Properties of a coronavirus isolated from a cow with epizootic diarrhea
Identifieur interne : 000273 ( Istex/Corpus ); précédent : 000272; suivant : 000274Properties of a coronavirus isolated from a cow with epizootic diarrhea
Auteurs : H. Akashi ; Y. Inaba ; Y. Miura ; S. Tokuhisa ; K. Sato ; K. SatodaSource :
- Veterinary Microbiology [ 0378-1135 ] ; 1980.
English descriptors
- Teeft :
- Adult cattle, American strain, American strain kakegawa, Angeles strain, Bovine, Bovine coronavirus, Bovine enterovirus, Buoyant density, Calf diarrhea, Calf diarrhea coronavirus, Chloroform, Coronavirus, Coronaviruses, Cytopathic changes, Electron microscopy, Epizootic diarrhea, Equal volume, Ether, Hemagglutination inhibition tests, Hemagglutinin activity, Inaba, Infectivity, Iudr, Kakegawa, Maintenance medium, Membrane filter, Neonatal calf diarrhea, Pore size, Publishing company, Reference viruses, Sato, Serum dilution, Takahashi, Trypsin, Virus suspension, Water bath, Water phase.
Abstract
Abstract: A coronavirus (Kakegawa isolate) isolated from a cow with epizootic diarrhea was grown in BEK-1 cells and examined for biophysical and biochemical properties. The Kakegawa isolate was able to replicate in the presence or absence of 5-iodo-2′-deoxy-uridine, indicating that its viral nucleic acid was RNA. It was highly sensitive to ether and chloroform, and moderately sensitive to trypsin and heat. It was, however, readily stabilized by treatment with cation at 50°C for 1 h. Its infectivity was slightly reduced at pH 3.0. The virus passed through a membrane filter of 200 nm pore size, but not through one of 100 nm pore size. The buoyant density of the virus was determined in a sucrose density gradient. The peak of infectivity and hemagglutinin activity was found at a density of 1.182. Neutralization and hemagglutination inhibition tests showed a close serological relationship between the Kakegawa isolate and the American strain of calf diarrhea coronavirus.
Url:
DOI: 10.1016/0378-1135(80)90025-5
Links to Exploration step
ISTEX:AB37DAE142E82FFA7C4E48D9ADFF9D5693FA9759Le document en format XML
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The Kakegawa isolate was able to replicate in the presence or absence of 5-iodo-2′-deoxy-uridine, indicating that its viral nucleic acid was RNA. It was highly sensitive to ether and chloroform, and moderately sensitive to trypsin and heat. It was, however, readily stabilized by treatment with cation at 50°C for 1 h. Its infectivity was slightly reduced at pH 3.0. The virus passed through a membrane filter of 200 nm pore size, but not through one of 100 nm pore size. The buoyant density of the virus was determined in a sucrose density gradient. The peak of infectivity and hemagglutinin activity was found at a density of 1.182. 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The buoyant density of the virus was determined in a sucrose density gradient. The peak of infectivity and hemagglutinin activity was found at a density of 1.182. Neutralization and hemagglutination inhibition tests showed a close serological relationship between the Kakegawa isolate and the American strain of calf diarrhea coronavirus.</abstract><qualityIndicators><refBibsNative>true</refBibsNative><abstractWordCount>153</abstractWordCount><abstractCharCount>979</abstractCharCount><keywordCount>0</keywordCount><score>7.111</score><pdfWordCount>3275</pdfWordCount><pdfCharCount>17841</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>12</pdfPageCount><pdfPageSize>461 x 677 pts</pdfPageSize></qualityIndicators><title>Properties of a coronavirus isolated from a cow with epizootic diarrhea</title><pii><json:string>0378-1135(80)90025-5</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Veterinary Microbiology</title><language><json:string>unknown</json:string></language><publicationDate>1980</publicationDate><issn><json:string>0378-1135</json:string></issn><pii><json:string>S0378-1135(00)X0189-7</json:string></pii><volume>5</volume><issue>4</issue><pages><first>265</first><last>276</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1980</json:string></date><geogName></geogName><orgName><json:string>Jeol Ltd.