Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses.
Identifieur interne : 000759 ( Main/Corpus ); précédent : 000758; suivant : 000760Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses.
Auteurs : Ze-Gang Wu ; Hong-Yun Zheng ; Jian Gu ; Feng Li ; Rui-Long Lv ; Ya-Yun Deng ; Wan-Zhou Xu ; Yong-Qmg TongSource :
- Current medical science [ 2523-899X ] ; 2020.
English descriptors
- KwdEn :
- Adult (MeSH), Aged (MeSH), Betacoronavirus (genetics), Betacoronavirus (isolation & purification), China (epidemiology), Clinical Laboratory Techniques (methods), Coronavirus Infections (diagnosis), Coronavirus Infections (epidemiology), Coronavirus Infections (virology), Humans (MeSH), Infection Control (methods), Medical Laboratory Personnel (MeSH), Middle Aged (MeSH), Molecular Diagnostic Techniques (methods), Occupational Exposure (prevention & control), Pandemics (MeSH), Pneumonia, Viral (diagnosis), Pneumonia, Viral (epidemiology), Pneumonia, Viral (virology), RNA, Viral (analysis), RNA, Viral (genetics), Real-Time Polymerase Chain Reaction (methods), Temperature (MeSH), Time Factors (MeSH), Virus Inactivation (MeSH).
- MESH :
- chemical , analysis : RNA, Viral.
- geographic , epidemiology : China.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- epidemiology : Coronavirus Infections, Pneumonia, Viral.
- genetics : Betacoronavirus, RNA, Viral.
- isolation & purification : Betacoronavirus.
- methods : Clinical Laboratory Techniques, Infection Control, Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction.
- prevention & control : Occupational Exposure.
- virology : Coronavirus Infections, Pneumonia, Viral.
- Adult, Aged, Humans, Medical Laboratory Personnel, Middle Aged, Pandemics, Temperature, Time Factors, Virus Inactivation.
Abstract
The novel Coronavirus SARS-CoV-2 caused an outbreak of pneumonia in Wuhan, Hubei province of China in January 2020. This study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for SARS-CoV-2. Twelve patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13, 2020 and throat swabs were taken. The swabs were stored at room temperature (20-25°C), then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses (56°C for 30, 45, 60 min; 65, 70, 80°C for 10, 15, 20 min). Control aliquots were stored at room temperature for 60 min. Then all aliquots were tested in a real-time fluorescence PCR using primers against SARS-CoV-2. Regardless of inactivation temperature and time, 7 of 12 cases (58.3%) tested were positive for SARS-CoV-2 by PCR, and cycle threshold values were similar. These results suggest that virus inactivation parameters exert minimal influence on PCR test results. Inactivation at 65°C for 10 min may be sufficient to ensure safe, reliable testing.
DOI: 10.1007/s11596-020-2224-y
PubMed: 32767258
PubMed Central: PMC7412769
Links to Exploration step
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430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lv, Rui Long" sort="Lv, Rui Long" uniqKey="Lv R" first="Rui-Long" last="Lv">Rui-Long Lv</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Deng, Ya Yun" sort="Deng, Ya Yun" uniqKey="Deng Y" first="Ya-Yun" last="Deng">Ya-Yun Deng</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Wan Zhou" sort="Xu, Wan Zhou" uniqKey="Xu W" first="Wan-Zhou" last="Xu">Wan-Zhou Xu</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tong, Yong Qmg" sort="Tong, Yong Qmg" uniqKey="Tong Y" first="Yong-Qmg" last="Tong">Yong-Qmg Tong</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China. tytsing@163.com.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32767258</idno><idno type="pmid">32767258</idno><idno type="doi">10.1007/s11596-020-2224-y</idno><idno type="pmc">PMC7412769</idno><idno type="wicri:Area/Main/Corpus">000759</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000759</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses.</title><author><name sortKey="Wu, Ze Gang" sort="Wu, Ze Gang" uniqKey="Wu Z" first="Ze-Gang" last="Wu">Ze-Gang Wu</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zheng, Hong Yun" sort="Zheng, Hong Yun" uniqKey="Zheng H" first="Hong-Yun" last="Zheng">Hong-Yun Zheng</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Gu, Jian" sort="Gu, Jian" uniqKey="Gu J" first="Jian" last="Gu">Jian Gu</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lv, Rui Long" sort="Lv, Rui Long" uniqKey="Lv R" first="Rui-Long" last="Lv">Rui-Long Lv</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Deng, Ya Yun" sort="Deng, Ya Yun" uniqKey="Deng Y" first="Ya-Yun" last="Deng">Ya-Yun Deng</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Wan Zhou" sort="Xu, Wan Zhou" uniqKey="Xu W" first="Wan-Zhou" last="Xu">Wan-Zhou Xu</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tong, Yong Qmg" sort="Tong, Yong Qmg" uniqKey="Tong Y" first="Yong-Qmg" last="Tong">Yong-Qmg Tong</name><affiliation><nlm:affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China. tytsing@163.com.