In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus
Identifieur interne : 000250 ( Pmc/Checkpoint ); précédent : 000249; suivant : 000251In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus
Auteurs : Lan Li ; Xiaoming Yu ; Haoming Zhang ; Haiwei Cheng ; Liting Hou ; Qisheng Zheng ; Jibo HouSource :
- Virus Genes [ 0920-8569 ] ; 2019.
Abstract
Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin’s inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin’s action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.
Url:
DOI: 10.1007/s11262-019-01633-7
PubMed: 30637608
PubMed Central: 7089098
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:7089098Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus</title><author><name sortKey="Li, Lan" sort="Li, Lan" uniqKey="Li L" first="Lan" last="Li">Lan Li</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Yu, Xiaoming" sort="Yu, Xiaoming" uniqKey="Yu X" first="Xiaoming" last="Yu">Xiaoming Yu</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Zhang, Haoming" sort="Zhang, Haoming" uniqKey="Zhang H" first="Haoming" last="Zhang">Haoming Zhang</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Cheng, Haiwei" sort="Cheng, Haiwei" uniqKey="Cheng H" first="Haiwei" last="Cheng">Haiwei Cheng</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Hou, Liting" sort="Hou, Liting" uniqKey="Hou L" first="Liting" last="Hou">Liting Hou</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Zheng, Qisheng" sort="Zheng, Qisheng" uniqKey="Zheng Q" first="Qisheng" last="Zheng">Qisheng Zheng</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Hou, Jibo" sort="Hou, Jibo" uniqKey="Hou J" first="Jibo" last="Hou">Jibo Hou</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2">Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">225009 Jiangsu China</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">30637608</idno><idno type="pmc">7089098</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089098</idno><idno type="RBID">PMC:7089098</idno><idno type="doi">10.1007/s11262-019-01633-7</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000356</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000356</idno><idno type="wicri:Area/Pmc/Curation">000356</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000356</idno><idno type="wicri:Area/Pmc/Checkpoint">000250</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000250</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus</title><author><name sortKey="Li, Lan" sort="Li, Lan" uniqKey="Li L" first="Lan" last="Li">Lan Li</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Yu, Xiaoming" sort="Yu, Xiaoming" uniqKey="Yu X" first="Xiaoming" last="Yu">Xiaoming Yu</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Zhang, Haoming" sort="Zhang, Haoming" uniqKey="Zhang H" first="Haoming" last="Zhang">Haoming Zhang</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Cheng, Haiwei" sort="Cheng, Haiwei" uniqKey="Cheng H" first="Haiwei" last="Cheng">Haiwei Cheng</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Hou, Liting" sort="Hou, Liting" uniqKey="Hou L" first="Liting" last="Hou">Liting Hou</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Zheng, Qisheng" sort="Zheng, Qisheng" uniqKey="Zheng Q" first="Qisheng" last="Zheng">Qisheng Zheng</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Hou, Jibo" sort="Hou, Jibo" uniqKey="Hou J" first="Jibo" last="Hou">Jibo Hou</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2">Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">225009 Jiangsu China</wicri:noCountry></affiliation></author></analytic><series><title level="j">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin’s inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin’s action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Mori, T" uniqKey="Mori T">T Mori</name></author><author><name sortKey="O Eefe, Br" uniqKey="O Eefe B">BR O’Keefe</name></author><author><name sortKey="Sowder, Rc" uniqKey="Sowder R">RC Sowder</name></author><author><name sortKey="Bringans, S" uniqKey="Bringans S">S Bringans</name></author><author><name sortKey="Gardella, R" uniqKey="Gardella R">R Gardella</name></author><author><name sortKey="Berg, S" uniqKey="Berg S">S Berg</name></author><author><name