In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus
Identifieur interne : 000356 ( Pmc/Curation ); précédent : 000355; suivant : 000357In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus
Auteurs : Lan Li ; Xiaoming Yu ; Haoming Zhang ; Haiwei Cheng ; Liting Hou ; Qisheng Zheng ; Jibo HouSource :
- Virus Genes [ 0920-8569 ] ; 2019.
Abstract
Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin’s inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin’s action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.
Url:
DOI: 10.1007/s11262-019-01633-7
PubMed: 30637608
PubMed Central: 7089098
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<front><div type="abstract" xml:lang="en"><p id="Par1">Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin’s inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin’s action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.</p>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virus Genes</journal-id>
<journal-id journal-id-type="iso-abbrev">Virus Genes</journal-id>
<journal-title-group><journal-title>Virus Genes</journal-title>
</journal-title-group>
<issn pub-type="ppub">0920-8569</issn>
<issn pub-type="epub">1572-994X</issn>
<publisher><publisher-name>Springer US</publisher-name>
<publisher-loc>New York</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">30637608</article-id>
<article-id pub-id-type="pmc">7089098</article-id>
<article-id pub-id-type="publisher-id">1633</article-id>
<article-id pub-id-type="doi">10.1007/s11262-019-01633-7</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Li</surname>
<given-names>Lan</given-names>
</name>
<address><email>lanli99@foxmail.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author" equal-contrib="yes"><name><surname>Yu</surname>
<given-names>Xiaoming</given-names>
</name>
<address><email>yxm123abc@126.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Haoming</given-names>
</name>
<address><email>7231022@126.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Cheng</surname>
<given-names>Haiwei</given-names>
</name>
<address><email>chw5673@126.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hou</surname>
<given-names>Liting</given-names>
</name>
<address><email>houxyzmn@163.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Zheng</surname>
<given-names>Qisheng</given-names>
</name>
<address><email>immun_tech@163.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Hou</surname>
<given-names>Jibo</given-names>
</name>
<address><email>houjiboccvv@163.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<aff id="Aff1"><label>1</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</aff>
<aff id="Aff2"><label>2</label>
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009 Jiangsu China</aff>
</contrib-group>
<author-notes><fn fn-type="com"><p>Edited by Zhen F. Fu.</p>
</fn>
</author-notes>
<pub-date pub-type="epub"><day>14</day>
<month>1</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="ppub"><year>2019</year>
</pub-date>
<volume>55</volume>
<issue>2</issue>
<fpage>174</fpage>
<lpage>181</lpage>
<history><date date-type="received"><day>29</day>
<month>9</month>
<year>2018</year>
</date>
<date date-type="accepted"><day>2</day>
<month>1</month>
<year>2019</year>
</date>
</history>
<permissions><copyright-statement>© Springer Science+Business Media, LLC, part of Springer Nature 2019</copyright-statement>
<license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p>
</license>
</permissions>
<abstract id="Abs1"><p id="Par1">Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin’s inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin’s action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.</p>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>Griffithsin</kwd>
<kwd>Antiviral</kwd>
<kwd>Porcine epidemic diarrhea virus</kwd>
<kwd>Lectin</kwd>
</kwd-group>
<funding-group><award-group><funding-source><institution>Special Fund for Agro-scientific Research in the Public Interest (CN)</institution>
</funding-source>
<award-id>201303046</award-id>
<principal-award-recipient><name><surname>Hou</surname>
<given-names>Jibo</given-names>
</name>
</principal-award-recipient>
</award-group>
</funding-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© Springer Science+Business Media, LLC, part of Springer Nature 2019</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
</record>
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