CovidV1, PubMed, Checkpoint, bibRecord, 000611

Infectious bronchitis corona virus establishes productive infection in avian macrophages interfering with selected antimicrobial functions.

Identifieur interne : 000611 ( PubMed/Checkpoint ); précédent : 000610; suivant : 000612

Infectious bronchitis corona virus establishes productive infection in avian macrophages interfering with selected antimicrobial functions.

Auteurs : Aruna Amarasinghe [Canada] ; Mohamed Sarjoon Abdul-Cader [Canada] ; Sadiya Nazir [Canada] ; Upasama De Silva Senapathi [Canada] ; Frank Van Der Meer [Canada] ; Susan Catherine Cork [Canada] ; Susantha Gomis [Canada] ; Mohamed Faizal Abdul-Careem [Canada]

Source :

PloS one [ 1932-6203 ] ; 2017.

RBID : pubmed:28763472

Descripteurs français

KwdFr :
- Animaux, Génome viral, Infections à coronavirus (immunologie), Macrophages (immunologie), Macrophages (virologie), Maladies de la volaille (immunologie), Maladies de la volaille (virologie), Microscopie de fluorescence, Monoxyde d'azote (), Poulets, Poumon (anatomopathologie), Trachée (anatomopathologie), Virus de la bronchite infectieuse.
MESH :
- anatomopathologie : Poumon, Trachée.
- immunologie : Infections à coronavirus, Macrophages, Maladies de la volaille.
- virologie : Macrophages, Maladies de la volaille.
- Animaux, Génome viral, Microscopie de fluorescence, Monoxyde d'azote, Poulets, Virus de la bronchite infectieuse.

English descriptors

KwdEn :
- Animals, Chickens, Coronavirus Infections (immunology), Genome, Viral, Infectious bronchitis virus, Lung (pathology), Macrophages (immunology), Macrophages (virology), Microscopy, Fluorescence, Nitric Oxide (chemistry), Poultry Diseases (immunology), Poultry Diseases (virology), Trachea (pathology).
MESH :
- chemical , chemistry : Nitric Oxide.
- immunology : Coronavirus Infections, Macrophages, Poultry Diseases.
- pathology : Lung, Trachea.
- virology : Macrophages, Poultry Diseases.
- Animals, Chickens, Genome, Viral, Infectious bronchitis virus, Microscopy, Fluorescence.

Abstract

Infectious bronchitis virus (IBV) causes respiratory disease leading to loss of egg and meat production in chickens. Although it is known that macrophage numbers are elevated in the respiratory tract of IBV infected chickens, the role played by macrophages in IBV infection, particularly as a target cell for viral replication, is unknown. In this study, first, we investigated the ability of IBV to establish productive replication in macrophages in lungs and trachea in vivo and in macrophage cell cultures in vitro using two pathogenic IBV strains. Using a double immunofluorescent technique, we observed that both IBV Massachusetts-type 41 (M41) and Connecticut A5968 (Conn A5968) strains replicate in avian macrophages at a low level in vivo. This in vivo observation was substantiated by demonstrating IBV antigens in macrophages following in vitro IBV infection. Further, IBV productive infection in macrophages was confirmed by demonstrating corona viral particles in macrophages and IBV ribonucleic acid (RNA) in culture supernatants. Evaluation of the functions of macrophages following infection of macrophages with IBV M41 and Conn A5968 strains revealed that the production of antimicrobial molecule, nitric oxide (NO) is inhibited. It was also noted that replication of IBV M41 and Conn A5968 strains in macrophages does not interfere with the induction of type 1 IFN activity by macrophages. In conclusion, both M41 and Con A5968 IBV strains infect macrophages in vivo and in vitro resulting productive replications. During the replication of IBV in macrophages, their ability to produce NO can be affected without affecting the ability to induce type 1 IFN activity. Further studies are warranted to uncover the significance of macrophage infection of IBV in the pathogenesis of IBV infection in chickens.

DOI: 10.1371/journal.pone.0181801
PubMed: 28763472

Affiliations:

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Le document en format XML

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<term>Coronavirus Infections (immunology)</term>
<term>Genome, Viral</term>
<term>Infectious bronchitis virus</term>
<term>Lung (pathology)</term>
<term>Macrophages (immunology)</term>
<term>Macrophages (virology)</term>
<term>Microscopy, Fluorescence</term>
<term>Nitric Oxide (chemistry)</term>
<term>Poultry Diseases (immunology)</term>
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<term>Maladies de la volaille (immunologie)</term>
<term>Maladies de la volaille (virologie)</term>
<term>Microscopie de fluorescence</term>
<term>Monoxyde d'azote ()</term>
<term>Poulets</term>
<term>Poumon (anatomopathologie)</term>
<term>Trachée (anatomopathologie)</term>
<term>Virus de la bronchite infectieuse</term>
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<term>Trachée</term>
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<front><div type="abstract" xml:lang="en">Infectious bronchitis virus (IBV) causes respiratory disease leading to loss of egg and meat production in chickens. Although it is known that macrophage numbers are elevated in the respiratory tract of IBV infected chickens, the role played by macrophages in IBV infection, particularly as a target cell for viral replication, is unknown. In this study, first, we investigated the ability of IBV to establish productive replication in macrophages in lungs and trachea in vivo and in macrophage cell cultures in vitro using two pathogenic IBV strains. Using a double immunofluorescent technique, we observed that both IBV Massachusetts-type 41 (M41) and Connecticut A5968 (Conn A5968) strains replicate in avian macrophages at a low level in vivo. This in vivo observation was substantiated by demonstrating IBV antigens in macrophages following in vitro IBV infection. Further, IBV productive infection in macrophages was confirmed by demonstrating corona viral particles in macrophages and IBV ribonucleic acid (RNA) in culture supernatants. Evaluation of the functions of macrophages following infection of macrophages with IBV M41 and Conn A5968 strains revealed that the production of antimicrobial molecule, nitric oxide (NO) is inhibited. It was also noted that replication of IBV M41 and Conn A5968 strains in macrophages does not interfere with the induction of type 1 IFN activity by macrophages. In conclusion, both M41 and Con A5968 IBV strains infect macrophages in vivo and in vitro resulting productive replications. During the replication of IBV in macrophages, their ability to produce NO can be affected without affecting the ability to induce type 1 IFN activity. Further studies are warranted to uncover the significance of macrophage infection of IBV in the pathogenesis of IBV infection in chickens.</div>
</front>
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<DateCompleted><Year>2017</Year>
<Month>10</Month>
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<Abstract><AbstractText>Infectious bronchitis virus (IBV) causes respiratory disease leading to loss of egg and meat production in chickens. Although it is known that macrophage numbers are elevated in the respiratory tract of IBV infected chickens, the role played by macrophages in IBV infection, particularly as a target cell for viral replication, is unknown. In this study, first, we investigated the ability of IBV to establish productive replication in macrophages in lungs and trachea in vivo and in macrophage cell cultures in vitro using two pathogenic IBV strains. Using a double immunofluorescent technique, we observed that both IBV Massachusetts-type 41 (M41) and Connecticut A5968 (Conn A5968) strains replicate in avian macrophages at a low level in vivo. This in vivo observation was substantiated by demonstrating IBV antigens in macrophages following in vitro IBV infection. Further, IBV productive infection in macrophages was confirmed by demonstrating corona viral particles in macrophages and IBV ribonucleic acid (RNA) in culture supernatants. Evaluation of the functions of macrophages following infection of macrophages with IBV M41 and Conn A5968 strains revealed that the production of antimicrobial molecule, nitric oxide (NO) is inhibited. It was also noted that replication of IBV M41 and Conn A5968 strains in macrophages does not interfere with the induction of type 1 IFN activity by macrophages. In conclusion, both M41 and Con A5968 IBV strains infect macrophages in vivo and in vitro resulting productive replications. During the replication of IBV in macrophages, their ability to produce NO can be affected without affecting the ability to induce type 1 IFN activity. Further studies are warranted to uncover the significance of macrophage infection of IBV in the pathogenesis of IBV infection in chickens.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Amarasinghe</LastName>
<ForeName>Aruna</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C53, Calgary, Alberta, Canada.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Abdul-Cader</LastName>
<ForeName>Mohamed Sarjoon</ForeName>
<Initials>MS</Initials>
<AffiliationInfo><Affiliation>Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C53, Calgary, Alberta, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nazir</LastName>
<ForeName>Sadiya</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C53, Calgary, Alberta, Canada.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>De Silva Senapathi</LastName>
<ForeName>Upasama</ForeName>
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</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>van der Meer</LastName>
<ForeName>Frank</ForeName>
<Initials>F</Initials>
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</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Cork</LastName>
<ForeName>Susan Catherine</ForeName>
<Initials>SC</Initials>
<AffiliationInfo><Affiliation>Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C53, Calgary, Alberta, Canada.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Gomis</LastName>
<ForeName>Susantha</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatoon, Canada.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Abdul-Careem</LastName>
<ForeName>Mohamed Faizal</ForeName>
<Initials>MF</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0002-9974-7600</Identifier>
<AffiliationInfo><Affiliation>Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Health Research Innovation Center 2C53, Calgary, Alberta, Canada.</Affiliation>
</AffiliationInfo>
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Infectious bronchitis corona virus establishes productive infection in avian macrophages interfering with selected antimicrobial functions.

Infectious bronchitis corona virus establishes productive infection in avian macrophages interfering with selected antimicrobial functions.

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