sortKey="Cochran, P" uniqKey="Cochran P">P Cochran</name></author><author><name sortKey="Turpin, Ja" uniqKey="Turpin J">JA Turpin</name></author><author><name sortKey="Buckheit, Rw" uniqKey="Buckheit R">RW Buckheit</name></author><author><name sortKey="Mcmahon, Jb" uniqKey="Mcmahon J">JB McMahon</name></author><author><name sortKey="Boyd, Mr" uniqKey="Boyd M">MR Boyd</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lusvarghi, S" uniqKey="Lusvarghi S">S Lusvarghi</name></author><author><name sortKey="Bewley, Ca" uniqKey="Bewley C">CA Bewley</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Ziolkowska, N" uniqKey="Ziolkowska N">N Ziolkowska</name></author><author><name sortKey="O Eefe, Br" uniqKey="O Eefe B">BR O’Keefe</name></author><author><name sortKey="Mori, T" uniqKey="Mori T">T Mori</name></author><author><name sortKey="Zhu, C" uniqKey="Zhu C">C Zhu</name></author><author><name sortKey="Giomarelli, B" uniqKey="Giomarelli B">B Giomarelli</name></author><author><name sortKey="Vojdani, F" uniqKey="Vojdani F">F Vojdani</name></author><author><name sortKey="Palmer, K" uniqKey="Palmer K">K Palmer</name></author><author><name sortKey="Mcmahon, Jb" uniqKey="Mcmahon J">JB McMahon</name></author><author><name sortKey="Wlodawer, A" uniqKey="Wlodawer A">A Wlodawer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Millet, Jk" uniqKey="Millet J">JK Millet</name></author><author><name sortKey="Seron, K" uniqKey="Seron K">K Séron</name></author><author><name sortKey="Labitt, Rn" uniqKey="Labitt R">RN Labitt</name></author><author><name sortKey="Danneels, A" uniqKey="Danneels A">A Danneels</name></author><author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name></author><author><name sortKey="Whittaker, Gr" uniqKey="Whittaker G">GR Whittaker</name></author><author><name sortKey="Dubuisson, J" uniqKey="Dubuisson J">J Dubuisson</name></author><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S Belouzard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takebe, Y" uniqKey="Takebe Y">Y Takebe</name></author><author><name sortKey="Saucedo, Cj" uniqKey="Saucedo C">CJ Saucedo</name></author><author><name sortKey="Lund, G" uniqKey="Lund G">G Lund</name></author><author><name sortKey="Uenishi, R" uniqKey="Uenishi R">R Uenishi</name></author><author><name sortKey="Hase, S" uniqKey="Hase S">S Hase</name></author><author><name sortKey="Tsuchiura, T" uniqKey="Tsuchiura T">T Tsuchiura</name></author><author><name sortKey="Kneteman, N" uniqKey="Kneteman N">N Kneteman</name></author><author><name sortKey="Ramessar, K" uniqKey="Ramessar K">K Ramessar</name></author><author><name sortKey="Tyrrell, Dl" uniqKey="Tyrrell D">DL Tyrrell</name></author><author><name sortKey="Shirakura, M" uniqKey="Shirakura M">M Shirakura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nixon, B" uniqKey="Nixon B">B Nixon</name></author><author><name sortKey="Stefanidou, M" uniqKey="Stefanidou M">M Stefanidou</name></author><author><name sortKey="Mesquita, Pm" uniqKey="Mesquita P">PM Mesquita</name></author><author><name sortKey="Fakioglu, E" uniqKey="Fakioglu E">E Fakioglu</name></author><author><name sortKey="Segarra, T" uniqKey="Segarra T">T Segarra</name></author><author><name sortKey="Rohan, L" uniqKey="Rohan L">L Rohan</name></author><author><name sortKey="Halford, W" uniqKey="Halford W">W Halford</name></author><author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name></author><author><name sortKey="Herold, Bc" uniqKey="Herold B">BC Herold</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ishag, Hz" uniqKey="Ishag H">HZ Ishag</name></author><author><name sortKey="Li, C" uniqKey="Li C">C Li</name></author><author><name sortKey="Wang, F" uniqKey="Wang F">F Wang</name></author><author><name sortKey="Mao, X" uniqKey="Mao X">X Mao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ishag, Hza" uniqKey="Ishag H">HZA Ishag</name></author><author><name sortKey="Li, C" uniqKey="Li C">C Li</name></author><author><name sortKey="Huang, L" uniqKey="Huang L">L Huang</name></author><author><name sortKey="Sun, Mx" uniqKey="Sun M">MX Sun</name></author><author><name sortKey="Wang, F" uniqKey="Wang F">F Wang</name></author><author><name sortKey="Ni, B" uniqKey="Ni B">B Ni</name></author><author><name sortKey="Malik, T" uniqKey="Malik T">T Malik</name></author><author><name