CovidV2, Ncbi, Merge, bibRecord, 001695

Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies

Identifieur interne : 001695 ( Ncbi/Merge ); précédent : 001694; suivant : 001696

Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies

Auteurs : H. Kasai ; E. Morita ; K. Hatakeyama ; K. Sugiyama

Source :

Archives of Virology [ 0304-8608 ] ; 1998.

RBID : PMC:7086726

Descripteurs français

KwdFr :
- Animaux, Anticorps monoclonaux (immunologie), Coronavirus (), Coronavirus (physiologie), Hémagglutinines virales (analyse), Hémagglutinines virales (immunologie), Hémagglutinines virales (physiologie), Protéines de fusion virale, Protéines virales (analyse), Protéines virales (immunologie), Protéines virales (physiologie), Souris.
MESH :
- analyse : Hémagglutinines virales, Protéines virales.
- immunologie : Anticorps monoclonaux, Hémagglutinines virales, Protéines virales.
- physiologie : Coronavirus, Hémagglutinines virales, Protéines virales.
- Animaux, Coronavirus, Protéines de fusion virale, Souris.

English descriptors

KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Coronavirus (chemistry), Coronavirus (physiology), Hemagglutinins, Viral (analysis), Hemagglutinins, Viral (immunology), Hemagglutinins, Viral (physiology), Mice, Viral Fusion Proteins, Viral Proteins (analysis), Viral Proteins (immunology), Viral Proteins (physiology).
MESH :
- chemical , analysis : Hemagglutinins, Viral, Viral Proteins.
- chemical , immunology : Antibodies, Monoclonal, Hemagglutinins, Viral, Viral Proteins.
- chemistry : Coronavirus.
- physiology : Coronavirus, Hemagglutinins, Viral, Viral Proteins.
- Animals, Mice, Viral Fusion Proteins.

Abstract

Summary

We analyzed the characteristics of seven monoclonal antibodies (mAbs) raised against purified HE (hemagglutinin-esterase) glycoprotein of the murine coronavirus DVIM (diarrhea virus of infant mice). Immunocrossreaction of these mAbs with JHM and/or MHV-S suggest that antigenic epitopes of HE of DVIM are similar to those of JHM and/or MHV-S. Four mAbs (1b4, 3Aff28, 4c19, 10b7), designated as group A mAbs, strongly inhibited both HA and AE activities. On the other hand, three mAbs (5Aff3, 6Aff6, 13Aff4), referred to as group B, had a comparatively weak HA inhibition activity. These results indicate that the antigenic epitopes of this glycoprotein can be classified into at least two groups and that the functional sites of HA and AE activities are similar but not identical. Neutralizing activity was shown in group A mAbs exclusively, suggesting that the ratio of HA and/or AE activities may play important roles in the cell fusion activity of DVIM-infected cells.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086726

DOI: 10.1007/s007050050431
PubMed: 9856082
PubMed Central: 7086726

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PMC:7086726

Le document en format XML

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<front><div type="abstract" xml:lang="en">We analyzed the characteristics of seven monoclonal antibodies (mAbs) raised against purified HE (hemagglutinin-esterase) glycoprotein of the murine coronavirus DVIM (diarrhea virus of infant mice). Immunocrossreaction of these mAbs with JHM and/or MHV-S suggest that antigenic epitopes of HE of DVIM are similar to those of JHM and/or MHV-S. Four mAbs (1b4, 3a28, 4c19, 10b7), designated as group A mAbs, strongly inhibited both HA and AE activities. On the other hand, three mAbs (5a3, 6a6, 13a4), referred to as group B, had a comparatively weak HA inhibition activity. These results indicate that the antigenic epitopes of this glycoprotein can be classified into at least two groups and that the functional sites of HA and AE activities are similar but not identical. Neutralizing activity was shown in group A mAbs exclusively, suggesting that the ratio of HA and/or AE activities may play important roles in the cell fusion activity of DVIM-infected cells.</div>
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Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies

Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies

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Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki