Expression of MMP-2, 9 and 13 in newly formed bone after sinus augmentation using inorganic bovine bone in human
Identifieur interne : 000139 ( PascalFrancis/Checkpoint ); précédent : 000138; suivant : 000140Expression of MMP-2, 9 and 13 in newly formed bone after sinus augmentation using inorganic bovine bone in human
Auteurs : J. Bassil [Liban] ; K. Senni [France] ; S. Changotade [France] ; B. Baroukh [France] ; C. Kassis [Liban] ; N. Naaman [Liban] ; G. Godeau [France]Source :
- Journal of periodontal research [ 0022-3484 ] ; 2011.
Descripteurs français
- Pascal (Inist)
- Wicri :
English descriptors
- KwdEn :
- Bone, Bone graft, Bovine, Human, Stomatology.
Abstract
Background and Objective: The aim of the present study was to analyse the expression of MMP-2, MMP-9 and MMP-13 in newly formed bone following maxillary sinus augmentation using inorganic bovine bone substitute, because these MMPs play a major role in bone remodeling and bone resorption. Material and Methods: Deproteinized bovine bone (Bio-Oss®) was used to fill cavities after elevating the sinus mucosa. Twenty patients with edentulous posterior maxilla were treated with 20 sinus-augmentation procedures using a two-stage technique. Forty-nine Straumann® endosseous implants were used to complete the implant-prosthetic rehabilitation. One cylinder-shaped bone biopsy from each patient was taken from the augmented maxillary region using trephine burs at the second stage of surgery, 8 months after grafting. A biopsy was also taken as a control from the upper molar region from six different patients who did not undergo the sinus procedure. All biopsies were subjected to biochemical analysis and staining for TRAP. Results: No implant losses or failures occurred. The large number of TRAP-positive multinucleated osteoclasts in resorption lacunae indicated that the resorption was very active in all grafts, in contrast with the control group. Zymography and western blot analysis demonstrated a significantly increased expression of MMP-2, MMP-9 and MMP-13 in the newly formed bone compared with controls (p < 0.05). Conclusion: The quantity of osteoclastic cells and the increased expression of proteolytic enzymes suggest that 8 months after grafting, inorganic bovine bone is slowly resorbing and is the site of important remodeling of the newly formed bone by means of resorption and synthesis.
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Senni</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>LBPS-CSPBAT/ UMR CNRS 7244, Paris 13 University</s1><s2>Bobigny</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Bobigny</settlement></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Innovation Department, Seadev-Fermensys SA</s1><s2>Plouzané</s2><s3>FRA</s3><sZ>2 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Plouzané</wicri:noRegion><wicri:noRegion>Seadev-Fermensys SA</wicri:noRegion><wicri:noRegion>Plouzané</wicri:noRegion></affiliation></author><author><name sortKey="Changotade, S" sort="Changotade, S" uniqKey="Changotade S" first="S." last="Changotade">S. Changotade</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>LBPS-CSPBAT/ UMR CNRS 7244, Paris 13 University</s1><s2>Bobigny</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Bobigny</settlement></placeName></affiliation></author><author><name sortKey="Baroukh, B" sort="Baroukh, B" uniqKey="Baroukh B" first="B." last="Baroukh">B. Baroukh</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Faculty of Dental Surgery, Paris-Descartes University</s1><s2>Montrouge</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Montrouge</wicri:noRegion><wicri:noRegion>Paris-Descartes University</wicri:noRegion><wicri:noRegion>Montrouge</wicri:noRegion></affiliation></author><author><name sortKey="Kassis, C" sort="Kassis, C" uniqKey="Kassis C" first="C." last="Kassis">C. Kassis</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Liban</country><wicri:noRegion>Beirut</wicri:noRegion></affiliation></author><author><name sortKey="Naaman, N" sort="Naaman, N" uniqKey="Naaman N" first="N." last="Naaman">N. Naaman</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Liban</country><wicri:noRegion>Beirut</wicri:noRegion></affiliation></author><author><name sortKey="Godeau, G" sort="Godeau, G" uniqKey="Godeau G" first="G." last="Godeau">G. Godeau</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Faculty of Dental Surgery, Paris-Descartes University</s1><s2>Montrouge</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Montrouge</wicri:noRegion><wicri:noRegion>Paris-Descartes University</wicri:noRegion><wicri:noRegion>Montrouge</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">11-0481927</idno><date when="2011">2011</date><idno type="stanalyst">PASCAL 11-0481927 INIST</idno><idno type="RBID">Pascal:11-0481927</idno><idno type="wicri:Area/PascalFrancis/Corpus">000109</idno><idno type="wicri:Area/PascalFrancis/Curation">000591</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000139</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000139</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Expression of MMP-2, 9 and 13 in newly formed bone after sinus augmentation using inorganic bovine bone in human</title><author><name sortKey="Bassil, J" sort="Bassil, J" uniqKey="Bassil J" first="J." last="Bassil">J. Bassil</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Liban</country><wicri:noRegion>Beirut</wicri:noRegion></affiliation></author><author><name sortKey="Senni, K" sort="Senni, K" uniqKey="Senni