sortKey="Chen, Py" uniqKey="Chen P">PY Chen</name></author><author><name sortKey="Mao, X" uniqKey="Mao X">X Mao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Levendosky, K" uniqKey="Levendosky K">K Levendosky</name></author><author><name sortKey="Mizenina, O" uniqKey="Mizenina O">O Mizenina</name></author><author><name sortKey="Martinelli, E" uniqKey="Martinelli E">E Martinelli</name></author><author><name sortKey="Jeanpierre, N" uniqKey="Jeanpierre N">N Jeanpierre</name></author><author><name sortKey="Kizima, L" uniqKey="Kizima L">L Kizima</name></author><author><name sortKey="Rodriguez, A" uniqKey="Rodriguez A">A Rodriguez</name></author><author><name sortKey="Kleinbeck, K" uniqKey="Kleinbeck K">K Kleinbeck</name></author><author><name sortKey="Bonnaire, T" uniqKey="Bonnaire T">T Bonnaire</name></author><author><name sortKey="Robbiani, M" uniqKey="Robbiani M">M Robbiani</name></author><author><name sortKey="Zydowsky, Tm" uniqKey="Zydowsky T">TM Zydowsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kouokam, Jc" uniqKey="Kouokam J">JC Kouokam</name></author><author><name sortKey="Huskens, D" uniqKey="Huskens D">D Huskens</name></author><author><name sortKey="Schols, D" uniqKey="Schols D">D Schols</name></author><author><name sortKey="Johannemann, A" uniqKey="Johannemann A">A Johannemann</name></author><author><name sortKey="Riedell, Sk" uniqKey="Riedell S">SK Riedell</name></author><author><name sortKey="Walter, W" uniqKey="Walter W">W Walter</name></author><author><name sortKey="Walker, Jm" uniqKey="Walker J">JM Walker</name></author><author><name sortKey="Matoba, N" uniqKey="Matoba N">N Matoba</name></author><author><name sortKey="O Eefe, Br" uniqKey="O Eefe B">BR O’Keefe</name></author><author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fuqua, Jl" uniqKey="Fuqua J">JL Fuqua</name></author><author><name sortKey="Wanga, V" uniqKey="Wanga V">V Wanga</name></author><author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moncla, Bj" uniqKey="Moncla B">BJ Moncla</name></author><author><name sortKey="Pryke, K" uniqKey="Pryke K">K Pryke</name></author><author><name sortKey="Rohan, Lc" uniqKey="Rohan L">LC Rohan</name></author><author><name sortKey="Graebing, Pw" uniqKey="Graebing P">PW Graebing</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Diel, Dg" uniqKey="Diel D">DG Diel</name></author><author><name sortKey="Lawson, S" uniqKey="Lawson S">S Lawson</name></author><author><name sortKey="Okda, F" uniqKey="Okda F">F Okda</name></author><author><name sortKey="Singrey, A" uniqKey="Singrey A">A Singrey</name></author><author><name sortKey="Clement, T" uniqKey="Clement T">T Clement</name></author><author><name sortKey="Fernandes, Mh" uniqKey="Fernandes M">MH Fernandes</name></author><author><name sortKey="Christopher Hennings, J" uniqKey="Christopher Hennings J">J Christopher-Hennings</name></author><author><name sortKey="Nelson, Ea" uniqKey="Nelson E">EA Nelson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author><author><name sortKey="Fang, L" uniqKey="Fang L">L Fang</name></author><author><name sortKey="Xiao, S" uniqKey="Xiao S">S Xiao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Hj" uniqKey="Li H">HJ Li</name></author><author><name sortKey="Gao, Ds" uniqKey="Gao D">DS Gao</name></author><author><name sortKey="Li, Yt" uniqKey="Li Y">YT Li</name></author><author><name sortKey="Wang, Ys" uniqKey="Wang Y">YS Wang</name></author><author><name sortKey="Liu, Hy" uniqKey="Liu H">HY Liu</name></author><author><name sortKey="Zhao, J" uniqKey="Zhao J">J Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, C" uniqKey="Lee C">C Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hwajung, C" uniqKey="Hwajung C">C Hwajung</name></author><author><name sortKey="Jinhee, K" uniqKey="Jinhee K">K Jinhee</name></author><author><name sortKey="Choonghwan, L" uniqKey="Choonghwan L">L Choonghwan</name></author><author><name sortKey="Youngjoon, A" uniqKey="Youngjoon A">A Youngjoon</name></author><author><name sortKey="Song, Jh" uniqKey="Song J">JH Song</name></author><author><name sortKey="Seunghwa, B" uniqKey="Seunghwa B">B Seunghwa</name></author><author><name sortKey="Durhan, K" uniqKey="Durhan K">K Durhan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Q" uniqKey="Yang Q">Q