Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses.
Identifieur interne : 000622 ( Main/Exploration ); précédent : 000621; suivant : 000623Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses.
Auteurs : Ze-Gang Wu [République populaire de Chine] ; Hong-Yun Zheng [République populaire de Chine] ; Jian Gu [République populaire de Chine] ; Feng Li [République populaire de Chine] ; Rui-Long Lv [République populaire de Chine] ; Ya-Yun Deng [République populaire de Chine] ; Wan-Zhou Xu [République populaire de Chine] ; Yong-Qmg Tong [République populaire de Chine]Source :
- Current medical science [ 2523-899X ] ; 2020.
Descripteurs français
- KwdFr :
- ARN viral (analyse), ARN viral (génétique), Adulte (MeSH), Adulte d'âge moyen (MeSH), Betacoronavirus (génétique), Betacoronavirus (isolement et purification), Chine (épidémiologie), Exposition professionnelle (prévention et contrôle), Facteurs temps (MeSH), Humains (MeSH), Inactivation virale (MeSH), Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Infections à coronavirus (épidémiologie), Pandémies (MeSH), Personnel de laboratoire d'analyses médicales (MeSH), Pneumopathie virale (diagnostic), Pneumopathie virale (virologie), Pneumopathie virale (épidémiologie), Prévention des infections (méthodes), Réaction de polymérisation en chaine en temps réel (méthodes), Sujet âgé (MeSH), Techniques de diagnostic moléculaire (méthodes), Techniques de laboratoire clinique (méthodes), Température (MeSH).
- MESH :
- analyse : ARN viral.
- diagnostic : Infections à coronavirus, Pneumopathie virale.
- génétique : ARN viral, Betacoronavirus.
- isolement et purification : Betacoronavirus.
- méthodes : Prévention des infections, Réaction de polymérisation en chaine en temps réel, Techniques de diagnostic moléculaire, Techniques de laboratoire clinique.
- prévention et contrôle : Exposition professionnelle.
- virologie : Infections à coronavirus, Pneumopathie virale.
- épidémiologie : Chine, Infections à coronavirus, Pneumopathie virale.
- Adulte, Adulte d'âge moyen, Facteurs temps, Humains, Inactivation virale, Pandémies, Personnel de laboratoire d'analyses médicales, Sujet âgé, Température.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- Adult (MeSH), Aged (MeSH), Betacoronavirus (genetics), Betacoronavirus (isolation & purification), China (epidemiology), Clinical Laboratory Techniques (methods), Coronavirus Infections (diagnosis), Coronavirus Infections (epidemiology), Coronavirus Infections (virology), Humans (MeSH), Infection Control (methods), Medical Laboratory Personnel (MeSH), Middle Aged (MeSH), Molecular Diagnostic Techniques (methods), Occupational Exposure (prevention & control), Pandemics (MeSH), Pneumonia, Viral (diagnosis), Pneumonia, Viral (epidemiology), Pneumonia, Viral (virology), RNA, Viral (analysis), RNA, Viral (genetics), Real-Time Polymerase Chain Reaction (methods), Temperature (MeSH), Time Factors (MeSH), Virus Inactivation (MeSH).
- MESH :
- chemical , analysis : RNA, Viral.
- geographic , epidemiology : China.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- epidemiology : Coronavirus Infections, Pneumonia, Viral.
- genetics : Betacoronavirus, RNA, Viral.
- isolation & purification : Betacoronavirus.
- methods : Clinical Laboratory Techniques, Infection Control, Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction.
- prevention & control : Occupational Exposure.
- virology : Coronavirus Infections, Pneumonia, Viral.
- Adult, Aged, Humans, Medical Laboratory Personnel, Middle Aged, Pandemics, Temperature, Time Factors, Virus Inactivation.
Abstract
The novel Coronavirus SARS-CoV-2 caused an outbreak of pneumonia in Wuhan, Hubei province of China in January 2020. This study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for SARS-CoV-2. Twelve patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13, 2020 and throat swabs were taken. The swabs were stored at room temperature (20-25°C), then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses (56°C for 30, 45, 60 min; 65, 70, 80°C for 10, 15, 20 min). Control aliquots were stored at room temperature for 60 min. Then all aliquots were tested in a real-time fluorescence PCR using primers against SARS-CoV-2. Regardless of inactivation temperature and time, 7 of 12 cases (58.3%) tested were positive for SARS-CoV-2 by PCR, and cycle threshold values were similar. These results suggest that virus inactivation parameters exert minimal influence on PCR test results. Inactivation at 65°C for 10 min may be sufficient to ensure safe, reliable testing.
