first="S." last="Changotade">S. Changotade</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>LBPS-CSPBAT/ UMR CNRS 7244, Paris 13 University</s1><s2>Bobigny</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Bobigny</settlement></placeName></affiliation></author><author><name sortKey="Baroukh, B" sort="Baroukh, B" uniqKey="Baroukh B" first="B." last="Baroukh">B. Baroukh</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Faculty of Dental Surgery, Paris-Descartes University</s1><s2>Montrouge</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Montrouge</wicri:noRegion><wicri:noRegion>Paris-Descartes University</wicri:noRegion><wicri:noRegion>Montrouge</wicri:noRegion></affiliation></author><author><name sortKey="Kassis, C" sort="Kassis, C" uniqKey="Kassis C" first="C." last="Kassis">C. Kassis</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Liban</country><wicri:noRegion>Beirut</wicri:noRegion></affiliation></author><author><name sortKey="Naaman, N" sort="Naaman, N" uniqKey="Naaman N" first="N." last="Naaman">N. Naaman</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Liban</country><wicri:noRegion>Beirut</wicri:noRegion></affiliation></author><author><name sortKey="Godeau, G" sort="Godeau, G" uniqKey="Godeau G" first="G." last="Godeau">G. Godeau</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Faculty of Dental Surgery, Paris-Descartes University</s1><s2>Montrouge</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Montrouge</wicri:noRegion><wicri:noRegion>Paris-Descartes University</wicri:noRegion><wicri:noRegion>Montrouge</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">11-0481927</idno><date when="2011">2011</date><idno type="stanalyst">PASCAL 11-0481927 INIST</idno><idno type="RBID">Pascal:11-0481927</idno><idno type="wicri:Area/PascalFrancis/Corpus">000109</idno><idno type="wicri:Area/PascalFrancis/Curation">000591</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000139</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000139</idno><idno type="wicri:doubleKey">0022-3484:2011:Bassil J:expression:of:mmp</idno><idno type="wicri:Area/Main/Merge">004B06</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Expression of MMP-2, 9 and 13 in newly formed bone after sinus augmentation using inorganic bovine bone in human</title><author><name sortKey="Bassil, J" sort="Bassil, J" uniqKey="Bassil J" first="J." last="Bassil">J. Bassil</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Liban</country><wicri:noRegion>Beirut</wicri:noRegion></affiliation></author><author><name sortKey="Senni, K" sort="Senni, K" uniqKey="Senni K" first="K." last="Senni">K. Senni</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>LBPS-CSPBAT/ UMR CNRS 7244, Paris 13 University</s1><s2>Bobigny</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Bobigny</settlement></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Innovation Department, Seadev-Fermensys SA</s1><s2>Plouzané</s2><s3>FRA</s3><sZ>2 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Plouzané</wicri:noRegion><wicri:noRegion>Seadev-Fermensys SA</wicri:noRegion><wicri:noRegion>Plouzané</wicri:noRegion></affiliation></author><author><name sortKey="Changotade, S" sort="Changotade, S" uniqKey="Changotade S" first="S." last="Changotade">S. Changotade</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>LBPS-CSPBAT/ UMR CNRS 7244, Paris 13 University</s1><s2>Bobigny</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Bobigny</settlement></placeName></affiliation></author><author><name sortKey="Baroukh, B" sort="Baroukh, B" uniqKey="Baroukh B" first="B." last="Baroukh">B. Baroukh</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Faculty of Dental Surgery, Paris-Descartes University</s1><s2>Montrouge</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Montrouge</wicri:noRegion><wicri:noRegion>Paris-Descartes University</wicri:noRegion><wicri:noRegion>Montrouge</wicri:noRegion></affiliation></author><author><name sortKey="Kassis, C" sort="Kassis, C" uniqKey="Kassis C" first="C." last="Kassis">C. Kassis</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Liban</country><wicri:noRegion>Beirut</wicri:noRegion></affiliation></author><author><name sortKey="Naaman, N" sort="Naaman, N" uniqKey="Naaman N" first="N." last="Naaman">N. Naaman</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Liban</country><wicri:noRegion>Beirut</wicri:noRegion></affiliation></author><author><name sortKey="Godeau, G" sort="Godeau, G" uniqKey="Godeau G" first="G." last="Godeau">G. Godeau</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Faculty of Dental Surgery, Paris-Descartes University</s1><s2>Montrouge</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Montrouge</wicri:noRegion><wicri:noRegion>Paris-Descartes University</wicri:noRegion><wicri:noRegion>Montrouge</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of periodontal research</title><title level="j" type="abbreviated">J. periodontal res.