</json:string><json:string>University of Nebraska</json:string><json:string>Kakegawa American Kakegawa</json:string><json:string>SATODA National Institute of Animal Health, Tsukuba, Ibaraki</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Fractions</json:string></persName><placeName><json:string>Canada</json:string><json:string>Los Angeles</json:string><json:string>Japan</json:string><json:string>Denmark</json:string><json:string>Guinea</json:string><json:string>England</json:string><json:string>Belgium</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Acres et al., 1975</json:string><json:string>Inaba et al., 1976</json:string><json:string>Durham et al.,1979</json:string><json:string>Stair et al.,1972</json:string><json:string>Kurogl et al., 1976</json:string><json:string>Mebus et al.,1973</json:string><json:string>Pensaert and Callebaut, 1978</json:string><json:string>Sato et al. (1977a)</json:string><json:string>Bridger et ah, 1978b</json:string><json:string>Woode et al., 1978</json:string><json:string>Wellemans et al.,1977a, b</json:string><json:string>Sharpee et al., 1976</json:string><json:string>Bridget et al.,1978b</json:string><json:string>Bridger et al.,1978a</json:string><json:string>Sato et al. (1977b)</json:string><json:string>Hierholzer, 1976</json:string><json:string>Zygraich et al.,1975</json:string><json:string>Doughri et al.,1976</json:string><json:string>Takahashi et al., 1980</json:string><json:string>Inaba et al.,1976</json:string><json:string>Madin et al., 1956</json:string><json:string>McIntosh, 1974</json:string><json:string>Sharpee et al. (1976)</json:string><json:string>Homer et al.,1976</json:string><json:string>Deshmukh and P o m e r o y , 1974</json:string><json:string>Sharpee et al.,1976</json:string><json:string>Stott et al.,1977</json:string><json:string>Morin et al.,1976</json:string><json:string>Takahashi et al. 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The Kakegawa isolate was able to replicate in the presence or absence of 5-iodo-2′-deoxy-uridine, indicating that its viral nucleic acid was RNA. It was highly sensitive to ether and chloroform, and moderately sensitive to trypsin and heat. It was, however, readily stabilized by treatment with cation at 50°C for 1 h. Its infectivity was slightly reduced at pH 3.0. The virus passed through a membrane filter of 200 nm pore size, but not through one of 100 nm pore size. The buoyant density of the virus was determined in a sucrose density gradient. The peak of infectivity and hemagglutinin activity was found at a density of 1.182. Neutralization and hemagglutination inhibition tests showed a close serological relationship between the Kakegawa isolate and the American strain of calf diarrhea coronavirus.</p></abstract></profileDesc><revisionDesc><change when="1980">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-L4KZPZXF-5/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>VETMIC</jid><aid>80900255</aid><ce:pii>0378-1135(80)90025-5</ce:pii><ce:doi>10.1016/0378-1135(80)90025-5</ce:doi><ce:copyright type="unknown" year="1980"></ce:copyright></item-info><head><ce:title>Properties of a coronavirus isolated from a cow with epizootic diarrhea</ce:title><ce:author-group><ce:author><ce:given-name>H.</ce:given-name><ce:surname>Akashi</ce:surname></ce:author><ce:author><ce:given-name>Y.</ce:given-name><ce:surname>Inaba</ce:surname></ce:author><ce:author><ce:given-name>Y.</ce:given-name><ce:surname>Miura</ce:surname></ce:author><ce:author><ce:given-name>S.</ce:given-name><ce:surname>Tokuhisa</ce:surname></ce:author><ce:author><ce:given-name>K.</ce:given-name><ce:surname>Sato</ce:surname></ce:author><ce:author><ce:given-name>K.