DOI: 10.1007/s11596-020-2224-y
PubMed: 32767258
PubMed Central: PMC7412769
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses.</title><author><name sortKey="Wu, Ze Gang" sort="Wu, Ze Gang" uniqKey="Wu Z" first="Ze-Gang" last="Wu">Ze-Gang Wu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Hong Yun" sort="Zheng, Hong Yun" uniqKey="Zheng H" first="Hong-Yun" last="Zheng">Hong-Yun Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Gu, Jian" sort="Gu, Jian" uniqKey="Gu J" first="Jian" last="Gu">Jian Gu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Lv, Rui Long" sort="Lv, Rui Long" uniqKey="Lv R" first="Rui-Long" last="Lv">Rui-Long Lv</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Deng, Ya Yun" sort="Deng, Ya Yun" uniqKey="Deng Y" first="Ya-Yun" last="Deng">Ya-Yun Deng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Wan Zhou" sort="Xu, Wan Zhou" uniqKey="Xu W" first="Wan-Zhou" last="Xu">Wan-Zhou Xu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Tong, Yong Qmg" sort="Tong, Yong Qmg" uniqKey="Tong Y" first="Yong-Qmg" last="Tong">Yong-Qmg Tong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China. tytsing@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32767258</idno><idno type="pmid">32767258</idno><idno type="doi">10.1007/s11596-020-2224-y</idno><idno type="pmc">PMC7412769</idno><idno type="wicri:Area/Main/Corpus">000759</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000759</idno><idno type="wicri:Area/Main/Curation">000759</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000759</idno><idno type="wicri:Area/Main/Exploration">000759</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses.</title><author><name sortKey="Wu, Ze Gang" sort="Wu, Ze Gang" uniqKey="Wu Z" first="Ze-Gang" last="Wu">Ze-Gang Wu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Hong Yun" sort="Zheng, Hong Yun" uniqKey="Zheng H" first="Hong-Yun" last="Zheng">Hong-Yun Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Gu, Jian" sort="Gu, Jian" uniqKey="Gu J" first="Jian" last="Gu">Jian Gu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Lv, Rui Long" sort="Lv, Rui Long" uniqKey="Lv R" first="Rui-Long" last="Lv">Rui-Long Lv</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Deng, Ya Yun" sort="Deng, Ya Yun" uniqKey="Deng Y" first="Ya-Yun" last="Deng">Ya-Yun Deng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Wan Zhou" sort="Xu, Wan Zhou" uniqKey="Xu W" first="Wan-Zhou" last="Xu">Wan-Zhou Xu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author><author><name sortKey="Tong, Yong Qmg" sort="Tong, Yong Qmg" uniqKey="Tong Y" first="Yong-Qmg" last="Tong">Yong-Qmg Tong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China. tytsing@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060</wicri:regionArea><wicri:noRegion>430060</wicri:noRegion></affiliation></author></analytic><series><title level="j">Current medical science</title><idno type="eISSN">2523-899X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult (MeSH)</term><term>Aged (MeSH)</term><term>Betacoronavirus (genetics)</term><term>Betacoronavirus (isolation & purification)</term><term>China (epidemiology)</term><term>Clinical Laboratory Techniques (methods)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (epidemiology)</term><term>Coronavirus Infections (virology)</term><term>Humans (MeSH)</term><term>Infection Control (methods)</term><term>Medical Laboratory Personnel (MeSH)</term><term>Middle Aged (MeSH)</term><term>Molecular Diagnostic Techniques (methods)</term><term>Occupational Exposure (prevention & control)</term><term>Pandemics (MeSH)</term><term>Pneumonia, Viral (diagnosis)</term><term>Pneumonia, Viral (epidemiology)</term><term>Pneumonia, Viral (virology)</term><term>RNA, Viral (analysis)</term><term>RNA, Viral (genetics)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Temperature (MeSH)</term><term>Time Factors (MeSH)</term><term>Virus Inactivation (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>ARN viral (génétique)</term><term>Adulte (MeSH)</term><term>Adulte d'âge moyen (MeSH)</term><term>Betacoronavirus (génétique)</term><term>Betacoronavirus (isolement et purification)</term><term>Chine (épidémiologie)</term><term>Exposition professionnelle (prévention et contrôle)</term><term>Facteurs temps (MeSH)</term><term>Humains (MeSH)</term><term>Inactivation virale (MeSH)</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>Infections à coronavirus (épidémiologie)</term><term>Pandémies (MeSH)</term><term>Personnel de laboratoire d'analyses médicales (MeSH)</term><term>Pneumopathie virale (diagnostic)</term><term>Pneumopathie virale (virologie)</term><term>Pneumopathie virale (épidémiologie)</term><term>Prévention des infections (méthodes)</term><term>Réaction de polymérisation en chaine en temps réel (méthodes)</term><term>Sujet âgé (MeSH)</term><term>Techniques