Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies
Identifieur interne : 000188 ( Pmc/Curation ); précédent : 000187; suivant : 000189Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies
Auteurs : H. Kasai ; E. Morita ; K. Hatakeyama ; K. SugiyamaSource :
- Archives of Virology [ 0304-8608 ] ; 1998.
Abstract
We analyzed the characteristics of seven monoclonal antibodies (mAbs) raised against purified HE (hemagglutinin-esterase) glycoprotein of the murine coronavirus DVIM (diarrhea virus of infant mice). Immunocrossreaction of these mAbs with JHM and/or MHV-S suggest that antigenic epitopes of HE of DVIM are similar to those of JHM and/or MHV-S. Four mAbs (1b4, 3Aff28, 4c19, 10b7), designated as group A mAbs, strongly inhibited both HA and AE activities. On the other hand, three mAbs (5Aff3, 6Aff6, 13Aff4), referred to as group B, had a comparatively weak HA inhibition activity. These results indicate that the antigenic epitopes of this glycoprotein can be classified into at least two groups and that the functional sites of HA and AE activities are similar but not identical. Neutralizing activity was shown in group A mAbs exclusively, suggesting that the ratio of HA and/or AE activities may play important roles in the cell fusion activity of DVIM-infected cells.
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DOI: 10.1007/s007050050431
PubMed: 9856082
PubMed Central: 7086726
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<front><div type="abstract" xml:lang="en"><title>Summary</title>
<p>We analyzed the characteristics of seven monoclonal antibodies (mAbs) raised against purified HE (hemagglutinin-esterase) glycoprotein of the murine coronavirus DVIM (diarrhea virus of infant mice). Immunocrossreaction of these mAbs with JHM and/or MHV-S suggest that antigenic epitopes of HE of DVIM are similar to those of JHM and/or MHV-S. Four mAbs (1b4, 3Aff28, 4c19, 10b7), designated as group A mAbs, strongly inhibited both HA and AE activities. On the other hand, three mAbs (5Aff3, 6Aff6, 13Aff4), referred to as group B, had a comparatively weak HA inhibition activity. These results indicate that the antigenic epitopes of this glycoprotein can be classified into at least two groups and that the functional sites of HA and AE activities are similar but not identical. Neutralizing activity was shown in group A mAbs exclusively, suggesting that the ratio of HA and/or AE activities may play important roles in the cell fusion activity of DVIM-infected cells.</p>
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<title-group><article-title>Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies</article-title>
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<contrib-group><contrib contrib-type="author"><name><surname>Kasai</surname>
<given-names>H.</given-names>
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<contrib contrib-type="author"><name><surname>Morita</surname>
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<contrib contrib-type="author"><name><surname>Hatakeyama</surname>
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<institution-id institution-id-type="ISNI">0000000106736172</institution-id>
<institution>Department of Biology, Faculty of Science, Hirosaki University, Hirosaki, Japan,</institution>
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Japan</aff>
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<pub-date pub-type="epub"><day>8</day>
<month>4</month>
<year>2014</year>
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<volume>143</volume>
<issue>10</issue>
<fpage>1941</fpage>
<lpage>1948</lpage>
<permissions><copyright-statement>© Springer-Verlag 1998</copyright-statement>
<license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p>
</license>
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<abstract id="Abs1"><title>Summary</title>
<p>We analyzed the characteristics of seven monoclonal antibodies (mAbs) raised against purified HE (hemagglutinin-esterase) glycoprotein of the murine coronavirus DVIM (diarrhea virus of infant mice). Immunocrossreaction of these mAbs with JHM and/or MHV-S suggest that antigenic epitopes of HE of DVIM are similar to those of JHM and/or MHV-S. Four mAbs (1b4, 3Aff28, 4c19, 10b7), designated as group A mAbs, strongly inhibited both HA and AE activities. On the other hand, three mAbs (5Aff3, 6Aff6, 13Aff4), referred to as group B, had a comparatively weak HA inhibition activity. These results indicate that the antigenic epitopes of this glycoprotein can be classified into at least two groups and that the functional sites of HA and AE activities are similar but not identical. Neutralizing activity was shown in group A mAbs exclusively, suggesting that the ratio of HA and/or AE activities may play important roles in the cell fusion activity of DVIM-infected cells.</p>
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