Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
Identifieur interne : 000139 ( PubMed/Curation ); précédent : 000138; suivant : 000140Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
Auteurs : Regina Konrad ; Ute Eberle [Allemagne] ; Alexandra Dangel ; Bianca Treis [Allemagne] ; Anja Berger [Allemagne] ; Katja Bengs [Allemagne] ; Volker Fingerle [Allemagne] ; Bernhard Liebl [Allemagne] ; Nikolaus Ackermann [Allemagne] ; Andreas Sing [Allemagne]Source :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2020.
Abstract
The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
DOI: 10.2807/1560-7917.ES.2020.25.9.2000173
PubMed: 32156330
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Regina Konrad<affiliation><nlm:affiliation>These authors contributed equally to this work.</nlm:affiliation>
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<wicri:noCountry code="no comma">These authors contributed equally to this work.</wicri:noCountry>
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<front><div type="abstract" xml:lang="en">The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.</div>
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