Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus
Identifieur interne : 000159 ( Istex/Corpus ); précédent : 000158; suivant : 000160Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus
Auteurs : Samir Mounir ; Pierre J. TalbotSource :
- Virology [ 0042-6822 ] ; 1993.
Abstract
Abstract: Nucleotide sequences between the spike (S) and membrane (M) protein genes of the OC43 strain of human coronavirus were obtained from POP-amplified vital mRNAs. Sequence analysis of this region revealed the presence of two ORFs encoding proteins of 12.9 and 9.5 kDa. These two proteins were identified as putatively nonstructural (ns) due to their homology to the corresponding BCV ns gene products. Northern blot analysis indicated that each of these two genes was present on a separate mRNA (5 and 5-1, respectively). In vitro translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated downstream of the 12.9-kDa ORF, although immunofluorescence studies did confirm its presence in infected cells. Sequence analysis showed that a large portion of the 3′-end of the leader sequence is present within the viral genome, upstream of the 12.9-kDa ORF. In addition, two ORFs encoding potential 4.9- and 4.8-kDa ns proteins in BCV are absent in HCV-OC43, although a corresponding mRNA 4 was found at a very low level. These results demonstrate that these two putative ns proteins are not essential for virus replication, at least in HRT-18 cells.
Url:
DOI: 10.1006/viro.1993.1043
Links to Exploration step
ISTEX:12DAF5C1C70FB276018BB2F07E0B71E18F2D3B93Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus</title><author><name sortKey="Mounir, Samir" sort="Mounir, Samir" uniqKey="Mounir S" first="Samir" last="Mounir">Samir Mounir</name><affiliation><mods:affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</mods:affiliation></affiliation></author><author><name sortKey="Talbot, Pierre J" sort="Talbot, Pierre J" uniqKey="Talbot P" first="Pierre J." last="Talbot">Pierre J. Talbot</name><affiliation><mods:affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:12DAF5C1C70FB276018BB2F07E0B71E18F2D3B93</idno><date when="1993" year="1993">1993</date><idno type="doi">10.1006/viro.1993.1043</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-LGGNT4CC-9/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000159</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000159</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus</title><author><name sortKey="Mounir, Samir" sort="Mounir, Samir" uniqKey="Mounir S" first="Samir" last="Mounir">Samir Mounir</name><affiliation><mods:affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</mods:affiliation></affiliation></author><author><name sortKey="Talbot, Pierre J" sort="Talbot, Pierre J" uniqKey="Talbot P" first="Pierre J." last="Talbot">Pierre J. Talbot</name><affiliation><mods:affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">192</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="355">355</biblScope><biblScope unit="page" to="360">360</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Nucleotide sequences between the spike (S) and membrane (M) protein genes of the OC43 strain of human coronavirus were obtained from POP-amplified vital mRNAs. Sequence analysis of this region revealed the presence of two ORFs encoding proteins of 12.9 and 9.5 kDa. These two proteins were identified as putatively nonstructural (ns) due to their homology to the corresponding BCV ns gene products. Northern blot analysis indicated that each of these two genes was present on a separate mRNA (5 and 5-1, respectively). In vitro translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated downstream of the 12.9-kDa ORF, although immunofluorescence studies did confirm its presence in infected cells. Sequence analysis showed that a large portion of the 3′-end of the leader sequence is present within the viral genome, upstream of the 12.9-kDa ORF. In addition, two ORFs encoding potential 4.9- and 4.8-kDa ns proteins in BCV are absent in HCV-OC43, although a corresponding mRNA 4 was found at a very low level. These results demonstrate that these two putative ns proteins are not essential for virus replication, at least in HRT-18 cells.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Samir Mounir</name><affiliations><json:string>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</json:string></affiliations></json:item><json:item><name>Pierre J. Talbot</name><affiliations><json:string>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-LGGNT4CC-9</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Short communication</json:string></originalGenre><abstract>Abstract: Nucleotide sequences between the spike (S) and membrane (M) protein genes of the OC43 strain of human coronavirus were obtained from POP-amplified vital mRNAs. Sequence analysis of this region revealed the presence of two ORFs encoding proteins of 12.9 and 9.5 kDa. These two proteins were identified as putatively nonstructural (ns) due to their homology