K" first="K." last="Senni">K. Senni</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>LBPS-CSPBAT/ UMR CNRS 7244, Paris 13 University</s1><s2>Bobigny</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Bobigny</settlement></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Innovation Department, Seadev-Fermensys SA</s1><s2>Plouzané</s2><s3>FRA</s3><sZ>2 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Plouzané</wicri:noRegion><wicri:noRegion>Seadev-Fermensys SA</wicri:noRegion><wicri:noRegion>Plouzané</wicri:noRegion></affiliation></author><author><name sortKey="Changotade, S" sort="Changotade, S" uniqKey="Changotade S" first="S." last="Changotade">S. Changotade</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>LBPS-CSPBAT/ UMR CNRS 7244, Paris 13 University</s1><s2>Bobigny</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Bobigny</settlement></placeName></affiliation></author><author><name sortKey="Baroukh, B" sort="Baroukh, B" uniqKey="Baroukh B" first="B." last="Baroukh">B. Baroukh</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Faculty of Dental Surgery, Paris-Descartes University</s1><s2>Montrouge</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Montrouge</wicri:noRegion><wicri:noRegion>Paris-Descartes University</wicri:noRegion><wicri:noRegion>Montrouge</wicri:noRegion></affiliation></author><author><name sortKey="Kassis, C" sort="Kassis, C" uniqKey="Kassis C" first="C." last="Kassis">C. Kassis</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Liban</country><wicri:noRegion>Beirut</wicri:noRegion></affiliation></author><author><name sortKey="Naaman, N" sort="Naaman, N" uniqKey="Naaman N" first="N." last="Naaman">N. Naaman</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Liban</country><wicri:noRegion>Beirut</wicri:noRegion></affiliation></author><author><name sortKey="Godeau, G" sort="Godeau, G" uniqKey="Godeau G" first="G." last="Godeau">G. Godeau</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Faculty of Dental Surgery, Paris-Descartes University</s1><s2>Montrouge</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>Montrouge</wicri:noRegion><wicri:noRegion>Paris-Descartes University</wicri:noRegion><wicri:noRegion>Montrouge</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of periodontal research</title><title level="j" type="abbreviated">J. periodontal res.</title><idno type="ISSN">0022-3484</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of periodontal research</title><title level="j" type="abbreviated">J. periodontal res.</title><idno type="ISSN">0022-3484</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bone</term><term>Bone graft</term><term>Bovine</term><term>Human</term><term>Stomatology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Os</term><term>Bovin</term><term>Homme</term><term>Stomatologie</term><term>Greffe osseuse</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Bovin</term><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background and Objective: The aim of the present study was to analyse the expression of MMP-2, MMP-9 and MMP-13 in newly formed bone following maxillary sinus augmentation using inorganic bovine bone substitute, because these MMPs play a major role in bone remodeling and bone resorption. Material and Methods: Deproteinized bovine bone (Bio-Oss®) was used to fill cavities after elevating the sinus mucosa. Twenty patients with edentulous posterior maxilla were treated with 20 sinus-augmentation procedures using a two-stage technique. Forty-nine Straumann® endosseous implants were used to complete the implant-prosthetic rehabilitation. One cylinder-shaped bone biopsy from each patient was taken from the augmented maxillary region using trephine burs at the second stage of surgery, 8 months after grafting. A biopsy was also taken as a control from the upper molar region from six different patients who did not undergo the sinus procedure. All biopsies were subjected to biochemical analysis and staining for TRAP. Results: No implant losses or failures occurred. The large number of TRAP-positive multinucleated osteoclasts in resorption lacunae indicated that the resorption was very active in all grafts, in contrast with the control group. Zymography and western blot analysis demonstrated a significantly increased expression of MMP-2, MMP-9 and MMP-13 in the newly formed bone compared with controls (p < 0.05). Conclusion: The quantity of osteoclastic cells and the increased expression of proteolytic enzymes suggest that 8 months after grafting, inorganic bovine bone is slowly resorbing and is the site of important remodeling of the newly formed bone by means of resorption and synthesis.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-3484</s0></fA01><fA03 i2="1"><s0>J. periodontal res.</s0></fA03><fA05><s2>46</s2></fA05><fA06><s2>6</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Expression of MMP-2, 9 and 13 in newly formed bone after sinus augmentation using inorganic bovine bone in human</s1></fA08><fA11 i1="01" i2="1"><s1>BASSIL (J.)</s1></fA11><fA11 i1="02" i2="1"><s1>SENNI (K.)</s1></fA11><fA11 i1="03" i2="1"><s1>CHANGOTADE (S.)</s1></fA11><fA11 i1="04" i2="1"><s1>BAROUKH (B.)</s1></fA11><fA11 i1="05" i2="1"><s1>KASSIS (C.)</s1></fA11><fA11 i1="06" i2="1"><s1>NAAMAN (N.)</s1></fA11><fA11 i1="07" i2="1"><s1>GODEAU (G.)