</title><idno type="ISSN">0022-3484</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of periodontal research</title><title level="j" type="abbreviated">J. periodontal res.</title><idno type="ISSN">0022-3484</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bone</term><term>Bone graft</term><term>Bovine</term><term>Human</term><term>Stomatology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Os</term><term>Bovin</term><term>Homme</term><term>Stomatologie</term><term>Greffe osseuse</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Bovin</term><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background and Objective: The aim of the present study was to analyse the expression of MMP-2, MMP-9 and MMP-13 in newly formed bone following maxillary sinus augmentation using inorganic bovine bone substitute, because these MMPs play a major role in bone remodeling and bone resorption. Material and Methods: Deproteinized bovine bone (Bio-Oss®) was used to fill cavities after elevating the sinus mucosa. Twenty patients with edentulous posterior maxilla were treated with 20 sinus-augmentation procedures using a two-stage technique. Forty-nine Straumann® endosseous implants were used to complete the implant-prosthetic rehabilitation. One cylinder-shaped bone biopsy from each patient was taken from the augmented maxillary region using trephine burs at the second stage of surgery, 8 months after grafting. A biopsy was also taken as a control from the upper molar region from six different patients who did not undergo the sinus procedure. All biopsies were subjected to biochemical analysis and staining for TRAP. Results: No implant losses or failures occurred. The large number of TRAP-positive multinucleated osteoclasts in resorption lacunae indicated that the resorption was very active in all grafts, in contrast with the control group. Zymography and western blot analysis demonstrated a significantly increased expression of MMP-2, MMP-9 and MMP-13 in the newly formed bone compared with controls (p < 0.05). Conclusion: The quantity of osteoclastic cells and the increased expression of proteolytic enzymes suggest that 8 months after grafting, inorganic bovine bone is slowly resorbing and is the site of important remodeling of the newly formed bone by means of resorption and synthesis.</div></front></TEI><affiliations><list><country><li>France</li><li>Liban</li></country><region><li>Île-de-France</li></region><settlement><li>Bobigny</li></settlement></list><tree><country name="Liban"><noRegion><name sortKey="Bassil, J" sort="Bassil, J" uniqKey="Bassil J" first="J." last="Bassil">J. Bassil</name></noRegion><name sortKey="Kassis, C" sort="Kassis, C" uniqKey="Kassis C" first="C." last="Kassis">C. Kassis</name><name sortKey="Naaman, N" sort="Naaman, N" uniqKey="Naaman N" first="N." last="Naaman">N. Naaman</name></country><country name="France"><region name="Île-de-France"><name sortKey="Senni, K" sort="Senni, K" uniqKey="Senni K" first="K." last="Senni">K. Senni</name></region><name sortKey="Baroukh, B" sort="Baroukh, B" uniqKey="Baroukh B" first="B." last="Baroukh">B. Baroukh</name><name sortKey="Changotade, S" sort="Changotade, S" uniqKey="Changotade S" first="S." last="Changotade">S. Changotade</name><name sortKey="Godeau, G" sort="Godeau, G" uniqKey="Godeau G" first="G." last="Godeau">G. Godeau</name><name sortKey="Senni, K" sort="Senni, K" uniqKey="Senni K" first="K." last="Senni">K. Senni</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004B06 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 004B06 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Santé
|area= EdenteV2
|flux= Main
|étape= Merge
|type= RBID
|clé= Pascal:11-0481927
|texte= Expression of MMP-2, 9 and 13 in newly formed bone after sinus augmentation using inorganic bovine bone in human
}}
| This area was generated with Dilib version V0.6.32. |  |