</ce:given-name><ce:surname>Satoda</ce:surname></ce:author><ce:affiliation><ce:textfn>National Institute of Animal Health, Tsukuba, Ibaraki, Japan</ce:textfn></ce:affiliation></ce:author-group><ce:date-accepted day="21" month="5" year="1980"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A coronavirus (Kakegawa isolate) isolated from a cow with epizootic diarrhea was grown in BEK-1 cells and examined for biophysical and biochemical properties. The Kakegawa isolate was able to replicate in the presence or absence of 5-iodo-2′-deoxy-uridine, indicating that its viral nucleic acid was RNA. It was highly sensitive to ether and chloroform, and moderately sensitive to trypsin and heat. It was, however, readily stabilized by treatment with cation at 50°C for 1 h. Its infectivity was slightly reduced at pH 3.0. The virus passed through a membrane filter of 200 nm pore size, but not through one of 100 nm pore size. The buoyant density of the virus was determined in a sucrose density gradient. The peak of infectivity and hemagglutinin activity was found at a density of 1.182. Neutralization and hemagglutination inhibition tests showed a close serological relationship between the Kakegawa isolate and the American strain of calf diarrhea coronavirus.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Properties of a coronavirus isolated from a cow with epizootic diarrhea</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Properties of a coronavirus isolated from a cow with epizootic diarrhea</title></titleInfo><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Akashi</namePart><affiliation>National Institute of Animal Health, Tsukuba, Ibaraki, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Inaba</namePart><affiliation>National Institute of Animal Health, Tsukuba, Ibaraki, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Miura</namePart><affiliation>National Institute of Animal Health, Tsukuba, Ibaraki, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Tokuhisa</namePart><affiliation>National Institute of Animal Health, Tsukuba, Ibaraki, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Sato</namePart><affiliation>National Institute of Animal Health, Tsukuba, Ibaraki, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Satoda</namePart><affiliation>National Institute of Animal Health, Tsukuba, Ibaraki, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1980</dateIssued><copyrightDate encoding="w3cdtf">1980</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: A coronavirus (Kakegawa isolate) isolated from a cow with epizootic diarrhea was grown in BEK-1 cells and examined for biophysical and biochemical properties. The Kakegawa isolate was able to replicate in the presence or absence of 5-iodo-2′-deoxy-uridine, indicating that its viral nucleic acid was RNA. It was highly sensitive to ether and chloroform, and moderately sensitive to trypsin and heat. It was, however, readily stabilized by treatment with cation at 50°C for 1 h. Its infectivity was slightly reduced at pH 3.0. The virus passed through a membrane filter of 200 nm pore size, but not through one of 100 nm pore size. The buoyant density of the virus was determined in a sucrose density gradient. The peak of infectivity and hemagglutinin activity was found at a density of 1.182. Neutralization and hemagglutination inhibition tests showed a close serological relationship between the Kakegawa isolate and the American strain of calf diarrhea coronavirus.</abstract><relatedItem type="host"><titleInfo><title>Veterinary Microbiology</title></titleInfo><titleInfo type="abbreviated"><title>VETMIC</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1980</dateIssued></originInfo><identifier type="ISSN">0378-1135</identifier><identifier type="PII">S0378-1135(00)X0189-7</identifier><part><date>1980</date><detail type="volume"><number>5</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="issue-pages"><start>257</start><end>355</end></extent><extent unit="pages"><start>265</start><end>276</end></extent></part></relatedItem><identifier type="istex">AB37DAE142E82FFA7C4E48D9ADFF9D5693FA9759</identifier><identifier type="ark">ark:/67375/6H6-L4KZPZXF-5</identifier><identifier type="DOI">10.1016/0378-1135(80)90025-5</identifier><identifier type="PII">0378-1135(80)90025-5</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-L4KZPZXF-5/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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