</s1></fA11><fA14 i1="01"><s1>Faculty of Dental Medicine, St Joseph University</s1><s2>Beirut</s2><s3>LBN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></fA14><fA14 i1="02"><s1>LBPS-CSPBAT/ UMR CNRS 7244, Paris 13 University</s1><s2>Bobigny</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></fA14><fA14 i1="03"><s1>Innovation Department, Seadev-Fermensys SA</s1><s2>Plouzané</s2><s3>FRA</s3><sZ>2 aut.</sZ></fA14><fA14 i1="04"><s1>Faculty of Dental Surgery, Paris-Descartes University</s1><s2>Montrouge</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ></fA14><fA20><s1>756-762</s1></fA20><fA21><s1>2011</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>15072</s2><s5>354000505568370160</s5></fA43><fA44><s0>0000</s0><s1>© 2011 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>47 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>11-0481927</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Journal of periodontal research</s0></fA64><fA66 i1="01"><s0>GBR</s0></fA66><fC01 i1="01" l="ENG"><s0>Background and Objective: The aim of the present study was to analyse the expression of MMP-2, MMP-9 and MMP-13 in newly formed bone following maxillary sinus augmentation using inorganic bovine bone substitute, because these MMPs play a major role in bone remodeling and bone resorption. Material and Methods: Deproteinized bovine bone (Bio-Oss®) was used to fill cavities after elevating the sinus mucosa. Twenty patients with edentulous posterior maxilla were treated with 20 sinus-augmentation procedures using a two-stage technique. Forty-nine Straumann® endosseous implants were used to complete the implant-prosthetic rehabilitation. One cylinder-shaped bone biopsy from each patient was taken from the augmented maxillary region using trephine burs at the second stage of surgery, 8 months after grafting. A biopsy was also taken as a control from the upper molar region from six different patients who did not undergo the sinus procedure. All biopsies were subjected to biochemical analysis and staining for TRAP. Results: No implant losses or failures occurred. The large number of TRAP-positive multinucleated osteoclasts in resorption lacunae indicated that the resorption was very active in all grafts, in contrast with the control group. Zymography and western blot analysis demonstrated a significantly increased expression of MMP-2, MMP-9 and MMP-13 in the newly formed bone compared with controls (p < 0.05). Conclusion: The quantity of osteoclastic cells and the increased expression of proteolytic enzymes suggest that 8 months after grafting, inorganic bovine bone is slowly resorbing and is the site of important remodeling of the newly formed bone by means of resorption and synthesis.</s0></fC01><fC02 i1="01" i2="X"><s0>002B10</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Os</s0><s5>07</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Bone</s0><s5>07</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Hueso</s0><s5>07</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Bovin</s0><s5>08</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Bovine</s0><s5>08</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Bovino</s0><s5>08</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Homme</s0><s5>09</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Human</s0><s5>09</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Hombre</s0><s5>09</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Stomatologie</s0><s5>13</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Stomatology</s0><s5>13</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Estomatología</s0><s5>13</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Greffe osseuse</s0><s4>CD</s4><s5>96</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Bone graft</s0><s4>CD</s4><s5>96</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Injerto óseo</s0><s4>CD</s4><s5>96</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Artiodactyla</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Artiodactyla</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Artiodactyla</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Ungulata</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Ungulata</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Ungulata</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Vertebrata</s0><s2>NS</s2></fC07><fN21><s1>332</s1></fN21><fN44 i1="01"><s1>OTO</s1></fN44><fN82><s1>OTO</s1></fN82></pA></standard></inist><affiliations><list><country><li>France</li><li>Liban</li></country><region><li>Île-de-France</li></region><settlement><li>Bobigny</li></settlement></list><tree><country name="Liban"><noRegion><name sortKey="Bassil, J" sort="Bassil, J" uniqKey="Bassil J" first="J." last="Bassil">J. Bassil</name></noRegion><name sortKey="Kassis, C" sort="Kassis, C" uniqKey="Kassis C" first="C." last="Kassis">C. Kassis</name><name sortKey="Naaman, N" sort="Naaman, N" uniqKey="Naaman N" first="N." last="Naaman">N. Naaman</name></country><country name="France"><region name="Île-de-France"><name sortKey="Senni, K" sort="Senni, K" uniqKey="Senni K" first="K." last="Senni">K. Senni</name></region><name sortKey="Baroukh, B" sort="Baroukh, B" uniqKey="Baroukh B" first="B." last="Baroukh">B. Baroukh</name><name sortKey="Changotade, S" sort="Changotade, S" uniqKey="Changotade S" first="S." last="Changotade">S. Changotade</name><name sortKey="Godeau, G" sort="Godeau, G" uniqKey="Godeau G" first="G." last="Godeau">G. Godeau</name><name sortKey="Senni, K" sort="Senni, K" uniqKey="Senni K" first="K." last="Senni">K. Senni</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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