to the corresponding BCV ns gene products. Northern blot analysis indicated that each of these two genes was present on a separate mRNA (5 and 5-1, respectively). In vitro translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated downstream of the 12.9-kDa ORF, although immunofluorescence studies did confirm its presence in infected cells. Sequence analysis showed that a large portion of the 3′-end of the leader sequence is present within the viral genome, upstream of the 12.9-kDa ORF. In addition, two ORFs encoding potential 4.9- and 4.8-kDa ns proteins in BCV are absent in HCV-OC43, although a corresponding mRNA 4 was found at a very low level. These results demonstrate that these two putative ns proteins are not essential for virus replication, at least in HRT-18 cells.</abstract><qualityIndicators><refBibsNative>false</refBibsNative><abstractWordCount>191</abstractWordCount><abstractCharCount>1221</abstractCharCount><keywordCount>0</keywordCount><score>4.342</score><pdfWordCount>0</pdfWordCount><pdfCharCount>0</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>6</pdfPageCount><pdfPageSize>610 x 803 pts</pdfPageSize></qualityIndicators><title>Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus</title><pmid><json:string>8517026</json:string></pmid><pii><json:string>S0042-6822(83)71043-3</json:string></pii><genre><json:string>brief-communication</json:string></genre><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>1993</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0185-7</json:string></pii><volume>192</volume><issue>1</issue><pages><first>355</first><last>360</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-LGGNT4CC-9</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - microbiologie</json:string></inist></categories><publicationDate>1993</publicationDate><copyrightDate>1993</copyrightDate><doi><json:string>10.1006/viro.1993.1043</json:string></doi><id>12DAF5C1C70FB276018BB2F07E0B71E18F2D3B93</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LGGNT4CC-9/fulltext.pdf</uri></json:item><json:item><extension>ocr</extension><original>false</original><mimetype>text/ocr</mimetype><quality><totalToken>4585</totalToken><correct>4029</correct><rate>79.61379622757508</rate><misspelled>550</misspelled></quality><langDetect><reliable>true</reliable><languages><json:item><score>969</score><code>en</code><name>ENGLISH</name><percent>92</percent></json:item></languages></langDetect><uri>https://api.istex.fr/ark:/67375/6H6-LGGNT4CC-9/fulltext.ocr</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LGGNT4CC-9/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-LGGNT4CC-9/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©1993 Academic Press</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>1993</date></publicationStmt><notesStmt><note type="brief-communication" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Short Communications</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus</title><author xml:id="author-0000"><persName><forename type="first">Samir</forename><surname>Mounir</surname></persName><affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Pierre J.</forename><surname>Talbot</surname></persName><affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</affiliation></author><idno type="istex">12DAF5C1C70FB276018BB2F07E0B71E18F2D3B93</idno><idno type="ark">ark:/67375/6H6-LGGNT4CC-9</idno><idno type="DOI">10.1006/viro.1993.1043</idno><idno type="PII">S0042-6822(83)71043-3</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0185-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993"></date><biblScope unit="volume">192</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="355">355</biblScope><biblScope unit="page" to="360">360</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1993</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Nucleotide sequences between the spike (S) and membrane (M) protein genes of the OC43 strain of human coronavirus were obtained from POP-amplified vital mRNAs. Sequence analysis of this region revealed the presence of two ORFs encoding proteins of 12.9 and 9.5 kDa. These two proteins were identified as putatively nonstructural (ns) due to their homology to the corresponding BCV ns gene products. Northern blot analysis indicated that each of these two genes was present on a separate mRNA (5 and 5-1, respectively). In vitro translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated downstream of the 12.9-kDa ORF, although immunofluorescence studies did confirm its presence in infected cells. Sequence analysis showed that a large portion of the 3′-end of the leader sequence is present within the viral genome, upstream of the 12.9-kDa ORF. In addition, two ORFs encoding potential 4.9- and 4.8-kDa ns proteins in BCV are absent in HCV-OC43, although a corresponding mRNA 4 was found at a very low level. These results demonstrate that these two putative ns proteins are not essential for virus replication, at least in HRT-18 cells.