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Current medical science</title><idno type="eISSN">2523-899X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult (MeSH)</term><term>Aged (MeSH)</term><term>Betacoronavirus (genetics)</term><term>Betacoronavirus (isolation & purification)</term><term>China (epidemiology)</term><term>Clinical Laboratory Techniques (methods)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (epidemiology)</term><term>Coronavirus Infections (virology)</term><term>Humans (MeSH)</term><term>Infection Control (methods)</term><term>Medical Laboratory Personnel (MeSH)</term><term>Middle Aged (MeSH)</term><term>Molecular Diagnostic Techniques (methods)</term><term>Occupational Exposure (prevention & control)</term><term>Pandemics (MeSH)</term><term>Pneumonia, Viral (diagnosis)</term><term>Pneumonia, Viral (epidemiology)</term><term>Pneumonia, Viral (virology)</term><term>RNA, Viral (analysis)</term><term>RNA, Viral (genetics)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Temperature (MeSH)</term><term>Time Factors (MeSH)</term><term>Virus Inactivation (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Betacoronavirus</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Betacoronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Clinical Laboratory Techniques</term><term>Infection Control</term><term>Molecular Diagnostic Techniques</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Occupational Exposure</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Humans</term><term>Medical Laboratory Personnel</term><term>Middle Aged</term><term>Pandemics</term><term>Temperature</term><term>Time Factors</term><term>Virus Inactivation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The novel Coronavirus SARS-CoV-2 caused an outbreak of pneumonia in Wuhan, Hubei province of China in January 2020. This study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for SARS-CoV-2. Twelve patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13, 2020 and throat swabs were taken. The swabs were stored at room temperature (20-25°C), then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses (56°C for 30, 45, 60 min; 65, 70, 80°C for 10, 15, 20 min). Control aliquots were stored at room temperature for 60 min. Then all aliquots were tested in a real-time fluorescence PCR using primers against SARS-CoV-2. Regardless of inactivation temperature and time, 7 of 12 cases (58.3%) tested were positive for SARS-CoV-2 by PCR, and cycle threshold values were similar. These results suggest that virus inactivation parameters exert minimal influence on PCR test results. Inactivation at 65°C for 10 min may be sufficient to ensure safe, reliable testing.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32767258</PMID><DateCompleted><Year>2020</Year><Month>09</Month><Day>04</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2523-899X</ISSN><JournalIssue CitedMedium="Internet"><Volume>40</Volume><Issue>4</Issue><PubDate><Year>2020</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Current medical science</Title><ISOAbbreviation>Curr Med Sci</ISOAbbreviation></Journal><ArticleTitle>Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses.</ArticleTitle><Pagination><MedlinePgn>614-617</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11596-020-2224-y</ELocationID><Abstract><AbstractText>The novel Coronavirus SARS-CoV-2 caused an outbreak of pneumonia in Wuhan, Hubei province of China in January 2020. This study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for SARS-CoV-2. Twelve patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13, 2020 and throat swabs were taken. The swabs were stored at room temperature (20-25°C), then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses (56°C for 30, 45, 60 min; 65, 70, 80°C for 10, 15, 20 min). Control aliquots were stored at room temperature for 60 min. Then all aliquots were tested in a real-time fluorescence PCR using primers against SARS-CoV-2. Regardless of inactivation temperature and time, 7 of 12 cases (58.3%) tested were positive for SARS-CoV-2 by PCR, and cycle threshold values were similar. These results suggest that virus inactivation parameters exert minimal influence on PCR test results. Inactivation at 65°C for 10 min may be sufficient to ensure safe, reliable testing.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Ze-Gang</ForeName><Initials>ZG</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Hong-Yun</ForeName><Initials>HY</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gu</LastName><ForeName>Jian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Feng</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lv</LastName><ForeName>Rui-Long</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Ya-Yun</ForeName><Initials>YY</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Wan-Zhou</ForeName><Initials>WZ</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tong</LastName><ForeName>Yong-Qmg</ForeName><Initials>YQ</Initials><AffiliationInfo><Affiliation>Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China. tytsing@163.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Curr Med Sci</MedlineTA><NlmUniqueID>101729993</NlmUniqueID><ISSNLinking>2523-899X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><SupplMeshList><SupplMeshName Type="Disease" UI="C000657245">COVID-19</SupplMeshName><SupplMeshName 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Diagnostic Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016273" MajorTopicYN="N">Occupational Exposure</DescriptorName><QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058873" MajorTopicYN="N">Pandemics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011024" MajorTopicYN="N">Pneumonia, Viral</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName 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