de diagnostic moléculaire (méthodes)</term><term>Techniques de laboratoire clinique (méthodes)</term><term>Température (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term><term>Pneumopathie virale</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Betacoronavirus</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Betacoronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Betacoronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Betacoronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Clinical Laboratory Techniques</term><term>Infection Control</term><term>Molecular Diagnostic Techniques</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Prévention des infections</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Techniques de diagnostic moléculaire</term><term>Techniques de laboratoire clinique</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Occupational Exposure</term></keywords><keywords scheme="MESH" qualifier="prévention et contrôle" xml:lang="fr"><term>Exposition professionnelle</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term><term>Pneumopathie virale</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Chine</term><term>Infections à coronavirus</term><term>Pneumopathie virale</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Humans</term><term>Medical Laboratory Personnel</term><term>Middle Aged</term><term>Pandemics</term><term>Temperature</term><term>Time Factors</term><term>Virus Inactivation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Facteurs temps</term><term>Humains</term><term>Inactivation virale</term><term>Pandémies</term><term>Personnel de laboratoire d'analyses médicales</term><term>Sujet âgé</term><term>Température</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The novel Coronavirus SARS-CoV-2 caused an outbreak of pneumonia in Wuhan, Hubei province of China in January 2020. This study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for SARS-CoV-2. Twelve patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13, 2020 and throat swabs were taken. The swabs were stored at room temperature (20-25°C), then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses (56°C for 30, 45, 60 min; 65, 70, 80°C for 10, 15, 20 min). Control aliquots were stored at room temperature for 60 min. Then all aliquots were tested in a real-time fluorescence PCR using primers against SARS-CoV-2. Regardless of inactivation temperature and time, 7 of 12 cases (58.3%) tested were positive for SARS-CoV-2 by PCR, and cycle threshold values were similar. These results suggest that virus inactivation parameters exert minimal influence on PCR test results. Inactivation at 65°C for 10 min may be sufficient to ensure safe, reliable testing.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32767258</PMID><DateCompleted><Year>2020</Year><Month>09</Month><Day>04</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2523-899X</ISSN><JournalIssue CitedMedium="Internet"><Volume>40</Volume><Issue>4</Issue><PubDate><Year>2020</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Current medical science</Title><ISOAbbreviation>Curr Med Sci</ISOAbbreviation></Journal><ArticleTitle>Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses.</ArticleTitle><Pagination><MedlinePgn>614-617</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11596-020-2224-y</ELocationID><Abstract><AbstractText>The novel Coronavirus SARS-CoV-2 caused an outbreak of pneumonia in Wuhan, Hubei province of China in January 2020. This study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for SARS-CoV-2. Twelve patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13, 2020 and throat swabs were taken. The swabs were stored at room temperature (20-25°C), then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses (56°C for 30, 45, 60 min; 65, 70, 80°C for 10, 15, 20 min). Control aliquots were stored at room temperature for 60 min. Then all aliquots were tested in a real-time fluorescence PCR using primers against SARS-CoV-2. Regardless of inactivation temperature and time, 7 of 12 cases (58.3%) tested were positive for SARS-CoV-2 by PCR, and cycle threshold values were similar. These results suggest that virus inactivation parameters exert minimal influence on PCR test results. Inactivation at 65°C for 10 min may be sufficient to ensure safe, reliable testing.