</p></abstract></profileDesc><revisionDesc><change when="1993">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LGGNT4CC-9/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="sco" xml:lang="en"><item-info><jid>YVIRO</jid><aid>71043</aid><ce:pii>S0042-6822(83)71043-3</ce:pii><ce:doi>10.1006/viro.1993.1043</ce:doi><ce:copyright type="full-transfer" year="1993">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Short Communications</ce:textfn></ce:dochead><ce:title>Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus</ce:title><ce:author-group><ce:author><ce:given-name>Samir</ce:given-name><ce:surname>Mounir</ce:surname></ce:author><ce:author><ce:given-name>Pierre J.</ce:given-name><ce:surname>Talbot</ce:surname></ce:author><ce:affiliation><ce:textfn>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</ce:textfn></ce:affiliation></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Nucleotide sequences between the spike (S) and membrane (M) protein genes of the OC43 strain of human coronavirus were obtained from POP-amplified vital mRNAs. Sequence analysis of this region revealed the presence of two ORFs encoding proteins of 12.9 and 9.5 kDa. These two proteins were identified as putatively nonstructural (ns) due to their homology to the corresponding BCV ns gene products. Northern blot analysis indicated that each of these two genes was present on a separate mRNA (5 and 5-1, respectively). <ce:italic>In vitro</ce:italic> translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated downstream of the 12.9-kDa ORF, although immunofluorescence studies did confirm its presence in infected cells. Sequence analysis showed that a large portion of the 3′-end of the leader sequence is present within the viral genome, upstream of the 12.9-kDa ORF. In addition, two ORFs encoding potential 4.9- and 4.8-kDa ns proteins in BCV are absent in HCV-OC43, although a corresponding mRNA 4 was found at a very low level. These results demonstrate that these two putative ns proteins are not essential for virus replication, at least in HRT-18 cells.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus</title></titleInfo><name type="personal"><namePart type="given">Samir</namePart><namePart type="family">Mounir</namePart><affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pierre J.</namePart><namePart type="family">Talbot</namePart><affiliation>Virology Research Center, Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, Québec, Canada H7N 4Z3</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="Short communication" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Nucleotide sequences between the spike (S) and membrane (M) protein genes of the OC43 strain of human coronavirus were obtained from POP-amplified vital mRNAs. Sequence analysis of this region revealed the presence of two ORFs encoding proteins of 12.9 and 9.5 kDa. These two proteins were identified as putatively nonstructural (ns) due to their homology to the corresponding BCV ns gene products. Northern blot analysis indicated that each of these two genes was present on a separate mRNA (5 and 5-1, respectively). In vitro translation analyses demonstrated that the HCV-OC43 9.5-kDa protein, like its BCV counterpart, is poorly translated when situated downstream of the 12.9-kDa ORF, although immunofluorescence studies did confirm its presence in infected cells. Sequence analysis showed that a large portion of the 3′-end of the leader sequence is present within the viral genome, upstream of the 12.9-kDa ORF. In addition, two ORFs encoding potential 4.9- and 4.8-kDa ns proteins in BCV are absent in HCV-OC43, although a corresponding mRNA 4 was found at a very low level. These results demonstrate that these two putative ns proteins are not essential for virus replication, at least in HRT-18 cells.</abstract><note type="content">Section title: Short Communications</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0185-7</identifier><part><date>1993</date><detail type="volume"><number>192</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>400</end></extent><extent unit="pages"><start>355</start><end>360</end></extent></part></relatedItem><identifier type="istex">12DAF5C1C70FB276018BB2F07E0B71E18F2D3B93</identifier><identifier type="ark">ark:/67375/6H6-LGGNT4CC-9</identifier><identifier type="DOI">10.1006/viro.1993.1043</identifier><identifier type="PII">S0042-6822(83)71043-3</identifier><accessCondition type="use and reproduction" contentType="copyright">©1993 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1993</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LGGNT4CC-9/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000159 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000159 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:12DAF5C1C70FB276018BB2F07E0B71E18F2D3B93
|texte= Human Coronavirus OC43 RNA 4 Lacks Two Open Reading Frames Located Downstream of the S Gene of Bovine Coronavirus
}}
| This area was generated with Dilib version V0.6.33. |  |