Yang</name></author><author><name sortKey="Gao, L" uniqKey="Gao L">L Gao</name></author><author><name sortKey="Si, J" uniqKey="Si J">J Si</name></author><author><name sortKey="Sun, Y" uniqKey="Sun Y">Y Sun</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author><author><name sortKey="Cao, L" uniqKey="Cao L">L Cao</name></author><author><name sortKey="Feng, Wh" uniqKey="Feng W">WH Feng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Keirstead, Nd" uniqKey="Keirstead N">ND Keirstead</name></author><author><name sortKey="Lee, C" uniqKey="Lee C">C Lee</name></author><author><name sortKey="Yoo, D" uniqKey="Yoo D">D Yoo</name></author><author><name sortKey="Brooks, As" uniqKey="Brooks A">AS Brooks</name></author><author><name sortKey="Hayes, Ma" uniqKey="Hayes M">MA Hayes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, P" uniqKey="Zhu P">P Zhu</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author><author><name sortKey="Bess, J" uniqKey="Bess J">J Bess</name></author><author><name sortKey="Chertova, E" uniqKey="Chertova E">E Chertova</name></author><author><name sortKey="Lifson, Jd" uniqKey="Lifson J">JD Lifson</name></author><author><name sortKey="Grise, H" uniqKey="Grise H">H Grisé</name></author><author><name sortKey="Ofek, Ga" uniqKey="Ofek G">GA Ofek</name></author><author><name sortKey="Taylor, Ka" uniqKey="Taylor K">KA Taylor</name></author><author><name sortKey="Roux, Kh" uniqKey="Roux K">KH Roux</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="O Eefe, Br" uniqKey="O Eefe B">BR O’Keefe</name></author><author><name sortKey="Giomarelli, B" uniqKey="Giomarelli B">B Giomarelli</name></author><author><name sortKey="Barnard, Dl" uniqKey="Barnard D">DL Barnard</name></author><author><name sortKey="Shenoy, Sr" uniqKey="Shenoy S">SR Shenoy</name></author><author><name sortKey="Chan, Pks" uniqKey="Chan P">PKS Chan</name></author><author><name sortKey="Mcmahon, Jb" uniqKey="Mcmahon J">JB Mcmahon</name></author><author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name></author><author><name sortKey="Barnett, Bw" uniqKey="Barnett B">BW Barnett</name></author><author><name sortKey="Meyerholz, Dk" uniqKey="Meyerholz D">DK Meyerholz</name></author><author><name sortKey="Wohlfordlenane, Cl" uniqKey="Wohlfordlenane C">CL Wohlfordlenane</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meuleman, P" uniqKey="Meuleman P">P Meuleman</name></author><author><name sortKey="Albecka, A" uniqKey="Albecka A">A Albecka</name></author><author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S Belouzard</name></author><author><name sortKey="Vercauteren, K" uniqKey="Vercauteren K">K Vercauteren</name></author><author><name sortKey="Verhoye, L" uniqKey="Verhoye L">L Verhoye</name></author><author><name sortKey="Wychowski, C" uniqKey="Wychowski C">C Wychowski</name></author><author><name sortKey="Lerouxroels, G" uniqKey="Lerouxroels G">G Lerouxroels</name></author><author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name></author><author><name sortKey="Dubuisson, J" uniqKey="Dubuisson J">J Dubuisson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barton, C" uniqKey="Barton C">C Barton</name></author><author><name sortKey="Kouokam, J" uniqKey="Kouokam J">J Kouokam</name></author><author><name sortKey="Hurst, H" uniqKey="Hurst H">H Hurst</name></author><author><name sortKey="Palmer, K" uniqKey="Palmer K">K Palmer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barton, C" uniqKey="Barton C">C Barton</name></author><author><name sortKey="Kouokam, Jc" uniqKey="Kouokam J">JC Kouokam</name></author><author><name sortKey="Lasnik, Ab" uniqKey="Lasnik A">AB Lasnik</name></author><author><name sortKey="Foreman, O" uniqKey="Foreman O">O Foreman</name></author><author><name sortKey="Cambon, A" uniqKey="Cambon A">A Cambon</name></author><author><name sortKey="Brock, G" uniqKey="Brock G">G Brock</name></author><author><name sortKey="Montefiori, Dc" uniqKey="Montefiori D">DC Montefiori</name></author><author><name sortKey="Vojdani, F" uniqKey="Vojdani F">F Vojdani</name></author><author><name sortKey="Mccormick, Aa" uniqKey="Mccormick A">AA Mccormick</name></author><author><name sortKey="O Eefe, Br" uniqKey="O Eefe B">BR O’Keefe</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virus Genes</journal-id><journal-id