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Ze-Gang</ForeName><Initials>ZG</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Hong-Yun</ForeName><Initials>HY</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gu</LastName><ForeName>Jian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Feng</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lv</LastName><ForeName>Rui-Long</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Ya-Yun</ForeName><Initials>YY</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Wan-Zhou</ForeName><Initials>WZ</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tong</LastName><ForeName>Yong-Qmg</ForeName><Initials>YQ</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China. tytsing@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Curr Med Sci</MedlineTA><NlmUniqueID>101729993</NlmUniqueID><ISSNLinking>2523-899X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><SupplMeshList><SupplMeshName Type="Disease" UI="C000657245">COVID-19</SupplMeshName><SupplMeshName Type="Protocol" UI="C000657964">COVID-19 diagnostic testing</SupplMeshName><SupplMeshName Type="Organism" UI="C000656484">severe acute respiratory syndrome coronavirus 2</SupplMeshName></SupplMeshList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000073640" MajorTopicYN="N">Betacoronavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002681" MajorTopicYN="N" Type="Geographic">China</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019411" MajorTopicYN="N">Clinical Laboratory Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017053" MajorTopicYN="N">Infection Control</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017056" MajorTopicYN="N">Medical Laboratory Personnel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D025202" MajorTopicYN="N">Molecular Diagnostic Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016273" MajorTopicYN="N">Occupational Exposure</DescriptorName><QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058873" MajorTopicYN="N">Pandemics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011024" MajorTopicYN="N">Pneumonia, Viral</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038301" MajorTopicYN="Y">Virus Inactivation</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">COVID-19</Keyword><Keyword MajorTopicYN="N">SARS-CoV-2</Keyword><Keyword MajorTopicYN="N">real-time fluorescence PCR</Keyword><Keyword MajorTopicYN="N">throat swabs</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>04</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>05</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32767258</ArticleId><ArticleId IdType="doi">10.1007/s11596-020-2224-y</ArticleId><ArticleId IdType="pii">10.1007/s11596-020-2224-y</ArticleId><ArticleId IdType="pmc">PMC7412769</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Euro Surveill. 2020 Jan;25(3):</Citation><ArticleIdList><ArticleId IdType="pubmed">31992387</ArticleId></ArticleIdList></Reference><Reference><Citation>Epidemiol Health. 2020;42:e2020006</Citation><ArticleIdList><ArticleId IdType="pubmed">32023775</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2020 Mar 5;382(10):929-936</Citation><ArticleIdList><ArticleId IdType="pubmed">32004427</ArticleId></ArticleIdList></Reference><Reference><Citation>BMJ. 2020 Feb 18;368:m641</Citation><ArticleIdList><ArticleId IdType="pubmed">32071063</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Chem. 2020 May 1;66(5):664-666</Citation><ArticleIdList><ArticleId IdType="pubmed">32077933</ArticleId></ArticleIdList></Reference><Reference><Citation>Mil Med Res. 2020 Feb 6;7(1):4</Citation><ArticleIdList><ArticleId IdType="pubmed">32029004</ArticleId></ArticleIdList></Reference><Reference><Citation>Dermatology. 2006;212 Suppl 1:119-23</Citation><ArticleIdList><ArticleId IdType="pubmed">16490989</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Transl Med. 2020 Feb 20;9(1):19</Citation><ArticleIdList><ArticleId IdType="pubmed">32078069</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wu, Ze Gang" sort="Wu, Ze Gang" uniqKey="Wu Z" first="Ze-Gang" last="Wu">Ze-Gang Wu</name></noRegion><name sortKey="Deng, Ya Yun" sort="Deng, Ya Yun" uniqKey="Deng Y" first="Ya-Yun" last="Deng">Ya-Yun Deng</name><name sortKey="Gu, Jian" sort="Gu, Jian" uniqKey="Gu J" first="Jian" last="Gu">Jian Gu</name><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name><name sortKey="Lv, Rui Long" sort="Lv, Rui Long" uniqKey="Lv R" first="Rui-Long" last="Lv">Rui-Long Lv</name><name sortKey="Tong, Yong Qmg" sort="Tong, Yong Qmg" uniqKey="Tong Y" first="Yong-Qmg" last="Tong">Yong-Qmg Tong</name><name sortKey="Xu, Wan Zhou" sort="Xu, Wan Zhou" uniqKey="Xu W" first="Wan-Zhou" last="Xu">Wan-Zhou Xu</name><name sortKey="Zheng, Hong Yun" sort="Zheng, Hong Yun" uniqKey="Zheng H" first="Hong-Yun" last="Zheng">Hong-Yun Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/CovidSeniorV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000622 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000622 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= CovidSeniorV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32767258
|texte= Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32767258" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a CovidSeniorV1
| This area was generated with Dilib version V0.6.37. |  |