Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
Identifieur interne : 000C82 ( Ncbi/Curation ); précédent : 000C81; suivant : 000C83Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
Auteurs : Regina Konrad ; Ute Eberle [Allemagne] ; Alexandra Dangel ; Bianca Treis [Allemagne] ; Anja Berger [Allemagne] ; Katja Bengs [Allemagne] ; Volker Fingerle [Allemagne] ; Bernhard Liebl [Allemagne] ; Nikolaus Ackermann [Allemagne] ; Andreas Sing [Allemagne]Source :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2020.
Abstract
The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
DOI: 10.2807/1560-7917.ES.2020.25.9.2000173
PubMed: 32156330
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000139
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000139
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000196
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000C82
Links to Exploration step
pubmed:32156330Curation
No country items
Regina Konrad<affiliation><nlm:affiliation>These authors contributed equally to this work.</nlm:affiliation>
<wicri:noCountry code="no comma">These authors contributed equally to this work.</wicri:noCountry>
</affiliation>
<affiliation><nlm:affiliation>These authors contributed equally to this work.</nlm:affiliation>
<wicri:noCountry code="no comma">These authors contributed equally to this work.</wicri:noCountry>
</affiliation>
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.</title>
<author><name sortKey="Konrad, Regina" sort="Konrad, Regina" uniqKey="Konrad R" first="Regina" last="Konrad">Regina Konrad</name>
<affiliation><nlm:affiliation>These authors contributed equally to this work.</nlm:affiliation>
<wicri:noCountry code="no comma">These authors contributed equally to this work.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Eberle, Ute" sort="Eberle, Ute" uniqKey="Eberle U" first="Ute" last="Eberle">Ute Eberle</name>
<affiliation wicri:level="1"><nlm:affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dangel, Alexandra" sort="Dangel, Alexandra" uniqKey="Dangel A" first="Alexandra" last="Dangel">Alexandra Dangel</name>
<affiliation><nlm:affiliation>These authors contributed equally to this work.</nlm:affiliation>
<wicri:noCountry code="no comma">These authors contributed equally to this work.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Treis, Bianca" sort="Treis, Bianca" uniqKey="Treis B" first="Bianca" last="Treis">Bianca Treis</name>
<affiliation wicri:level="1"><nlm:affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Berger, Anja" sort="Berger, Anja" uniqKey="Berger A" first="Anja" last="Berger">Anja Berger</name>
<affiliation wicri:level="1"><nlm:affiliation>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bengs, Katja" sort="Bengs, Katja" uniqKey="Bengs K" first="Katja" last="Bengs">Katja Bengs</name>
<affiliation wicri:level="1"><nlm:affiliation>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fingerle, Volker" sort="Fingerle, Volker" uniqKey="Fingerle V" first="Volker" last="Fingerle">Volker Fingerle</name>
<affiliation wicri:level="1"><nlm:affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Liebl, Bernhard" sort="Liebl, Bernhard" uniqKey="Liebl B" first="Bernhard" last="Liebl">Bernhard Liebl</name>
<affiliation wicri:level="3"><nlm:affiliation>Ludwig Maximilians-Universität, Munich, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea>
<placeName><region type="land" nuts="1">Bavière</region>
<region type="district" nuts="2">District de Haute-Bavière</region>
<settlement type="city">Munich</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ackermann, Nikolaus" sort="Ackermann, Nikolaus" uniqKey="Ackermann N" first="Nikolaus" last="Ackermann">Nikolaus Ackermann</name>
<affiliation wicri:level="1"><nlm:affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sing, Andreas" sort="Sing, Andreas" uniqKey="Sing A" first="Andreas" last="Sing">Andreas Sing</name>
<affiliation wicri:level="3"><nlm:affiliation>Ludwig Maximilians-Universität, Munich, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea>
<placeName><region type="land" nuts="1">Bavière</region>
<region type="district" nuts="2">District de Haute-Bavière</region>
<settlement type="city">Munich</settlement>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2020">2020</date>
<idno type="RBID">pubmed:32156330</idno>
<idno type="pmid">32156330</idno>
<idno type="doi">10.2807/1560-7917.ES.2020.25.9.2000173</idno>
<idno type="wicri:Area/PubMed/Corpus">000139</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000139</idno>
<idno type="wicri:Area/PubMed/Curation">000139</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000139</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000196</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000196</idno>
<idno type="wicri:Area/Ncbi/Merge">000C82</idno>
<idno type="wicri:Area/Ncbi/Curation">000C82</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.</title>
<author><name sortKey="Konrad, Regina" sort="Konrad, Regina" uniqKey="Konrad R" first="Regina" last="Konrad">Regina Konrad</name>
<affiliation><nlm:affiliation>These authors contributed equally to this work.</nlm:affiliation>
<wicri:noCountry code="no comma">These authors contributed equally to this work.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Eberle, Ute" sort="Eberle, Ute" uniqKey="Eberle U" first="Ute" last="Eberle">Ute Eberle</name>
<affiliation wicri:level="1"><nlm:affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dangel, Alexandra" sort="Dangel, Alexandra" uniqKey="Dangel A" first="Alexandra" last="Dangel">Alexandra Dangel</name>
<affiliation><nlm:affiliation>These authors contributed equally to this work.</nlm:affiliation>
<wicri:noCountry code="no comma">These authors contributed equally to this work.</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Treis, Bianca" sort="Treis, Bianca" uniqKey="Treis B" first="Bianca" last="Treis">Bianca Treis</name>
<affiliation wicri:level="1"><nlm:affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Berger, Anja" sort="Berger, Anja" uniqKey="Berger A" first="Anja" last="Berger">Anja Berger</name>
<affiliation wicri:level="1"><nlm:affiliation>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bengs, Katja" sort="Bengs, Katja" uniqKey="Bengs K" first="Katja" last="Bengs">Katja Bengs</name>
<affiliation wicri:level="1"><nlm:affiliation>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Fingerle, Volker" sort="Fingerle, Volker" uniqKey="Fingerle V" first="Volker" last="Fingerle">Volker Fingerle</name>
<affiliation wicri:level="1"><nlm:affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Liebl, Bernhard" sort="Liebl, Bernhard" uniqKey="Liebl B" first="Bernhard" last="Liebl">Bernhard Liebl</name>
<affiliation wicri:level="3"><nlm:affiliation>Ludwig Maximilians-Universität, Munich, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea>
<placeName><region type="land" nuts="1">Bavière</region>
<region type="district" nuts="2">District de Haute-Bavière</region>
<settlement type="city">Munich</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ackermann, Nikolaus" sort="Ackermann, Nikolaus" uniqKey="Ackermann N" first="Nikolaus" last="Ackermann">Nikolaus Ackermann</name>
<affiliation wicri:level="1"><nlm:affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
<wicri:noRegion>Oberschleißheim</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sing, Andreas" sort="Sing, Andreas" uniqKey="Sing A" first="Andreas" last="Sing">Andreas Sing</name>
<affiliation wicri:level="3"><nlm:affiliation>Ludwig Maximilians-Universität, Munich, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea>
<placeName><region type="land" nuts="1">Bavière</region>
<region type="district" nuts="2">District de Haute-Bavière</region>
<settlement type="city">Munich</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</title>
<idno type="eISSN">1560-7917</idno>
<imprint><date when="2020" type="published">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C82 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000C82 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV1 |flux= Ncbi |étape= Curation |type= RBID |clé= pubmed:32156330 |texte= Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:32156330" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a CovidV1
This area was generated with Dilib version V0.6.33. |