journal-id-type="iso-abbrev">Virus Genes</journal-id><journal-title-group><journal-title>Virus Genes</journal-title></journal-title-group><issn pub-type="ppub">0920-8569</issn><issn pub-type="epub">1572-994X</issn><publisher><publisher-name>Springer US</publisher-name><publisher-loc>New York</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">30637608</article-id><article-id pub-id-type="pmc">7089098</article-id><article-id pub-id-type="publisher-id">1633</article-id><article-id pub-id-type="doi">10.1007/s11262-019-01633-7</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus</article-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Li</surname><given-names>Lan</given-names></name><address><email>lanli99@foxmail.com</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Yu</surname><given-names>Xiaoming</given-names></name><address><email>yxm123abc@126.com</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Haoming</given-names></name><address><email>7231022@126.com</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Cheng</surname><given-names>Haiwei</given-names></name><address><email>chw5673@126.com</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hou</surname><given-names>Liting</given-names></name><address><email>houxyzmn@163.com</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Zheng</surname><given-names>Qisheng</given-names></name><address><email>immun_tech@163.com</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Hou</surname><given-names>Jibo</given-names></name><address><email>houjiboccvv@163.com</email></address><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</aff><aff id="Aff2"><label>2</label>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009 Jiangsu China</aff></contrib-group><author-notes><fn fn-type="com"><p>Edited by Zhen F. Fu.</p></fn></author-notes><pub-date pub-type="epub"><day>14</day><month>1</month><year>2019</year></pub-date><pub-date pub-type="ppub"><year>2019</year></pub-date><volume>55</volume><issue>2</issue><fpage>174</fpage><lpage>181</lpage><history><date date-type="received"><day>29</day><month>9</month><year>2018</year></date><date date-type="accepted"><day>2</day><month>1</month><year>2019</year></date></history><permissions><copyright-statement>© Springer Science+Business Media, LLC, part of Springer Nature 2019</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><p id="Par1">Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin’s inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin’s action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Griffithsin</kwd><kwd>Antiviral</kwd><kwd>Porcine epidemic diarrhea virus</kwd><kwd>Lectin</kwd></kwd-group><funding-group><award-group><funding-source><institution>Special Fund for Agro-scientific Research in the Public Interest (CN)</institution></funding-source><award-id>201303046</award-id><principal-award-recipient><name><surname>Hou</surname><given-names>Jibo</given-names></name></principal-award-recipient></award-group></funding-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer Science+Business Media, LLC, part of Springer Nature 2019</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Cheng, Haiwei" sort="Cheng, Haiwei" uniqKey="Cheng H" first="Haiwei" last="Cheng">Haiwei Cheng</name><name sortKey="Hou, Jibo" sort="Hou, Jibo" uniqKey="Hou J" first="Jibo" last="Hou">Jibo Hou</name><name sortKey="Hou, Liting" sort="Hou, Liting" uniqKey="Hou L" first="Liting" last="Hou">Liting Hou</name><name sortKey="Li, Lan" sort="Li, Lan" uniqKey="Li L" first="Lan" last="Li">Lan Li</name><name sortKey="Yu, Xiaoming" sort="Yu, Xiaoming" uniqKey="Yu X" first="Xiaoming" last="Yu">Xiaoming Yu</name><name sortKey="Zhang, Haoming" sort="Zhang, Haoming" uniqKey="Zhang H" first="Haoming" last="Zhang">Haoming Zhang</name><name sortKey="Zheng, Qisheng" sort="Zheng, Qisheng" uniqKey="Zheng Q" first="Qisheng" last="Zheng">Qisheng Zheng</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000250 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000250 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:7089098
|texte= In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:30637608" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a CovidV2
| This area was generated with Dilib version V0.6.33. |  |