Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus
Identifieur interne : 000139 ( Istex/Curation ); précédent : 000138; suivant : 000140Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus
Auteurs : Samir Mounir [Canada] ; Pierre J. Talbot [Canada]Source :
- Virology [ 0042-6822 ] ; 1993.
Abstract
Abstract: Nucleotide sequences between the spike (S) and membrane (M) protein genes of the OC43 strain of human coronavirus were obtained from POP-amplified vital mRNAs. Sequence analysis of this region revealed the presence of two ORFs encoding proteins of 12.9 and 9.5 kDa. These two proteins were identified as putatively nonstructural (ns) due to their homology to the corresponding BCV ns gene products. Northern blot analysis indicated that each of these two genes was present on a separate mRNA (5 and 5-1, respectively). In vitro translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated downstream of the 12.9-kDa ORF, although immunofluorescence studies did confirm its presence in infected cells. Sequence analysis showed that a large portion of the 3′-end of the leader sequence is present within the viral genome, upstream of the 12.9-kDa ORF. In addition, two ORFs encoding potential 4.9- and 4.8-kDa ns proteins in BCV are absent in HCV-OC43, although a corresponding mRNA 4 was found at a very low level. These results demonstrate that these two putative ns proteins are not essential for virus replication, at least in HRT-18 cells.
Url:
DOI: 10.1006/viro.1993.1043
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus</title><author><name sortKey="Mounir, Samir" sort="Mounir, Samir" uniqKey="Mounir S" first="Samir" last="Mounir">Samir Mounir</name><affiliation wicri:level="1"><mods:affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</mods:affiliation><country>Canada</country><wicri:regionArea>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec</wicri:regionArea></affiliation></author><author><name sortKey="Talbot, Pierre J" sort="Talbot, Pierre J" uniqKey="Talbot P" first="Pierre J." last="Talbot">Pierre J. Talbot</name><affiliation wicri:level="1"><mods:affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</mods:affiliation><country>Canada</country><wicri:regionArea>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:12DAF5C1C70FB276018BB2F07E0B71E18F2D3B93</idno><date when="1993" year="1993">1993</date><idno type="doi">10.1006/viro.1993.1043</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-LGGNT4CC-9/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000159</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000159</idno><idno type="wicri:Area/Istex/Curation">000139</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus</title><author><name sortKey="Mounir, Samir" sort="Mounir, Samir" uniqKey="Mounir S" first="Samir" last="Mounir">Samir Mounir</name><affiliation wicri:level="1"><mods:affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</mods:affiliation><country>Canada</country><wicri:regionArea>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec</wicri:regionArea></affiliation></author><author><name sortKey="Talbot, Pierre J" sort="Talbot, Pierre J" uniqKey="Talbot P" first="Pierre J." last="Talbot">Pierre J. Talbot</name><affiliation wicri:level="1"><mods:affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</mods:affiliation><country>Canada</country><wicri:regionArea>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">192</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="355">355</biblScope><biblScope unit="page" to="360">360</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Nucleotide sequences between the spike (S) and membrane (M) protein genes of the OC43 strain of human coronavirus were obtained from POP-amplified vital mRNAs. Sequence analysis of this region revealed the presence of two ORFs encoding proteins of 12.9 and 9.5 kDa. These two proteins were identified as putatively nonstructural (ns) due to their homology to the corresponding BCV ns gene products. Northern blot analysis indicated that each of these two genes was present on a separate mRNA (5 and 5-1, respectively). In vitro translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated downstream of the 12.9-kDa ORF, although immunofluorescence studies did confirm its presence in infected cells. Sequence analysis showed that a large portion of the 3′-end of the leader sequence is present within the viral genome, upstream of the 12.9-kDa ORF. In addition, two ORFs encoding potential 4.9- and 4.8-kDa ns proteins in BCV are absent in HCV-OC43, although a corresponding mRNA 4 was found at a very low level. These results demonstrate that these two putative ns proteins are not essential for virus replication, at least in HRT-18 cells.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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